Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1205 |
| PDBID: | 5BO1 |
| Chains: | HL_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.56 |
| Reference: | 10.1038/nature15715 |
| Antibody | |
| Antibody: | anti-Jag1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Protein jagged-1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5BO1_H|Chain C[auth H], D[auth I]|Fab heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTFSNYGIHWVRQAPGKGLEWVGWITPDGGYTDYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARAGTLFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >5BO1_L|Chain E[auth L], F[auth M]|Fab light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYTTATTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >5BO1_B|Chain A, B|Protein jagged-1|Homo sapiens (9606) TCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGICNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 ASN31 TYR32 HIS35 TRP50 THR52 ASP54 GLY55 TYR57 THR58 ASP59 ALA99 GLY100 THR101 LEU102 PHE103 L: SER30 THR31 ALA32 LEU46 TYR49 SER50 SER52 PHE53 LEU54 TYR55 SER56 TYR91 TYR92 THR93 THR94 THR96 B: TYR190 ARG201 PRO202 ARG203 ASP204 ASP205 PHE206 PHE207 HIS209 ALA211 CYS212 THR219 MET221 GLU222 TRP224 SER244 CYS245 LYS246 LEU247 PRO248 ASP250 ARG252 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
