Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1182 |
| PDBID: | 5A8H |
| Chains: | LK_G |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 23.00 |
| Reference: | 10.1016/j.cell.2015.08.035 |
| Antibody | |
| Antibody: | 8ANC195 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: L
Mutation: NULL
| >5A8H_L|Chain F, L, R|FAB OF BROADLY NEUTRALIZING ANTIBODY 8ANC195 VARIANT G52K5|HOMO SAPIENS (9606) QIHLVQSGTEVKKPGSSVTVSCKAYGVNTFGLYAVNWVRQAPGQSLEYIGQIWRWKSSASHHFRGRVLISAVDLTGSSPPISSLEIKNLTSDDTAVYFCTTTSTYDKWSGLHHDGVMAFSSWGQGTLISVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHHHHHH |
Light Chain: K
Mutation: NULL
| >5A8H_K|Chain E, K, Q|FAB OF BROADLY NEUTRALIZING ANTIBODY 8ANC195 VARIANT G52K5|HOMO SAPIENS (9606) DIQMTQSPSTLSASIGDTVRISCRASQSITGNWVAWYQQRPGKAPRLLIYRGAALLGGVPSRFSGSAAGTDFTLTIGNLQAEDFGTFYCQQYDTYPGTFGQGTKVEVKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: G
Mutation: NULL
| >5A8H_G|Chain A, G, M|HIV-1 GP120|HUMAN IMMUNODEFICIENCY VIRUS 1 (11676) GARSEVKLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVGAGSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFTNNAKTIIVQLNESVVINCTGAGHCNLSKTQWENTLEQIAIKLKEQFGNNKTIIFNPSSGGDPEIVTHSFNCGGEFFYCNSTQLFTWNDTRKLNNTGRNITLPCRIKQIINMWQEVGKAMYAPPIRGQIRCSSNITGLLLTRDGGKDTNGTEIFRPGGGDMRDNWRSELYKYKVVKIE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| K: ARG50 L: GLN1 ILE2 TYR25 GLY26 VAL27 ASN28 THR29 PHE30 GLY31 LEU32 TYR33 ILE51 TRP52 ARG54 TRP55 VAL72 ASP73 LEU74 THR75 GLY76 SER96 THR97 TYR98 ASP99 SER101 G: GLU87 ASN88 VAL89 THR90 GLU91 ASN92 PHE93 ASN94 TRP96 LYS97 ALA224 LEU226 ASN229 ASP230 LYS232 PHE233 ASN234 THR236 GLY237 PRO238 CYS239 THR240 ASN241 THR244 VAL271 ILE272 ARG273 SER274 GLU275 ASN276 PHE277 THR278 GLU351 GLN352 PHE353 LYS487 VAL489 LYS490 ILE491 GLU492 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
