Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 119 |
| PDBID: | 1NSN |
| Chains: | HL_S |
| Organism: | Mus musculus, Staphylococcus aureus |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1006/jmbi.1995.0573 |
| Antibody | |
| Antibody: | N10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | STAPHYLOCOCCAL NUCLEASE |
| Antigen mutation: | No |
| Durg Target: | P00644 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >1NSN_H|Chain B[auth H]|IGG FAB (IGG1, KAPPA)|Mus musculus (10090) DVQLQESGPGLVKPSQSLSLTCTVTGYSITSDYAWNWIRQFPGNKLEWMGYITYSGTTSYNPSLKSRISISRDTSKNQFFMQLNSVTTEDTGTFYCTRGNGDWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKI |
Light Chain: L
Mutation: NULL
| >1NSN_L|Chain A[auth L]|IGG FAB (IGG1, KAPPA)|Mus musculus (10090) DIVLTQSPSSLAVSLGQRATISCRASQSVSTSSFRYMHWYQQKPGQPPRLLIKYASNLESGVPARFSGSGSGTDFTLNIHPVEEEDTATYYCQHSWEIPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: S
Mutation: NULL
| >1NSN_S|Chain C[auth S]|STAPHYLOCOCCAL NUCLEASE|Staphylococcus aureus (1280) ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSENDADSGQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: THR27 SER28 SER29 PHE30 TYR32 LYS49 TYR50 ASN53 TRP92 GLU93 ILE94 TYR96 H: ASP1 TYR27 THR30 SER31 ALA34 ASN35 TYR50 THR52 TYR53 SER54 THR56 THR57 SER58 GLY95 ASN96 S: LYS9 PRO56 GLU57 ALA60 PHE61 LYS64 MET65 ASN68 LYS70 GLU73 TYR93 ASP95 GLY96 LYS97 MET98 ARG105 GLN106 LEU108 THR120 HIS121 GLN123 HIS124 LYS127 GLN131 LYS134 GLU135 LEU137 ASN138 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
