Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1068 |
| PDBID: | 4XRC |
| Chains: | BA_D |
| Organism: | Homo sapiens, Influenza A virus |
| Method: | XRD |
| Resolution (Å): | 2.74 |
| Reference: | 10.1073/pnas.1502762112 |
| Antibody | |
| Antibody: | H5.3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H5N1 hemagglutinin |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >4XRC_B|Chain B, E[auth H]|H5.3 Fab heavy chain|Homo sapiens (9606) QVQLQESGPGLVKPSGTVSLTCAVSGGSISSSYWWSWVRQPPGKGLEWIGEIYHSGNTNYNPSLKSRVTISVDKSKNLFSLKLSSVTAADTAVYYCARVALFDILTGGWFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVELKTPT |
Light Chain: A
Mutation: NULL
| >4XRC_A|Chain A, D[auth L]|H5.3 Fab light chain|Homo sapiens (9606) YELTQPPSVSVSPGQTVNITCSGDTLGDKYVCWYQQKPGQSPVLVIYQDTKRPSGIPERFSGSNSGDTATLTVSGTQAMDEADYYCQAWDSSSFVFGTGTKVTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT |
Antigen
Chain: D
Mutation: T109A/Q175L/G177S
| >4XRC_D|Chain C, F[auth D]|Hemagglutinin|Influenza A virus (11320) KPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKANPVNDLCYPGDFNDYEELKHLLSRINHFEKIQIIPKSSWSSHEASLGVSSACPYQGKSSFFRNVVWLIKKNSAYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYISVGTSTLNQRLVPRIATRSKVNGLSSRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR25 GLY27 ASP28 LYS29 TYR30 ASP49 THR50 LYS51 ASN64 GLY66 TRP89 PHE94 B: TRP34 GLU51 TYR53 SER55 ASN57 ASN59 LEU101 PHE102 ASP103 ILE104 LEU105 THR106 GLY107 D: TYR98 LEU133 GLY134 VAL135 SER136 SER137 PRO140 GLN142 GLY143 LYS144 SER145 TRP153 ILE155 LYS156 ASN158 SER159 ALA189 GLU190 LYS193 LEU194 GLN196 LYS222 GLY225 LEU226 SER227 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
