2351 LGDSSNSQVSLN(1) LPHSAVMAQLTY(1) STSHHHHPSAPS(1) TLHAHQHHQPST(1) WMNGPVSIRTWS(1) WPYNHHHRTPSP(1) YSHHHMHTPHTR(1) 7 Phage display (subtractive panning) 2 PMID:24287902 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The phage display peptide library was incubated with Ni-agarose magnetic beads in microtubes to eliminate phages binding to the beads or the tubes. In the first round, 120 μg of GB-His in immobilization buffer was added to 120 μ L of Ni-agarose magnetic.The library was subsequently added to 120 μg of GB-His immobilized Ni-agarose magnetic beads. In the second round, the first-round-eluted phages were incubated with 48 μg of GB-His. In the third round (negative-selection round), the phages were incubated with DEE-His. 2352 APLSQHHHHLRP(1) ELITNSETTQWF(1) MHDLTAALSLPP(1) TMQPGQNSHPIL(1) TSHIHTTPHSHH(1) YHPANHSFQHHF(1) YNHHGHHLDKHR(1) 7 Phage display (subtractive panning) 2 PMID:24287902 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The phage display peptide library was incubated with Ni-agarose magnetic beads in microtubes to eliminate phages binding to the beads or the tubes. In the first round, 120 μg of GB-His in immobilization buffer was added to 120 μ L of Ni-agarose magnetic.The library was subsequently added to 120 μg of GB-His immobilized Ni-agarose magnetic beads. In the second round, the first-round-eluted phages were incubated with 48 μg of GB-His. In the third round (negative-selection round), the phages were incubated with HI. 2353 SDLSPIQSLSAI(2) APLFTQTWGPWR(1) APYAHHHHPVTP(1) EELWHHHPPSHH(1) LGDSSNSQVSLN(1) NSTHHHHFATIW(1) QPHKYPHSHHGP(1) TSLHQHHPTAAF(1) VAQHSHHHVTPS(1) 9 Phage display (subtractive panning) 3 PMID:24287902 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The phage display peptide library was incubated with Ni-agarose magnetic beads in microtubes to eliminate phages binding to the beads or the tubes. In the first round, 120 μg of GB-His in immobilization buffer was added to 120 μ L of Ni-agarose magnetic.The library was subsequently added to 120 μg of GB-His immobilized Ni-agarose magnetic beads. In the second round, the first-round-eluted phages were incubated with 48 μg of GB-His. In the third round (negative-selection round), the phages were incubated with DEE-His. In the forth round, the third-round-eluted phages were screened against 2-μg wild-type GDH-B immobilized in a well of a 96-well ELISA plate. 2354 SPHSHHMSPSEY(2) LSPHHHHLDGHI(2) ANPLHHHHLWEL(1) NSTHHHHFATIW(1) QPHKQAVSFAFA(1) QTSHYHHHRAHT(1) TPTFPYWYGSLT(1) VHTHHLGHQPVR(1) 8 Phage display (subtractive panning) 3 PMID:24287902 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The phage display peptide library was incubated with Ni-agarose magnetic beads in microtubes to eliminate phages binding to the beads or the tubes. In the first round, 120 μg of GB-His in immobilization buffer was added to 120 μ L of Ni-agarose magnetic.The library was subsequently added to 120 μg of GB-His immobilized Ni-agarose magnetic beads. In the second round, the first-round-eluted phages were incubated with 48 μg of GB-His. In the third round (negative-selection round), the phages were incubated with HI. In the forth round, the third-round-eluted phages were screened against 2-μg wild-type GDH-B immobilized in a well of a 96-well ELISA plate. 2355 CDRRDLPDWAIRAC 1 Phage display (common panning) 4 PMID:24304511 HIV-1 CDC451 gp120 Not determined. Not determined. Not determined. CX12C phage display library The peptide, CDRRDLPDWAIRAC, not only bound to gp120 but also enabled the binding of the stringent CD4i mAb CG10. 2356 LYQDYSL(2) EPLQLKM(2) LPWKPLG(2) TPAHPNY(2) LGAQSNF(1) PGAQSNF(1) VNSPTHS(1) VNSATHS(1) YQDSAKT(1) 9 Phage display (in vivo) 3-4 PMID:24320790 Four- to five-week-old mdx mice heart Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Four- to five-week-old mdx mice were injected intravenously with phages (one animal per round). Mice were perfused with phosphate-buffered saline (PBS) after 1 hour to remove unbound phages. The gastrocnemius and heart muscles were isolated and washed three times with Tris-buffered saline (TBS). Subsequently the organs were homogenized in 2 mL TBS with MagNA Lyser green beads (Roche Diagnostics) in the MagNA Lyser (Roche Diagnostics). The suspension was titrated and amplified. Up to four rounds of biopanning were