Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1072 |
| PDBID: | 4XVT |
| Chains: | HL_G |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.69 |
| Reference: | 10.1016/j.cell.2015.03.004 |
| Antibody | |
| Antibody: | VRC07_1995 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade A/E gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4XVT_H|Chain B[auth H]|donor 45 45-VRC01.H01+07.O-863513/45-VRC01.L01+07.O-110653 (VRC07_1995) Heavy chain|Homo sapiens (9606) QVQLLQSGAQVKKTGASMRISCKTSGYTFLNCPINWVRQAPGRGLEWMGWMKPRGGAVNYPQKFQGRVTMTRDMSTDTAFLDMSNLRSDDTAVYFCARGKYCTASDYYNWDFEHWGRGTLVTVSSPATKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >4XVT_L|Chain C[auth L]|45-VRC01.H01+07.O-863513/45-VRC01.L01+07.O-110653 (VRC07_1995) Light chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRTSQYGSLAWYQQRPGQAPRLVIYGGSSRATGIPDRFTGSRSGADYTLTINRLEPEDFGIYYCQQYEFFGQGTKVEVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLASPVTKSFNRGEC |
Antigen
Chain: G
Mutation: NULL
| >4XVT_G|Chain A[auth G]|ENVELOPE GLYCOPROTEIN GP120 OF HIV-1 CLADE A/E|Human immunodeficiency virus 1 (11676) VWKDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVEQMQEDVISLWDQSLQPCVKLTGGSVIKQACPKISFDPIPIHYCTPAGYVILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHLNKSVEINCTRPSNGGSGSGGDIRKAYCEINGTKWNKVLKQVTEKLKEHFNNKTIIFQPPSGGDLEITMHHFNCRGEFFYCNTTQLFNNTCIGNETMKGCNGTITLPCKIKQIINMWQGTGQAMYAPPIDGKINCVSNITGILLTRDGGANNTSNETFRPGGGNIKDNWRSELYKYKVVQIE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: LEU30 PRO33 TRP47 TRP50 LYS52 ARG54 GLY55 GLY56 ALA57 VAL58 ASN59 TYR60 PRO61 GLN62 GLN65 ARG72 MET74 TYR101 ALA104 SER105 ASP106 TYR107 TYR108 ASN109 TRP110 L: VAL5 GLN29 TYR30 TYR91 GLU96 PHE97 G: TRP96 LYS97 GLU102 GLU275 ASN276 THR278 ASN279 ASN280 ALA281 LYS282 THR283 GLN362 SER365 GLY366 GLY367 ASP368 ILE371 TRP427 GLY429 THR430 THR455 ARG456 ASP457 GLY458 GLY459 ALA460 ASN461 ASN462 THR463 ASN465 THR467 ARG469 GLY472 GLY473 ASN474 LYS476 ARG480 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
