General information
    ACovPid:ACoVP100444
    Trivial Name:SAP2
    Amino Acids Sequence:EDLFYQSSL
    Length:9
    C-Terminal Modification:None
    N-Terminal Modification:None
    Chemical Modification:None
    Peptide Source:

    Human Angiotensin Converting Enzyme 2 (hACE2) : 9606

    Source Description:
    Against Virus:

    Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) : 2697049

    Inhibition Value Type:IC50
    Inhibitory Effect:3.72 ± 0.37
    Inhibitory Unit:µM
    Target Domain Name:RBD of SARS-CoV-2
    Assay:Pseudotyped virus infection inhibition assay
    Assay Description:HEK293T-ACE2 cells were seeded in µClear Black 96-well plates (Greiner Bio-One) in 100 µL of DMEM supplemented with 10% FBS at a density of 1.25 × 10^4 cells per well. Sixteen hours after plating, equal amounts (RLUs/mL) of a given pseudovirus were incubated with indicated concentrations of the test peptide or diluent control (1× PBS) in 50 µL of DMEM supplemented with 10% FBS for 1 h at 37 °C in a V-bottom 96-well plate. The virus–peptide mixture was then added to the HEK293T-ACE2 cells. Polybrene (Sigma) at the final concentration of 5 µg/mL was added to the cells. After 48 h, 100 µL of supernatant was removed from each well and luciferase activity was measured using Bright-Glo Luciferase Assay System (Promega) following the manufacturer’s protocol. Luminescence was detected using an Infinite M PLEX multimode plate reader (Tecan).
    Anti-CoV activity in vivo:
    Reference:33356169
    Comment:Pseudovirus Production:Luciferase-encoding lentiviruses pseudotyped with viral glycoprotein of interest were generated using methods described previously.47 Briefly, HEK293T cells were transfected with pNL4–3.Luc.R–E– and pCAGGS-SARS-CoV-2 Spike, pCAGGS-SARS-CoV Spike, or pMD2.G using Fugene 6 transfection reagent (Roche) following manufacturer’s protocol. Forty-eight hours post-transfection, virus-containing supernatants were harvested, filtered through 0.45 µm sterile filter, and concentrated using Amicon Ultra-15 centrifugal filters (Millipore). Aliquots of pseudoviruses were stored at −80 °C. The titers of SARS-CoV-2 Spike-, SARS-CoV Spike-, and VSV-G-pseudotyped viruses were in the range of ∼2 × 105, ∼1 × 105, and ∼3 × 107 relative luciferase units (RLUs)/mL, respectively.
    3D structure:

    StructureACoVP100444

    Structure Experiment Verified:NO
    Similar Peptides:ACoVP100432   ACoVP100431   ACoVP100429   ACoVP100150   ACoVP100495

    Target Domain information
    Target Domain Full Name:Receptor-binding domain (RBD) of Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) spike glycoprotein
    Target Type:glycoprotein
    UniprotID [Sequence]:

    P0DTC2 [319-541]

    Target Synonyms:Alternative name(s) for spike glycoprotein: E2 Peplomer protein S glycoprotein
    Target Source:

    Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) : 2697049

    Target Structure:6LVN, 6LXT, 6LZG, 6M0J, 6M17, 6M1V, 6VSB, 6VW1, 6VXX, 6VYB, 6W41, 6WPS, 6WPT, 6X29, 6X2A, 6X2B, 6X2C, 6X6P, 6X79, 6XC2, 6XC3, 6XC4, 6XC7, 6XCM, 6XCN, 6XDG, 6XE1, 6XEY, 6XF5, 6XF6, 6XKL, 6XKP, 6XKQ, 6XLU, 6XM0, 6XM3, 6XM4, 6XM5, 6XR8, 6XRA, 6XS6, 6YLA, 6YM0, 6YOR, 6YZ5, 6YZ7, 6Z2M, 6Z43, 6Z97, 6ZB4, 6ZB5, 6ZBP, 6ZCZ, 6ZDG, 6ZDH, 6ZER, 6ZFO, 6ZGE, 6ZGG, 6ZGI, 6ZH9, 6ZHD, 6ZLR, 6ZOW, 6ZOX, 6ZOY, 6ZOZ, 6ZP0, 6ZP1, 6ZP2, 6ZP5, 6ZP7, 6ZWV, 6ZXN, 7A25, 7A29, 7A4N, 7A5R, 7A5S, 7A91, 7A92, 7A93, 7A94, 7A95, 7A96, 7A97, 7A98, 7AD1, 7BWJ, 7BYR, 7BZ5, 7C01, 7C2L, 7C8D, 7C8V, 7C8W, 7CAB, 7CAH, 7CAI, 7CAK, 7CAN, 7CDI, 7CDJ, 7CH4, 7CH5, 7CHB, 7CHC, 7CHE, 7CHF, 7CHH, 7CJF, 7CN9, 7CT5, 7CWM, 7CWN, 7CWO, 7CWS, 7CWU, 7DCC, 7DCX, 7DD2, 7DD8, 7DDD, 7DDN, 7DF3, 7DF4, 7DK3, 7DK4, 7DK5, 7DK6, 7DK7, 7DMU, 7JJC, 7JJI, 7JJJ, 7JMO, 7JMP, 7JMW, 7JV2, 7JV4, 7JV6, 7JVA, 7JVB, 7JVC, 7JW0, 7JWB, 7JWY, 7JX3, 7JZL, 7JZM, 7JZN, 7JZU, 7K43, 7K45, 7K4N, 7K8M, 7K8S, 7K8T, 7K8U, 7K8V, 7K8W, 7K8X, 7K8Y, 7K8Z, 7K90, 7K9Z, 7KDG, 7KDH, 7KDI, 7KDJ, 7KDK, 7KDL, 7KE4, 7KE6, 7KE7, 7KE8, 7KE9, 7KEA, 7KEB, 7KEC, 7KJ2, 7KJ3, 7KJ4, 7KJ5, 7KKK, 7KKL, 7KL9, 7KMB, 7KMS, 7KMZ, 7KNB, 7KNE, 7KNH, 7KNI, 7L02, 7L06, 7L09