Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1247 |
| PDBID: | 5D96 |
| Chains: | JI_D |
| Organism: | Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1093/protein/gzv067 |
| Antibody | |
| Antibody: | Fab492gen Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mus musculus Quiescin sulfhydryl oxidase-1(Trx) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >5D96_J|Chain C, F[auth J]|Heavy chain of Fab fragment from an antibody targeting mouse and human QSOX1|Mus musculus (10090) QVQLKQSGPGLVAPSQSLSITCTVSGFSLTGYSVIWVRQSPGKGLEWLGMIWGDGRTEYKSALKSRLSITKDNSKSQVFLKMNSLQTDDTARYFCASDSMDPGSFAYWGQGTLVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: I
Mutation: NULL
| >5D96_I|Chain B, E[auth I]|Light chain of Fab fragment from an antibody targeting mouse and human QSOX1|Mus musculus (10090) DVVMTQTHKFMSTSVGDRVSITCKASQDVSGAVAWYQQKSGQSPKLLISMASQRYTGVPDRFTGSGSGTDFTFTISSVQAEDLAVYYCQQHYAIPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: D
Mutation: NULL
| >5D96_D|Chain A, D|Sulfhydryl oxidase 1|Mus musculus (10090) GSHMSVLYSSSDPLTLLDADSVRPTVLGSSSAWAVEFFASWCGHCIAFAPTWKELANDVKDWRPALNLAVLDCAEETNSAVCREFNIAGFPTVRFFQAFTKNGSGATLPGAGANVQTLRMRLIDALESHRDTWPPACPPLEPAKLNDIDGFFTRNKADYLALVFEREDSYLGREVTLDLSQYHAVAVRRVLNTESDLVNKFGVTDFPSCYLLLRNGSVSRVPVLVESRSFYTSYLRGLPGLTRD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: SER30 GLY31 ALA32 MET50 GLN53 HIS91 TYR92 ALA93 ILE94 LEU96 J: THR30 GLY31 TYR32 SER33 MET50 TRP52 GLY53 ASP54 ARG56 GLU58 ASP98 SER99 MET100 ASP101 PRO102 GLY103 D: TRP72 CYS73 GLY74 HIS75 ALA78 ALA105 SER110 ARG114 PHE116 ASN117 ILE118 ALA119 GLY120 PHE121 PRO122 THR123 ARG125 SER135 GLY136 ALA137 THR138 LEU139 PRO140 GLY141 ALA142 GLY143 THR148 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
