Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1125 |
| PDBID: | 4YQX |
| Chains: | NO_M |
| Organism: | Rattus norvegicus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.83 |
| Reference: | 10.1016/j.immuni.2015.04.015 |
| Antibody | |
| Antibody: | JES6-1 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: N
Mutation: NULL
| >4YQX_N|Chain A[auth H], D[auth N]|JES6-1 VH domain|Rattus norvegicus (10116) GSEIQLQQSGPELRRPGSSVKLSCKASGYNITDYLIHWVRHRPEHGLEWIGWIDPEDGETRYAQKFQSKATLTADTSSNAAYMQLSSLTPEDTATYFCARSLDSTYIYPFAYWGQGTLVTVSS |
Light Chain: O
Mutation: NULL
| >4YQX_O|Chain B[auth L], E[auth O]|JES6-1 VL domain|Rattus norvegicus (10116) GSDIVMTQSPFSLAVSEGEMVTINCKSSQSLLSSGNQKNYLAWYQQKPGQSPKLLIYYASTGQSGVPDRFIGSGSGTDFTLTISDVQAEDLADYYCLQHYISPPTFGAGTKLELKAAA |
Antigen
Chain: M
Mutation: NULL
| >4YQX_M|Chain C[auth A], F[auth M]|Interleukin-2|Mus musculus (10090) GPGSHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQAAA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: THR33 ASP34 TYR35 LEU36 TRP53 ASP55 GLU57 ASP58 GLU60 ARG62 SER105 THR106 TYR107 ILE108 TYR109 O: SER3 GLN30 SER34 SER35 GLY36 ASN37 TYR41 HIS100 TYR101 ILE102 SER103 PRO104 M: MET33 ASP34 GLN36 GLU37 LEU39 SER40 ARG41 GLU43 ASN44 TYR45 ARG46 ASN47 LYS49 ARG52 MET53 GLN93 ASP96 PHE100 VAL130 ARG134 GLN141 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
