Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1088 |
| PDBID: | 4XX1 |
| Chains: | MO_J |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.60 |
| Reference: | 10.1194/jlr.M059873 |
| Antibody | |
| Antibody: | Fab1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | LCAT |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >4XX1_M|Chain B[auth E], E[auth H], H[auth M]|Fab1 heavy chain|Homo sapiens (9606) QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAVIWYDGSNKFYEDSVKGRFTISRDNSKNTLYLQMDSLRAEDTAVYYCAREGAAVRSFYYSYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSHSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCAAAENLYFQ |
Light Chain: O
Mutation: NULL
| >4XX1_O|Chain A[auth G], D[auth L], G[auth O]|Fab1 light chain|Homo sapiens (9606) SYELTQPPSVSVSPGQTASITCSGDKLGNKFTSWYQRKPGQSPVLVIYQDTKRPSGIPERFSGSTSGNTATLTISGTQAMDEADYYCQAWDSSTAWVFGGGTKLEVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: J
Mutation: NULL
| >4XX1_J|Chain C[auth A], F[auth B], I[auth J]|Phosphatidylcholine-sterol acyltransferase|Homo sapiens (9606) FWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPEENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| O: ASN29 PHE31 TRP90 ASP91 SER92 THR94 TRP96 M: TRP52 TYR53 SER56 ASN57 PHE59 VAL103 ARG104 SER105 PHE106 TYR107 TYR108 SER109 TYR111 J: LEU40 TRP48 MET49 CYS50 TYR51 ARG52 LYS53 THR54 PHE58 THR59 ILE60 ASP63 ASN65 MET66 LEU68 PRO69 LEU70 GLY71 CYS74 TRP75 ASN78 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
