General information
    ACovPid:ACoVP100403
    Trivial Name:Peptide #5
    Amino Acids Sequence:DISGINASVVNIQKEIDRLNEVAKNLNESLIGLQEL
    Length:36
    C-Terminal Modification:None
    N-Terminal Modification:None
    Chemical Modification:None
    Peptide Source:

    Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) / Severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) / Middle East respiratory syndrome coronavirus (MERS-CoV) : 694009/2697049

    Source Description:From the heptad repeat region 2 of spike protein
    Against Virus:

    Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) : 2697049

    Inhibition Value Type:IC50
    Inhibitory Effect:4.7
    Inhibitory Unit:µM
    Target Domain Name:
    Assay:DSP assay DSP assay for SARS-CoV-2 S-mediated cell-cell fusion
    Assay Description:The DSP assay using 293FT cells was performed as described previously (PMID: 32532094) to monitor SARS-CoV-2-S-mediated membrane fusion. Briefly, effector cells expressing SARS-CoV-2-S protein with DSP8-11, target cells expressing ACE2, and transmembrane serine protease 2 (TMPRSS2) with DSP1-7 were seeded in 10 cm culture dishes (4×10^6 cells/10 mL) one day before the assay. Two hours before the DSP assay, cells were treated with 6 µM EnduRen (Promega, Madison, WI, USA), a substrate for Renilla luciferase, to activate EnduRen. One microliter of each peptide dissolved in dimethyl sulfoxide (DMSO) was added to the 384-well plates (Greiner Bioscience, Frickenhausen, Germany). Next, 50 µL of each single-cell suspension (effector and target cells) was added to the wells using a Multidrop dispenser (Thermo Fisher Scientific, Waltham, MA, USA). After incubation at 37°C for 4 h, luciferase activity was measured using a Centro xS960 luminometer (Berthold, Bad-Wildbad, Germany).
    Anti-CoV activity in vivo:
    Reference:33424013
    Comment:The concluded sequence for peptide synthesis was generated after alignment of SARS-CoV, SARS-CoV-2, and MERS-CoV HR2 sequences.
    3D structure:

    StructureACoVP100403

    Structure Experiment Verified:NO
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