Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1255 |
| PDBID: | 5DA0 |
| Chains: | B_A |
| Organism: | Deinococcus geothermalis DSM 11300, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1038/nsmb.3091 |
| Antibody | |
| Antibody: | Nb5776 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Sulphate transporter SLC26Dg |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >5DA0_B|Chain B|Nanobody|Lama glama (9844) GPSQVQLQESGGGLVQAGGSLRLSCAASGRTFSSDVMGWFRQAPGKEREFVAAVTRSGGKSYNADSVKGRFTISRDNAKNTVSLQMNSLKPEDTAVYYCAAGDTAITSWYGYDYWGQGTQVTVS |
Antigen
Chain: A
Mutation: NULL
| >5DA0_A|Chain A|Sulphate transporter|Deinococcus geothermalis (strain DSM 11300) (319795) MTVHSPRFDLRQYQREWFANPRKDVLAGIVVALALIPEAIAFSIIAGVDPQVGLYASFIIALITAFLGGRPGMISAATGAMALLMTGLVKDHGIQYLFAATVLTGVLQVVFGWAKLARYLKFVPRSVMVGFVNALAILIFMAQLPQFVGANWQMYAMVAAGLAIIYLLPLVFKAMPSALVAIVVLTVVAVVTGADVKTVGDMGTLPTALPHFQFPQVPLTFETLAIIFPVALTLSLVGLLESLLTAQLIDERTDTTSDKNVESRGQGVANIVTGFFGGMAGCAMIGQSMINVTSGGRGRLSTFVAGAFLMVLILALQPLLVQIPMAALVAVMMVVAISTFDWGSLRTLTVFPKGETVVMLATVAVTVFTHDLSLGVLIGVVLSALFFARKVSQLSQVTPVDEVDGTRTYRVRGQLFFVSTHDFLHQFDFTHPARRVVIDLSDAHFWDGSAVGALDKVMLKFMRQGTSVELRGLNAASATLVERLAVHDKPDALDRMGGHSLGVLP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ASP35 VAL36 LYS46 GLU47 ARG48 GLU49 PHE50 ALA53 THR55 ARG56 SER57 GLY58 GLY59 LYS60 SER61 TYR62 ASN63 ALA64 ASP65 VAL67 GLY69 THR104 ALA105 ILE106 THR107 SER108 TRP109 TYR110 A: ARG435 PHE445 GLY448 VAL451 LEU454 ASP455 MET458 LEU459 MET462 ARG463 GLY465 SER467 VAL468 GLU469 LEU470 ARG471 GLY472 LEU473 ASN474 SER477 LEU480 GLU482 ARG483 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
