Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1020 |
| PDBID: | 4WEU |
| Chains: | D_B |
| Organism: | Lama glama, Escherichia coli |
| Method: | XRD |
| Resolution (Å): | 2.61 |
| Reference: | 10.1186/s13567-015-0151-x |
| Antibody | |
| Antibody: | Anti-F4+ETEC bacteria VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | K88 fimbrial protein AD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >4WEU_D|Chain A[auth D], D[auth E]|Anti-F4+ETEC bacteria VHH variable region|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGLTFDTYAMGWFRQAPGKKREYVAAISWTGISTYYADIAKGRFTISRDNAKNTLYLQMDSLKPEDTAVYYCAAQKSLNVPAPWDYWGQGTQVTVSSHHHHHH |
Antigen
Chain: B
Mutation: NULL
| >4WEU_B|Chain B[auth A], C[auth B]|K88 fimbrial protein AD|Escherichia coli (562) WMTGHHHHHHDDYRQKWEWKVGTGLNGFGNVLNDLTNGGTKLTITVTGNKPILLGRTKEAFATPVTSGVDGIPHIAFTDYEGASVELRNPDGETEKGLAYFVLPMKNAEGTKVGSVKVNASYAGALGRGGVTSADGELMSLFAEGSHAIFYGGLPTNVKNSELKGGSAAAARTELFGSLSKNDILGQIQRVNANITSLVNVPGSFNENMAYTDGSVVSVAYALGIANGQTIEATFNQAVTTSTQWSAPLNVAITYYDNKQMTGDFNGSVDIGGSITA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: ALA833 TYR847 SER852 TRP853 THR854 ILE856 SER857 TYR859 SER901 LEU902 ASN903 VAL904 PRO905 ALA906 B: THR86 ASP87 TYR88 GLU89 GLY90 LYS114 ALA116 GLU117 GLY118 THR119 LYS120 PRO256 ASN258 SER276 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
