General information
    ACovPid:ACoVP100389
    Trivial Name:HR2-44
    Amino Acids Sequence:GDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYI
    Length:44
    C-Terminal Modification:None
    N-Terminal Modification:None
    Chemical Modification:None
    Peptide Source:

    Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009

    Source Description:From the heptad repeat region 2 of spike protein of SARS-CoV
    Against Virus:

    Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009

    Inhibition Value Type:IC50
    Inhibitory Effect:0.5
    Inhibitory Unit:µM
    Target Domain Name:
    Assay:Cell–cell fusion assay
    Assay Description:Our simple and convenient cell fusion inhibition assay can be used to screen and identify entry inhibitors of SARS-CoV. The requirement of trypsin treatment in this assay suggests that it mimics the entry from cell surface by direct fusion between viral and cell membranes. To examine the inhibitory effect of the peptides identified by the cell fusion inhibition assay on SARS-CoV, we carried out a previously described SARS-CoV infectivity assay for three HR1 peptides at the BSL-3 facility (PMID: 14519257, 15606632). Briefly, VeroE6 cells (1 × 10^4 cells, each well) prepared in 96-well were infected with 75 TCID50 of SARS-CoV TW01 strain, which were pre-incubated with or without different concentration of peptides in quadricates at 37 °C for 1 h. After incubation at 37 °C for 72 h, cytopathic effects were observed under light microscope, and the IC50 was determined.
    Anti-CoV activity in vivo:
    Reference:15158473
    Comment:
    3D structure:

    StructureACoVP100389

    Structure Experiment Verified:NO
    Similar Peptides:ACoVP100125   ACoVP100119   ACoVP100120   ACoVP100118   ACoVP100121