Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1102 |
| PDBID: | 4YBQ |
| Chains: | DC_B |
| Organism: | Rattus norvegicus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.27 |
| Reference: | 10.1038/nature14909 |
| Antibody | |
| Antibody: | GLUT5 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | facilitated glucose transporter member 5 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >4YBQ_D|Chain D, F|antibody Fv fragment heavy chain|Mus musculus (10090) LEVNLVESGGGLVQPGGSRKLSCAASGFTFSSFGMHWVRQAPEKGLEWVAHISSGSRTIDYADTVKGRFTISRDNPKNTLFLQMTSLRSEDTAIYYCARGNGYYDALDYWGQGTSVTVSSAKTTPPSVTSENLYFQ |
Light Chain: C
Mutation: NULL
| >4YBQ_C|Chain C, E|antibody Fv fragment light chain|Mus musculus (10090) ELDIVLTQSPLSLPVSLGDQASISCRSSQSIVHSNGNTYLEWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCFQGSHVPYTFGGGTKLEIKTSENLYFQ |
Antigen
Chain: B
Mutation: NULL
| >4YBQ_B|Chain A, B|Solute carrier family 2, facilitated glucose transporter member 5|Rattus norvegicus (10116) MEKEDQEKTGKLTLVLALATFLAAFGSSFQYGYNVAAVNSPSEFMQQFYYDTYYDRNKENIESFTLTLLWSLTVSMFPFGGFIGSLMVGFLVNNLGRKGALLFNNIFSILPAILMGCSKIAKSFEIIIASRLLVGICAGISSNVVPMYLGELAPKNLRGALGVVPQLFITVGILVAQLFGLRSVLASEEGWPILLGLTGVPAGLQLLLLPFFPESPRYLLIQKKNESAAEKALQTLRGWKDVDMEMEEIRKEDEAEKAAGFISVWKLFRMQSLRWQLISTIVLMAGQQLSGVNAIYYYADQIYLSAGVKSNDVQYVTAGTGAVNVFMTMVTVFVVELWGRRNLLLIGFSTCLTACIVLTVALALQNTISWMPYVSIVCVIVYVIGHAVGPSPIPALFITEIFLQSSRPSAYMIGGSVHWLSNFIVGLIFPFIQVGLGPYSFIIFAIICLLTTIYIFMVVPETKGRTFVEINQIFAKKNKVSDVYPEKEEKELNDLPPATREQENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: HIS33 SER34 ASN35 ASN37 TYR39 LYS57 GLY98 TYR103 D: SER31 SER32 GLY34 HIS36 HIS51 SER53 SER54 GLY55 ARG57 THR58 ASN75 GLY102 TYR103 ASP105 B: GLU7 LYS8 THR9 LYS11 VAL15 GLU226 GLU230 LYS231 GLN234 THR235 GLY238 TRP239 LYS240 ASP241 VAL242 ASP243 MET244 MET246 GLU247 ARG250 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
