Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1187 |
| PDBID: | 5ANM |
| Chains: | DC_F |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.85 |
| Reference: | 10.4161/mabs.28394 |
| Antibody | |
| Antibody: | MEDI4212 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | IG EPSILON CHAIN C REGION |
| Antigen mutation: | No |
| Durg Target: | P01854 |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >5ANM_D|Chain B, D, H|IMMUNOGLOBULIN G|HOMO SAPIENS (9606) EVQLVQSGAEVKKPGATVKISCKVYGYIFTDYNIYWVRQAPGKGLEWMGLIDPDNGETFYAEKFQGRATMTADTSSDRAYMELSSLRFEDTAVYYCATVMGKWIKGGYDYWGRGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: C
Mutation: NULL
| >5ANM_C|Chain A, C, I[auth L]|IMMUNOGLOBULIN G|HOMO SAPIENS (9606) QSVLTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKLLIYDNFNRPSGVPDRFSGSKSGTSASLAITGLQAEDEADYYCQSYDSPTLTSPFGTGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: F
Mutation: NULL
| >5ANM_F|Chain E, F, G|IG EPSILON CHAIN C REGION|HOMO SAPIENS (9606) ADPCDSNPRGVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGKDYKDDDDKAAHHHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: VAL5 SER7 GLU10 LYS19 SER21 LYS23 TYR25 THR30 ASP52 PRO53 ASP54 ASN55 GLY56 GLU57 ALA72 ASP73 THR74 SER75 SER76 ARG78 TYR80 TRP103 F: LEU340 ARG342 ARG419 ARG427 ALA428 LEU429 MET430 ARG431 SER432 THR433 THR434 LYS435 THR436 SER437 GLY438 GLU472 LYS497 PHE503 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
