| General information | |
| ACovPid: | ACoVP100372 |
| Trivial Name: | K12 |
| Amino Acids Sequence: | GGASCCLYCRCH |
| Length: | 12 |
| C-Terminal Modification: | None |
| N-Terminal Modification: | None |
| Chemical Modification: | None |
| Peptide Source: | Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009 |
| Source Description: | Sequence of nsp 10 of SARS-CoV |
| Against Virus: | Severe acute respiratory syndrome coronavirus (SARS-CoV) : 694009 |
| Inhibition Value Type: | IC50 |
| Inhibitory Effect: | 160 |
| Inhibitory Unit: | µM |
| Target Domain Name: | |
| Assay: | SAM binding assays |
| Assay Description: | 25 µl reaction mixtures [40 mM Tris–HCl (pH 7.5), 2 mM MgCl2, 2 mM DTT] containing 0.5 µM purified nsp16, different concentrations of nsp10 or its truncations and 2 µCi of S-adenosyl [methyl-3H] methionine (67.3 Ci/mmol, 0.5 µCi/µl) were pipetted into wells of a microtiter plate. The reaction mixtures were incubated on ice and irradiated with 254-nm UV light in a Hoefer UVC500 cross-linking oven for 30 min. The distance of samples from the UV tubes was 4 cm. The samples were then analyzed by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The gels were socked in Enlightening buffer (PerkinElmer) and analyzed by autoradiography |
| Anti-CoV activity in vivo: | |
| Reference: | 22659295 |
| Comment: | |
| 3D structure: | |
| Structure Experiment Verified: | NO |
| Similar Peptides: | ACoVP100373 ACoVP100437 |