Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1152 |
| PDBID: | 4ZFG |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.27 |
| Reference: | 10.1074/jbc.M115.662783 |
| Antibody | |
| Antibody: | 5A12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Angiopoietin-2 |
| Antigen mutation: | No |
| Durg Target: | O15123 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4ZFG_H|Chain B[auth H]|Fragment antigen binding 5A12 heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTISDYWIHWVRQAPGKGLEWVAGITPAGGYTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARFVFFLPYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >4ZFG_L|Chain C[auth L]|Fragment antigen binding 5A12 light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQFLSSFGVAWYQQKPGKAPKLLIYGASSLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGLLSPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >4ZFG_A|Chain A|Angiopoietin-2|Homo sapiens (9606) AGSEQISFRDCAEVFKSGHTTNGIYTLTFPNSTEEIKAYCDMEAGGGGWTIIQRREDGSVDFQRTWKEYKVGFGNPSGEYWLGNEFVSQLTNQQRYVLKIHLKDWEGNEAYSLYEHFYLSSEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDGDNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADFGNSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TRP33 HIS35 TYR58 PHE97 PHE98 LEU99 L: ILE2 PHE27 LEU28 SER29 SER30 PHE31 SER67 GLY68 THR69 GLY91 LEU92 LEU93 SER94 LEU96 A: LYS432 CYS433 ILE434 CYS435 MET440 LEU441 ASP448 ALA449 CYS450 GLY451 PRO452 PHE469 LYS473 TYR475 TYR476 SER480 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
