Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1219 |
| PDBID: | 5C2U |
| Chains: | B_A |
| Organism: | Camelus dromedarius, Xenopus laevis |
| Method: | XRD |
| Resolution (Å): | 1.55 |
| Reference: | 10.1126/science.aac7420 |
| Antibody | |
| Antibody: | Nb10 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Nup54 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >5C2U_B|Chain A[auth B]|Nanobody|Camelus dromedarius (9838) AGTGSGQVQLQESGGGLVQPGGSLRLSCVVSGDYYAIGWFRQAPGKEREGVAAISSRDGSTYYPDAVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAADRRQRWGPYYYLSALEYVYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: NULL
| >5C2U_A|Chain B[auth A]|Nup54|Xenopus laevis (8355) GSMGTNKDEDGLISLIFNKKESDIRGQQQQLVESLHKVLGGHQTLTVNVEGVKTKADNQTEVIIYVVERSPNGTSRRVGASALFSYFEQAHIKANMQSLGVTGAMAQTELSPVQIKQLIQNPLSGVDPIIWEQAKVDNPDPER |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ASP26 TYR27 TYR28 SER49 ARG50 ASP51 ASP95 ARG96 ARG97 GLN98 ARG99 TRP100 GLY101 PRO102 TYR103 TYR104 LEU109 GLU110 VAL112 TYR113 A: SER220 ILE222 LYS261 GLN265 GLU267 SER287 PHE290 SER291 PHE293 GLU294 GLN295 LYS299 MET302 GLN303 VAL307 THR308 GLY309 ALA310 MET311 ALA312 GLN313 THR314 GLU315 LEU316 PRO318 ILE321 LYS322 LEU324 ILE325 PRO328 LEU329 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
