Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1204 |
| PDBID: | 5BK2 |
| Chains: | CD_B |
| Organism: | Escherichia coli K-12, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1074/jbc.RA117.000656 |
| Antibody | |
| Antibody: | sAB Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Maltose binding periplasmic protein |
| Antigen mutation: | No |
| Durg Target: | P0AEX9 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >5BK2_C|Chain C, D[auth H]|sAB Heavy Chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNIYSSSIHWVRQAPGKGLEWVASISSYSGYTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARYKYPYWSWYYYWGGMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: D
Mutation: NULL
| >5BK2_D|Chain E[auth D], F[auth L]|sAB Light Chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQYYYGYPITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >5BK2_B|Chain A, B|Maltose-binding periplasmic protein|Escherichia coli (83333) MKHHHHHHHHHHSSDYKDDDDKGENLYFQGSKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: TYR33 SER34 TYR57 TYR104 PRO105 TRP107 SER108 TRP109 TYR110 TYR111 TYR112 TRP113 D: SER29 VAL30 SER31 SER32 ALA33 SER51 SER66 ARG67 SER68 GLY69 THR70 TYR93 TYR94 TYR96 B: GLU45 PRO48 GLN49 ALA51 ALA52 THR53 ARG66 GLY69 TYR70 GLN72 SER73 GLY74 LEU75 ILE178 PRO334 GLN335 SER337 ALA338 TYR341 ALA342 THR345 ASN349 ARG354 GLN355 GLU359 LYS362 ASP363 ALA364 GLN365 THR366 ASN367 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
