Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1122 |
| PDBID: | 4YPG |
| Chains: | HL_D |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1074/jbc.M115.652156 |
| Antibody | |
| Antibody: | Sifalimumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Sifalimumab(Phase-II) |
| Antigen | |
| Antigen: | Interferon alpha-2 |
| Antigen mutation: | No |
| Durg Target: | P01563 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4YPG_H|Chain B, E[auth H]|Sifalimumab heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYSISWVRQAPGQGLEWMGWISVYNGNTNYAQKFQGRVTMTTDTSTSTAYLELRSLRSDDTAVYYCARDPIAAGYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >4YPG_L|Chain A, F[auth L]|Sifalimumab light chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQSVSSTYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPRTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >4YPG_D|Chain C, D|Interferon alpha-2|Homo sapiens (9606) TSCDLPQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER31 TYR32 SER33 TRP47 TRP50 SER52 TYR54 ASN55 ASN57 THR58 ASN59 ARG98 ASP99 PRO100 ILE101 ALA102 ALA103 TYR105 L: SER28 VAL29 SER30 SER31 THR32 TYR33 TYR50 GLY51 ALA52 SER53 SER54 ARG55 ALA56 THR57 ASP61 GLY67 SER68 GLY69 TYR92 GLY93 SER94 SER95 ARG97 D: THR-1 SER0 ASP2 ILE53 PRO54 HIS57 GLU58 GLN61 GLN62 PHE64 ASN65 SER68 LYS70 THR86 TYR89 GLN90 LEU92 ASN93 GLU96 ALA97 VAL99 ILE100 GLN101 GLY104 VAL105 THR106 PRO109 MET111 GLU113 ILE116 ARG120 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
