Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1235 |
| PDBID: | 5CUS |
| Chains: | KO_D |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1073/pnas.1518361112 |
| Antibody | |
| Antibody: | KTN3379 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Receptor tyrosine-protein kinase erbB-3 |
| Antigen mutation: | No |
| Durg Target: | P21860 |
Sequence information
Antibody
Heavy Chain: K
Mutation: NULL
| >5CUS_K|Chain E[auth H], G[auth I], H[auth J], I[auth K]|IgG H chain|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFTFSYYYMQWVRQAPGKGLEWVSYIGSSGGVTNYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARVGLGDAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: O
Mutation: NULL
| >5CUS_O|Chain F[auth L], J[auth M], K[auth N], L[auth O]|Fab LC region of KTN3379|Homo sapiens (9606) QSVLTQPPSASGTPGQRVTISCSGSLSNIGLNYVSWYQQLPGTAPKLLISRNNQRPSGVPDRFSGSKSGTSASLAISGLRSEDEADYYCAAWDDSPPGEAFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: D
Mutation: NULL
| >5CUS_D|Chain A, B, C, D|Receptor tyrosine-protein kinase erbB-3|Homo sapiens (9606) SEVGNSQAVCPGTLNGLSVTGDAENQYQTLYKLYERCEVVMGNLEIVLTGHNADLSFLQWIREVTGYVLVAMNEFSTLPLPNLRVVRGTQVYDGKFAIFVMLNYNTNSSHALRQLRLTQLTEILSGGVYIEKNDKLCHMDTIDWRDIVRDRDAEIVVKDNGRSCPPCHEVCKGRCWGPGSEDCQTLTKTICAPQCNGHCFGPNPNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPRCPQPLVYNKLTFQLEPNPHTKYQYGGVCVASCPHNFVVDQTSCVRACPPDKMEVDKNGLKMCEPCGGLCPKACEGTGSGSRFQTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRIYISANRQLCYHHSLNWTKVLRGPTEERLDIKHNRPRRDCVAEGKVCDPLCSSGGCWGPGPGQCLSCRNYSRGGVCVTHCNFLNGEPREFAHEAECFSCHPECQPMEGTATCNGSGSDTCAQCAHFRDGPHCVSSCPHGVLGAKGPIYKYPDVQNECRPCHENCTQGCKGPELQDCLGQTLVLIGKTHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| K: SER30 TYR31 TYR32 TYR33 GLY52 SER53 SER54 GLY55 GLY56 VAL57 THR58 VAL99 GLY100 LEU101 GLY102 ASP103 O: SER25 LEU26 LEU31 ASN32 TYR33 ARG51 LYS67 THR70 TRP92 ASP94 D: MET291 GLU292 ASP294 PRO309 LYS310 ALA311 GLU313 SER317 GLY318 SER319 LYS337 LEU339 GLU373 THR375 GLY400 GLY401 ARG402 SER403 LEU404 ASN406 ARG407 GLY408 GLU429 SER431 ALA432 GLY433 ARG456 GLY457 PRO458 ARG462 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
