Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1269 |
| PDBID: | 5DO2 |
| Chains: | CD_B |
| Organism: | Middle East respiratory syndrome-related coronavirus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.41 |
| Reference: | 10.1038/cr.2015.113 |
| Antibody | |
| Antibody: | 4C2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | MERS Co-V RBD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >5DO2_C|Chain B[auth C], E[auth H]|4C2 heavy chain|Mus musculus (10090) DVKLVESGGGLVKPGGSLKLSCAASGFTFSSYTMSWVRQTPEKRLEWVATISSGGSYTYYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYYCTRDGNDYDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCG |
Light Chain: D
Mutation: NULL
| >5DO2_D|Chain C[auth D], F[auth L]|4C2 light chain|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTISCRASQDISNYLNWYQQKPDGTVKLLIYYTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >5DO2_B|Chain A, D[auth B]|S protein|Middle East respiratory syndrome coronavirus (1335626) ADGIQEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: SER31 TYR32 THR33 THR50 SER52 SER53 GLY54 SER56 TYR59 ARG98 ASP99 GLY100 ASN101 ASP102 ASP104 D: GLN27 ASP28 SER30 TYR32 TYR49 TYR50 ARG53 LEU54 HIS55 SER56 GLY91 ASN92 THR93 LEU94 ARG96 B: GLY391 THR392 PRO394 TYR397 ASN398 LYS400 LEU495 LYS496 TYR523 PRO525 VAL527 SER528 ILE529 VAL530 PRO531 SER532 THR533 TRP535 GLU536 ASP539 TYR540 TYR541 ARG542 LYS543 GLN544 LEU545 SER546 GLU549 TRP553 LEU554 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
