Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1091 |
| PDBID: | 4Y5V |
| Chains: | AB_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1016/j.cell.2015.02.011 |
| Antibody | |
| Antibody: | 305 diabody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Erythropoietin receptor |
| Antigen mutation: | No |
| Durg Target: | P19235 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4Y5V_A|Chain A, D, G|diabody 305 VH domain|Homo sapiens (9606) HSAFAGSEVQLVESGGGLVQPGGSLRLSCAASGFTFSSYWMSWVRQAPGKGLEWVANIKPDGSEKYYVDSVKGRFTISRDNAKNSVYLQMNSLRAEDTAVYYCARVSRGGSYSDWGQGTLVTVSSGGGGS |
Light Chain: B
Mutation: NULL
| >4Y5V_B|Chain B, E, H|Diabody 305 VL domain|Homo sapiens (9606) SQSALTQPASVSGSPGQSITISCTGTSSDVGGYIYVSWYQQHPGKAPKLMIYDVSRRPSGISDRFSGSKSGNTASLTISGLQAEDEADYYCNSYTTLSTWLFGGGTKVTVLAAAGGG |
Antigen
Chain: C
Mutation: NULL
| >4Y5V_C|Chain C, F, I|Erythropoietin receptor|Homo sapiens (9606) FAGSADPKFESKAALLAARGPEELLCFTERLEDLVCFWEEAASAGVGPGQYSFSYQLEDEPWKLCRLHQAPTARGAVRFWCSLPTADTSSFVPLELRVTAASGAPRYHRVIHINEVVLLDAPVGLVARLADESGHVVLRWLPPPETPMTSHIRYEVDVSAGQGAGSVQRVEILEGRTECVLSNLRGRTRYTFAVRARMAEPSFGGFWSAWSEPVSLLTPSDLDKEKAAA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: PHE27 THR28 SER31 TYR32 TRP33 LYS52 PRO53 GLU57 TYR59 ARG98 VAL99 SER100 ARG101 GLY102 GLY103 B: SER26 SER27 GLY31 TYR32 ILE33 TYR34 ASP52 ARG55 SER58 TYR93 THR95 SER97 TRP99 C: ARG32 LEU33 GLU34 LEU59 GLU60 ASP61 GLU62 THR87 ALA88 ASP89 THR90 SER91 SER92 PHE93 VAL94 PRO95 HIS114 ASN116 GLU117 PRO149 MET150 SER152 HIS153 GLU202 PRO203 SER204 PHE205 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
