Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 109 |
| PDBID: | 1NCB |
| Chains: | HL_N |
| Organism: | Influenza A virus (A/tern/Australia/G70C/1975(H11N9)), Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1016/0022-2836(92)90688-g |
| Antibody | |
| Antibody: | NC41 Fab |
| Antibody mutation: | Yes |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus subtype N9 neuraminidase |
| Antigen mutation: | No |
| Durg Target: | P03472 |
Sequence information
Antibody
Heavy Chain: H
Mutation: K46E/T91K/V116L
| >1NCB_H|Chain C[auth H]|IGG2A-KAPPA NC41 FAB (HEAVY CHAIN)|Mus musculus (10090) QIQLVQSGPELKKPGETVKISCKASGYTFTNYGMNWVKQAPGKGLEWMGWINTNTGEPTYGEEFKGRFAFSLETSASTANLQINNLKNEDKATFFCARGEDNFGSLSDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRG |
Light Chain: L
Mutation: NULL
| >1NCB_L|Chain B[auth L]|IGG2A-KAPPA NC41 FAB (LIGHT CHAIN)|Mus musculus (10090) DIVMTQSPKFMSTSVGDRVTITCKASQDVSTAVVWYQQKPGQSPKLLIYWASTRHIGVPDRFAGSGSGTDYTLTISSVQAEDLALYYCQQHYSPPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: N
Mutation: NULL
| >1NCB_N|Chain A[auth N]|INFLUENZA A SUBTYPE N9 NEURAMINIDASE|Influenza A virus (384509) IRDFNNLTKGLCTINSWHIYGKDNAVRIGEDSDVLVTREPYVSCDPDECRFYALSQGTTIRGKHSNGTIHDRSQYRALISWPLSSPPTVYNSRVECIGWSSTSCHDGKTRMSICISGPNNNASAVIWYNRRPVTEINTWARNILRTQESECVCHNGVCPVVFTDGSATGPAETRIYYFKEGKILKWEPLAGTAKHIEECSCYGERAEITCTCRDNWQGSNRPVIRIDPVAMTHTSQYICSPVLTDNPRPDDPTVGKCNDPYPGNNNNGVKGFSYLDGVNTWLGRTISIASRSGYEMLKVPNALTDDKSKPTQGQTIVLNTDWSGYSGSFMDYWAEGECYRACFYVELIRGRPKEDKVWWTSNSIVSMCSSTEFLGQWDWPDGAKIEYFL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: THR31 TYR49 TRP50 SER52 THR53 ARG54 HIS55 ILE56 HIS91 TYR92 SER93 PRO94 TRP96 H: THR30 ASN31 TYR32 GLY33 TRP50 THR52 ASN53 GLU56 LYS64 GLU96 ASP97 ASN98 PHE99 N: ILE149 PRO326 ARG327 PRO328 ASP329 GLY343 ASN344 ASN345 ASN347 ILE366 SER367 ILE368 ALA369 SER370 SER372 LEU399 ASN400 THR401 ASP402 TRP403 PRO431 LYS432 GLU433 ASP434 ASP457 LYS463 GLU465 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
