Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1151 |
| PDBID: | 4ZFF |
| Chains: | AB_D |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.75 |
| Reference: | 10.1074/jbc.M115.662783 |
| Antibody | |
| Antibody: | 5A12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Vascular endothelial growth factor A |
| Antigen mutation: | No |
| Durg Target: | P15692 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4ZFF_A|Chain A, E[auth H]|Fragment antigen binding (Fab) 5A12 Heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTISDYWIHWVRQAPGKGLEWVAGITPAGGYTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARFVFFLPYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: B
Mutation: NULL
| >4ZFF_B|Chain B, F[auth L]|Fragment antigen binding (Fab) 5A12 Light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQFLSSFGVAWYQQKPGKAPKLLIYGASSLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGLLSPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >4ZFF_D|Chain C, D|Vascular endothelial growth factor A|Homo sapiens (9606) HHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER30 ASP31 TYR32 PRO52 PHE97 PHE98 LEU99 B: TYR49 SER53 D: LYS48 MET81 ILE83 PRO85 HIS86 GLN87 GLY88 GLN89 HIS90 ILE91 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
