Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1241 |
| PDBID: | 5D71 |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.25 |
| Reference: | 10.1080/19420862.2015.1099774 |
| Antibody | |
| Antibody: | MOR04302 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Granulocyte-macrophage colony-stimulating factor |
| Antigen mutation: | No |
| Durg Target: | P04141 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5D71_H|Chain B[auth H]|Immunglobulin G1 Fab fragment, heavy chain|Homo sapiens (9606) QVQLVESGGGLVQPGGSLRLSCAASGFTFSSYWMNWVRQAPGKGLEWVSGISYSGSETYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARGFGTDFWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSEFDYKDDDDKGAPHHHHHH |
Light Chain: L
Mutation: NULL
| >5D71_L|Chain C[auth L]|Immunglobulin G1 Fab fragment, light chain|Homo sapiens (9606) DIELTQPPSVSVAPGQTARISCSGDSIGKKYAYWYQQKPGQAPVLVIYKKRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCSAWGDKGMVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEA |
Antigen
Chain: A
Mutation: NULL
| >5D71_A|Chain A|Granulocyte-macrophage colony-stimulating factor|Homo sapiens (9606) MWLQSLLLLGTVACSISAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER31 TYR32 TRP33 TRP47 TYR52 GLU56 TYR58 GLY95 PHE96 GLY97 ASP101 L: GLY29 LYS30 LYS31 TYR32 TYR34 TRP91 GLY92 ASP93 GLY95 A: THR10 GLN11 PRO12 TRP13 HIS15 VAL16 ASN17 GLN20 GLU21 ARG23 ARG24 HIS83 GLN86 HIS87 PRO89 PHE119 ASP120 CYS121 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
