Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1180 |
| PDBID: | 5A8H |
| Chains: | FE_A |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 23.00 |
| Reference: | 10.1016/j.cell.2015.08.035 |
| Antibody | |
| Antibody: | 8ANC195 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >5A8H_F|Chain F, L, R|FAB OF BROADLY NEUTRALIZING ANTIBODY 8ANC195 VARIANT G52K5|HOMO SAPIENS (9606) QIHLVQSGTEVKKPGSSVTVSCKAYGVNTFGLYAVNWVRQAPGQSLEYIGQIWRWKSSASHHFRGRVLISAVDLTGSSPPISSLEIKNLTSDDTAVYFCTTTSTYDKWSGLHHDGVMAFSSWGQGTLISVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHHHHHH |
Light Chain: E
Mutation: NULL
| >5A8H_E|Chain E, K, Q|FAB OF BROADLY NEUTRALIZING ANTIBODY 8ANC195 VARIANT G52K5|HOMO SAPIENS (9606) DIQMTQSPSTLSASIGDTVRISCRASQSITGNWVAWYQQRPGKAPRLLIYRGAALLGGVPSRFSGSAAGTDFTLTIGNLQAEDFGTFYCQQYDTYPGTFGQGTKVEVKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >5A8H_A|Chain A, G, M|HIV-1 GP120|HUMAN IMMUNODEFICIENCY VIRUS 1 (11676) GARSEVKLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVGAGSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFTNNAKTIIVQLNESVVINCTGAGHCNLSKTQWENTLEQIAIKLKEQFGNNKTIIFNPSSGGDPEIVTHSFNCGGEFFYCNSTQLFTWNDTRKLNNTGRNITLPCRIKQIINMWQEVGKAMYAPPIRGQIRCSSNITGLLLTRDGGKDTNGTEIFRPGGGDMRDNWRSELYKYKVVKIE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: TYR25 GLY26 VAL27 ASN28 THR29 GLY31 LEU32 TYR33 TRP52 ARG54 TRP55 LYS56 ASP73 LEU74 THR75 GLY76 SER96 THR97 TYR98 ASP99 A: VAL89 THR90 GLU91 ASN92 PHE93 ASN94 TRP96 LYS97 ASN98 ASN99 ASP230 LYS232 PHE233 ASN234 THR236 GLY237 PRO238 THR240 SER274 GLU275 ASN276 PHE277 THR278 GLU351 GLN352 PHE353 LYS357 ARG456 ASP461 GLU466 LYS487 VAL489 LYS490 GLU492 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
