Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1224 |
| PDBID: | 5CBE |
| Chains: | AB_E |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.40 |
| Reference: | 10.1074/jbc.M115.688010 |
| Antibody | |
| Antibody: | E10 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | C-X-C motif chemokine 13 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >5CBE_A|Chain A, C|E10 heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAREPDYYDSSGYYPIDAFDIWGQGTTVTVSSGGGGSGGGGSGGGGS |
Light Chain: B
Mutation: NULL
| >5CBE_B|Chain B, D|E10 light chain|Homo sapiens (9606) QSALTQPASVSASPGQSITISCTGTSSDVGAYDWVSWYQQHPGKAPKLLIFDVNNRPSGVSHRFSGSKSGNTASLTISGLQAEDEADYYCASATLLDTYVFGTGTKVTVLGDQEPKSSDKTH |
Antigen
Chain: E
Mutation: NULL
| >5CBE_E|Chain E, F|C-X-C motif chemokine 13|Homo sapiens (9606) MVLEVYYTSLRCRCVQESSVFIPRRFIDRIQILPRGNGCPRKEIIVWKKNKSIVCVDPQAEWIQRMMEVLRKRSSSTLPVPVFKRKIP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER30 TYR32 ALA33 TRP47 GLY50 PRO52 ILE53 PHE54 THR56 ASN58 GLU73 GLU95 ASP97 TYR99 B: TYR30 ASP31 TRP32 ALA91 THR92 LEU93 THR95 TYR96 E: PHE18 ILE19 PRO20 ARG21 ARG22 PHE23 ILE24 ASP25 ARG26 ILE27 GLN28 ILE29 LYS46 ASN47 TRP59 ARG62 MET63 GLU65 VAL66 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
