Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1141 |
| PDBID: | 4Z5R |
| Chains: | UT_I |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1002/pro.2729 |
| Antibody | |
| Antibody: | Rontalizumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Rontalizumab(Phase-II) |
| Antigen | |
| Antigen: | Interferon alpha-2 |
| Antigen mutation: | No |
| Durg Target: | P01563 |
Sequence information
Antibody
Heavy Chain: U
Mutation: NULL
| >4Z5R_U|Chain C[auth B], F[auth K], I[auth M], L[auth Q], O[auth S], R[auth U], U[auth W], X[auth Z]|anti-IFN-a antibody rontalizumab heavy chain modules VH and CH1 (Fab)|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCATSGYTFTEYIIHWVRQAPGKGLEWVASINPDYDITNYNQRFKGRFTISLDKSKRTAYLQMNSLRAEDTAVYYCASWISDFFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: T
Mutation: NULL
| >4Z5R_T|Chain B[auth A], E[auth J], H[auth L], K[auth P], N[auth R], Q[auth T], T[auth V], W[auth Y]|anti-IFN-a antibody rontalizumab light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQSVSTSSYSYMHWYQQKPGKAPKVLISYASNLESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQHSWGIPRTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: I
Mutation: NULL
| >4Z5R_I|Chain A[auth D], D[auth E], G[auth F], J[auth G], M[auth H], P[auth I], S[auth N], V[auth X]|Interferon alpha-2|Homo sapiens (9606) CDLPQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| T: THR27 SER28 SER29 TYR30 TYR32 TYR50 SER52 ASN53 GLU55 SER91 TRP92 GLY93 ILE94 ARG96 U: ILE33 ASP53 TYR54 ILE56 ASN58 LYS64 TRP95 ILE96 SER97 ASP98 PHE99 ASP101 I: ARG12 LEU15 MET16 ALA19 ARG22 SER25 LEU26 PHE27 LEU30 LYS31 ARG33 HIS34 ASP35 PHE36 GLY37 PHE38 GLN40 GLU41 ARG125 GLU141 ARG144 ALA145 MET148 ARG149 SER152 LEU153 ASN156 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
