Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1161 |
| PDBID: | 4ZS7 |
| Chains: | HL_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.93 |
| Reference: | 10.1074/jbc.M115.695528 |
| Antibody | |
| Antibody: | Llama 68F2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-6 |
| Antigen mutation: | No |
| Durg Target: | P05231 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4ZS7_H|Chain B[auth H]|Llama Fab fragment 68F2 heavy chain|Lama glama (9844) EVQLQESGPGLVKPSQTLSLTCTVSGGSITTRYYAWSWIRQPPGKGLEWMGVIDYDGDTYYSPSLKSRTSISWDTSKNQFSLQLSSVTPEDTAVYYCARDPDVVTGFHYDYWGQGTQVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: L
Mutation: NULL
| >4ZS7_L|Chain C[auth L]|Llama Fab fragment 68F2 light chain|Lama glama (9844) QAVLTQPPLVSGTPGQTVTISCAGANNDIGTYAYVSWYQQLPGTAPKLLIYKVTTRASGIPSRFSGSKSGNTASLTISGLQSEDEADYYCASYRNFNNAVFGRGTHLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >4ZS7_A|Chain A|Interleukin-6|Homo sapiens (9606) PHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ARG32 TYR33 ASP54 TYR55 ASP56 GLY57 ASP58 THR59 TYR60 ASP102 VAL103 VAL104 THR105 GLY106 PHE107 L: ASN26 ASN27 GLY30 THR31 TYR32 ALA33 TYR34 LYS52 LYS68 ASN71 TYR93 ASN95 PHE96 ASN97 A: ASP26 ARG30 LEU33 ASP34 SER37 ARG40 LYS66 MET67 GLU69 CYS73 PHE74 GLN75 SER76 HIS164 ARG168 LYS171 GLU172 GLN175 SER176 LEU178 ARG179 ALA180 ARG182 GLN183 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
