Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1016 |
| PDBID: | 4W6Y |
| Chains: | B_A |
| Organism: | Escherichia coli DH5[alpha], Lama glama |
| Method: | XRD |
| Resolution (Å): | 1.57 |
| Reference: | 10.1371/journal.pone.0114691 |
| Antibody | |
| Antibody: | NbFedF9 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | F18 fimbrial adhesin AC |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >4W6Y_B|Chain B|Nanobody NbFedF9|Lama glama (9844) QVQLQESGGGSVQAGGSLRLSCAASGYTYSSNCMAWFRQVPGKEREGVASINTRGGITYYADSVKGRFTISRDNAKNTVSLQMNSLKPEDTATYYCAAVREATYSDNRCSVRSYTYDYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >4W6Y_A|Chain A|F18 fimbrial adhesin AC|Escherichia coli DH5[alpha] (668369) NSSASSAQVTGTLLGTGKTNTTQMPALYTWQHQIYNVNFIPSSSGTLTCQAGTILVWKNGRETQYALECRVSIHHSSGSINESQWGQQSQVGFGTACGNKKCRFTGFEISLRIPPNAQTYPLSSGDLKGSFSLTNKEVNWSASIYVPAIAK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: CYS33 GLU44 ASN52 TYR59 VAL99 ARG100 GLU101 ALA102 THR103 TYR104 SER105 ASN107 ARG108 CYS109 SER110 ARG112 TYR114 THR115 A: GLN37 GLN45 GLN47 TYR49 HIS88 HIS89 SER90 SER91 GLY92 SER93 ILE94 ASN95 GLU96 ALA110 CYS111 GLY112 LYS114 ARG117 PHE118 GLY120 PHE121 GLU122 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
