On the predict page, you can submit some sequences for prediction. Please Pay attention: the AGOPredict tool only supports protein sequence in FASTA format. Line breaks should be carried out between each sequence, and there should be no blank characters such as spaces or line breaks within each sequence. If you only have raw sequence information, you must construct a simple FASTA format for the raw sequence before making prediction. The format is as follows: ">+name+\n+sequence", where the "name" is the name you specify for the sequence and it can be made up of any non-null characters, as shown in the example. If you upload a sequence file, please name the file suffix as .txt or .fasta format. Besides, only the standard IUPAC one-letter codes for the amino acids (A,C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y) are supported.
Furthermore, the threshold to distinguish between predicted positives and negatives (tp) ranges from 0 to 1. However, it is set to 0.5 by default. That is to say, a sequence will be predicted to be a Cas protein if the probability is 0.5 or higher. You can adjust the threshold according to your own needs. Here we go!