Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1201 |
| PDBID: | 5BK1 |
| Chains: | HL_A |
| Organism: | Homo sapiens, Escherichia coli |
| Method: | XRD |
| Resolution (Å): | 2.15 |
| Reference: | 10.1074/jbc.RA117.000656 |
| Antibody | |
| Antibody: | 5BK1 synthetic-Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Maltose binding periplasmic protein |
| Antigen mutation: | No |
| Durg Target: | P0AEX9 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5BK1_H|Chain A[auth C], C[auth H]|Synthetic antibody, Fab fragment, Heavy Chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNVYYSSIHWVRQAPGKGLEWVASIYSYYGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCAREYHSYVYEPPLYGMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >5BK1_L|Chain B[auth D], D[auth L]|Synthetic antibody, Fab fragment, Light Chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGSAPSLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSPQGYLVTFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWSVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSSADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >5BK1_A|Chain E[auth A], F[auth B]|Maltose binding protein|Escherichia coli (562) MKHHHHHHHHHHSSDYKDDDDKGENLYFQGSKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLY29 PHE30 ASN31 TYR33 TYR34 SER35 SER56 TYR57 TYR58 GLY59 SER60 ARG101 TYR103 HIS104 SER105 TYR106 VAL107 TYR108 GLU109 PRO110 TYR113 ASP116 TYR117 L: VAL30 SER31 SER32 TYR56 SER57 ARG67 PRO93 GLN94 GLY95 TYR96 A: ASN12 ASP14 LYS15 ASP41 LYS42 GLU44 GLU45 GLN49 TRP62 ALA63 ASP65 ARG66 GLY69 GLU111 GLN152 GLU153 PRO154 TYR155 PHE156 ASP207 ASP209 TYR210 SER211 ILE212 ALA215 TRP230 MET330 PRO334 MET336 SER337 TRP340 TYR341 ARG344 ILE348 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
