Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1090 |
| PDBID: | 4XZU |
| Chains: | AB_M |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.61 |
| Reference: | 10.1038/srep17216 |
| Antibody | |
| Antibody: | 3E6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Coagulation factor VIII |
| Antigen mutation: | No |
| Durg Target: | P00451 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4XZU_A|Chain A, D[auth E]|3E6 antibody Fab heavy chain|Mus musculus (10090) QIQLVQSGPELKKPGKTVKISCKASDYTFTDYSLHWVKQAPGKGLKWMGWINTETGDPAYADDFKGRFAFSLETSVRTAYLQINNLKNEDTAIYFCAREDDGLASWGQGTTLTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPA |
Light Chain: B
Mutation: NULL
| >4XZU_B|Chain B, E[auth F]|3E6 antibody Fab light chain|Mus musculus (10090) QIVLTQSPAIMSASPGEKVTMTCSASSTVSYMYWYQQKPGSSPRFLISDTSNLASGVPVRFSGSGSGTSYSLTISRIEAEDAATYYCQHWSSYPLTFGGGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHSTSTSPIVKSFNRNEC |
Antigen
Chain: M
Mutation: NULL
| >4XZU_M|Chain C[auth G], F[auth M]|Coagulation factor VIII|Homo sapiens (9606) CSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISSSQDGHQWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR30 ASP31 TYR32 SER33 TRP50 ASN52 THR53 GLU54 THR55 ASP57 ALA59 GLU99 ASP100 ASP101 B: GLN1 ILE2 THR28 SER30 TYR31 TRP90 SER91 SER92 TYR93 M: GLU2181 SER2182 LYS2183 ALA2184 SER2186 ASP2187 ALA2188 THR2202 SER2204 SER2206 LYS2207 HIS2211 LEU2212 GLN2213 GLY2214 ARG2215 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
