Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1096 |
| PDBID: | 4Y5X |
| Chains: | ED_F |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.15 |
| Reference: | 10.1016/j.cell.2015.02.011 |
| Antibody | |
| Antibody: | 310 diabody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Erythropoietin receptor |
| Antigen mutation: | No |
| Durg Target: | P19235 |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >4Y5X_E|Chain B, E, H, K|diabody 310 VH domain|Homo sapiens (9606) QSVLTQPPSVSEAPGQRVTIACSGSSSNIGNNAVSWYQQLPGKAPTLLIYYDNLLPSGVSDRFSGSKSGTSASLAISGLQSEDEADYYCAAWDDSLNDWVFGGGTKVTVLAAAGGG |
Light Chain: D
Mutation: NULL
| >4Y5X_D|Chain A, D, G, J|diabody 310 VL domain|Homo sapiens (9606) HSAFAGSEVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCVKDRVAVAGKGSYYFDSWGRGTTVTVSSGGGGS |
Antigen
Chain: F
Mutation: NULL
| >4Y5X_F|Chain C, F, I, L|Erythropoietin receptor|Homo sapiens (9606) FAGSADPKFESKAALLAARGPEELLCFTERLEDLVCFWEEAASAGVGPGQYSFSYQLEDEPWKLCRLHQAPTARGAVRFWCSLPTADTSSFVPLELRVTAASGAPRYHRVIHINEVVLLDAPVGLVARLADESGHVVLRWLPPPETPMTSHIRYEVDVSAGQGAGSVQRVEILEGRTECVLSNLRGRTRYTFAVRARMAEPSFGGFWSAWSEPVSLLTPSDLDKEKAAA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: SER30 SER31 TYR32 SER52 GLY53 SER54 GLY56 SER57 ARG100 VAL101 ALA102 VAL103 LYS106 GLY107 TYR109 TYR110 E: GLY30 ASN31 ASN32 ALA33 TYR50 TYR51 ASP52 ASN53 LEU54 LYS67 F: ARG32 LEU33 GLU34 LEU59 GLU60 ASP61 GLU62 PRO63 LYS65 THR87 ALA88 ASP89 THR90 SER91 SER92 PHE93 VAL94 GLU147 THR148 PRO149 MET150 THR151 SER152 HIS153 PRO203 SER204 PHE205 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
