Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1213 |
| PDBID: | 5BV7 |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.45 |
| Reference: | 10.1074/jbc.M115.672790 |
| Antibody | |
| Antibody: | 27C3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Phosphatidylcholine-sterol acyltransferase |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5BV7_H|Chain C[auth H]|27C3 heavy chain|Homo sapiens (9606) QVQLQESGPGLVKPSQTLSLTCTVSGASISSGGYNWSWIRQHPGKGLEWIGYIYYSGSTYYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARERGYCSSTSCSRVMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDEVD |
Light Chain: L
Mutation: NULL
| >5BV7_L|Chain B[auth L]|27C3 light chain|Homo sapiens (9606) SSELTQDPAVSVALGQTVRITCQGDSLRSYYASWYQQKPGQAPVLVIYGKNNRPSGIPDRFSGSSSGNTASLTITGAQAEDEADYYCNSRDNIGNHQVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >5BV7_A|Chain A|Phosphatidylcholine-sterol acyltransferase|Homo sapiens (9606) FWLFDVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPEENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: SER29 TYR30 ARG90 ASP91 ASN92 ILE93 GLY94 ASN95 H: SER30 SER31 GLY32 GLY33 TYR52 TYR54 TYR55 SER56 GLY57 SER58 THR59 TYR60 TYR61 LYS66 ILE71 SER72 GLY102 CYS104 SER105 SER106 THR107 SER108 CYS109 SER110 ARG111 A: ARG256 MET257 ALA258 PRO260 TYR315 VAL317 GLY318 LEU319 PRO320 TYR341 GLU342 ASP343 THR350 ARG351 GLU354 LEU355 GLY357 LEU358 GLN360 ARG362 VAL367 HIS368 LEU369 PRO371 HIS373 HIS389 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
