Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1066 |
| PDBID: | 4XPT |
| Chains: | HL_A |
| Organism: | Drosophila melanogaster, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.36 |
| Reference: | 10.1038/nature14431 |
| Antibody | |
| Antibody: | 9D5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dopamine transporter |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4XPT_H|Chain C[auth H]|antibody fragment heavy chain|Mus musculus (10090) EVQLVESGGGLVKPGGSLKLSCAASGFTFSSYAMSWVRQSPEKRLEWVAEISSGGRYIYYSDTVTGRFTISRDNARNILHLEMSSLRSEDTAMYYCARGEVRQRGFDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR |
Light Chain: L
Mutation: NULL
| >4XPT_L|Chain B[auth L]|antibody fragment light chain|Mus musculus (10090) ENVLTQSPAIMSTSPGEKVTMTCRASSSVGSSYLHWYQQKSGASPKLWIYSTSNLASGVPARFSGSGSGTSYSLTISSVEAEDAATYYCQQFSGYPLTFGSGTKLEMKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: A
Mutation: V50A/D97G/G351A/P362M
| >4XPT_A|Chain A|Dopamine transporter|Drosophila melanogaster (7227) DERETWSGKVDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYGIMLAVGGIPLFYMELALGQHNRKGAITCWGRLVPLFKGIGYAVVLIAFYVGFYYNVIIAWSLRFFFASFTNSLPWTSCNNIWNTPNCRPFEVGHVEGFQSAASEYFNRYILELNRSEGIHDLGAIKWDMALCLLIVYLICYFSLWKGISTSGKVVWFTALFPYAVLLILLIRGLTLPGSFLGIQYYLTPNFSAIYKAEVWVDAATQVFFSLGPGFGVLLAYASYNKYHNNVYKDALLTSFINSATSFIAGFVIFSVLGYMAHTLGVRIEDVATEGPGLVFVVYPAAIATMPASTFWALIFFMMLATLGLDSSFGGMEAIITALSDEFPKIKRNRELFVAGLFSLYFVVGLASCTQGGFYFFHLLDRYAAGYSILVAVFFEAIAVSWIYGTNRFSEDIRDMIGFPPGRYWQVCWRFVAPIFLLFITVYGLIGYEPLTYADYVYPSWANALGWCIAGSSVVMIPAVAIFKLLSTPGSLRQRFTILTTPWRD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLU1 PRO8 ALA9 ILE10 SER101 GLY102 PRO142 ASP144 ILE145 ASN146 VAL147 LYS148 TRP149 GLU155 GLN157 ASN158 GLY159 VAL160 ASN162 SER163 TRP164 THR198 LYS200 H: PRO41 GLU42 LYS43 ARG44 PRO173 VAL175 GLN177 A: CYS148 ASN149 ASN150 ILE151 TRP152 ASN153 THR154 PRO155 ARG158 VAL202 VAL205 GLU206 GLY207 PHE208 ARG218 TYR219 ASN224 ARG225 GLU227 ARG377 ASP549 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
