Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1244 |
| PDBID: | 5D8J |
| Chains: | HL_A |
| Organism: | Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1126/scitranslmed.aac6336 |
| Antibody | |
| Antibody: | HA3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Fatty acid-binding protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5D8J_H|Chain B[auth H]|HA3 Fab Heavy Chain|Mus musculus (10090) QVQLQQPGAELVKPGASVKLSCKASGYTFTSNWITWVKQRPGQGLEWIGDIYPGSGSTTNNEKFKSKATLTVDTSSSTAYMQLSSLTSEDSAVYYCARLRGYYDYFDFWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >5D8J_L|Chain C[auth L]|HA3 Fab Light Chain|Mus musculus (10090) EVVLTQSPALMAASPGEKVTITCSVSSSISSSNLHWYQQKSETSPKPWIYGTSNLASGVPVRFSGSGSGTSYSLTISSMEAEDAATYYCQQWSHYPLTFGAGTKLELKRTDAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >5D8J_A|Chain A|Fatty acid-binding protein, adipocyte|Mus musculus (10090) GSMCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDLVTIRSESTFKNTEISFKLGVEFDEITADDRKVKSIITLDGGALVQVQKWDGKSTTIKRKRDGDKLVVECVMKGVTSTRVYERA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER31 TRP33 ASP50 TYR52 GLY54 SER55 SER57 THR58 THR59 LEU99 ARG100 GLY101 TYR102 TYR103 ASP104 L: SER30 SER32 ASN33 ASN54 TRP92 SER93 HIS94 TYR95 A: LYS22 VAL24 GLY25 VAL26 GLY27 PHE28 ALA29 THR30 ARG31 LYS32 VAL33 MET36 PHE58 LYS59 ASP77 ASP78 ARG79 LYS80 TRP98 ASP99 LYS101 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
