Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1181 |
| PDBID: | 5A8H |
| Chains: | RQ_M |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 23.00 |
| Reference: | 10.1016/j.cell.2015.08.035 |
| Antibody | |
| Antibody: | 8ANC195 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: R
Mutation: NULL
| >5A8H_R|Chain F, L, R|FAB OF BROADLY NEUTRALIZING ANTIBODY 8ANC195 VARIANT G52K5|HOMO SAPIENS (9606) QIHLVQSGTEVKKPGSSVTVSCKAYGVNTFGLYAVNWVRQAPGQSLEYIGQIWRWKSSASHHFRGRVLISAVDLTGSSPPISSLEIKNLTSDDTAVYFCTTTSTYDKWSGLHHDGVMAFSSWGQGTLISVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHHHHHH |
Light Chain: Q
Mutation: NULL
| >5A8H_Q|Chain E, K, Q|FAB OF BROADLY NEUTRALIZING ANTIBODY 8ANC195 VARIANT G52K5|HOMO SAPIENS (9606) DIQMTQSPSTLSASIGDTVRISCRASQSITGNWVAWYQQRPGKAPRLLIYRGAALLGGVPSRFSGSAAGTDFTLTIGNLQAEDFGTFYCQQYDTYPGTFGQGTKVEVKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: M
Mutation: NULL
| >5A8H_M|Chain A, G, M|HIV-1 GP120|HUMAN IMMUNODEFICIENCY VIRUS 1 (11676) GARSEVKLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVGAGSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFTNNAKTIIVQLNESVVINCTGAGHCNLSKTQWENTLEQIAIKLKEQFGNNKTIIFNPSSGGDPEIVTHSFNCGGEFFYCNSTQLFTWNDTRKLNNTGRNITLPCRIKQIINMWQEVGKAMYAPPIRGQIRCSSNITGLLLTRDGGKDTNGTEIFRPGGGDMRDNWRSELYKYKVVKIE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Q: TYR49 ARG50 R: GLN1 ILE2 CYS22 LYS23 ALA24 TYR25 GLY26 VAL27 ASN28 THR29 PHE30 GLY31 LEU32 TYR33 GLN50 ILE51 TRP52 ARG54 TRP55 LYS56 SER70 ALA71 VAL72 ASP73 LEU74 THR75 GLY76 ILE77 THR94 SER96 THR97 TYR98 ASP99 M: LEU86 GLU87 ASN88 VAL89 THR90 GLU91 ASN92 PHE93 ASN94 MET95 TRP96 LYS97 ASN98 ALA219 GLY222 PHE223 ALA224 ILE225 LEU226 CYS228 ASN229 ASP230 LYS232 PHE233 ASN234 THR236 GLY237 PRO238 CYS239 THR240 ASN241 THR244 VAL245 GLN246 VAL271 ILE272 ARG273 SER274 GLU275 ASN276 PHE277 LYS282 THR283 ILE284 GLN352 LYS485 LYS487 VAL488 VAL489 LYS490 ILE491 GLU492 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