performed for each fraction. After the third and fourth round, phages were plated and colonies were picked. The selected peptides were synthesized with a FAM label and incubated with primary human control myotube cultures and analyzed with fluorescence microscopy. Clear fluorescence was observed for 7 of the 12 peptides. Based on the highest fluorescence intensity, P1 (LYQDYSL), P2 (LPWKPLG) and P4 (LGAQSNF) were chosen for in vivo evaluation. Mice were intramuscularly injected with labeled peptide in the gastrocnemius muscle. Analyzed with fluorescence microscopy, clear fluorescence was observed for P4 at the membrane. P4 was conjugated to a 2OMePS AON specific for mouse dystrophin exon 23 (23AON). Upon systemic administration in dystrophic mdx mice, conjugation of a 2'-O-methyl phosphorothioate AON to this peptide indeed improved uptake in skeletal and cardiac muscle, and resulted in higher exon skipping levels with a significant difference in heart and diaphragm. 2357 EPLQLKM(2) TALPPSY(2) AMISAIH(1) HVIANAG(1) GNTPSRA(1) 5 Phage display (in vivo) 3-4 PMID:24320790 Four- to five-week-old mdx mice muscle Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Four- to five-week-old mdx mice were injected intravenously with phages (one animal per round). Mice were perfused with phosphate-buffered saline (PBS) after 1 hour to remove unbound phages. The gastrocnemius and heart muscles were isolated and washed three times with Tris-buffered saline (TBS). Subsequently the organs were homogenized in 2 mL TBS with MagNA Lyser green beads (Roche Diagnostics) in the MagNA Lyser (Roche Diagnostics). The suspension was titrated and amplified. Up to four rounds of biopanning were performed for each fraction. After the third and fourth round, phages were plated and colonies were picked. The selected peptides were synthesized with a FAM label and incubated with primary human control myotube cultures and analyzed with fluorescence microscopy. Clear fluorescence was observed for 7 of the 12 peptides. Based on the highest fluorescence intensity, P11 (HVIANAG) were chosen for in vivo evaluation. Mice were intramuscularly injected with 2.5 nmol of labeled peptide in the gastrocnemius muscle. Analyzed with fluorescence microscopy, P11 was found negative. 2358 DSSPYLMSPLGLDFD SPDDPPLPDLLYRSG FAPDLTRFPSVVVST SSADGGQGPHLLVRY EHFAPSSPDFLERHF NVSPDALEWLVGSKC DRRFFQSDILALFSP LPPPQQFHQDMMKLF RLPLDTFHSDLSRLT DTADLWWNSGTFLPA THASYMSPSSAFTLQ 11 Phage display (subtractive panning) 2 PMID:24361045 Mixtures of 13 anti-dystrophin monoclonal antibodies Dystrophin Not determined. Not determined. f88-15mer phage display library (X15) A sample of the phage library was pre-incubated in dishes coated with the rabbit anti-mouse antibodies alone to ensure any binding was specific for the target mAbs. Then unbound phages were incubated with monoclonal antibody mixtures. 2359 KQPAFNL(5) KAPAFDL(4) KLIAFDL(4) KLPAFML(2) KAPAFNL(1) KSPAFNL(1) 6 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of the peanut allergic patient no. 2205 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2205 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (intact Ara h 1). Three rounds of positive selection were performed. 2360 KAPAFDL(3) KSPAFDL(3) KLPAFML(2) KQPAFNL(2) KTAAFNL(2) KAPAFNL(1) KEPAFML(1) KLPAFQL(1) 8 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of the peanut allergic patient no. 2205 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2205 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (digested Ara h 1). Three rounds of positive selection were performed. 2361 ASPKSLL(1) FHTARPW(1) FSPIVLY(1) SPIMDYF(1) SPPRGIF(1) TSRADTL(1) TTSVRNT(1) VTLAPLR(1) S-P-I-x(2)-[HY]-Y 8 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of the peanut allergic patient no. 2208 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2208 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (intact Ara h 1). Three rounds of positive selection were performed. 2362 SPIISHY(2) SPILAHY(2) MSDRGIF(2) AKPASWA(1) DSPRGVF(1) SPIINYY(1) SPITLYY(1) VPPRGLF(1) YDVSSLP(1) S-P-I-x(2)-[HY]-Y 9 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of the peanut allergic patient no. 2208 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2208 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (digested Ara h 1). Three rounds of positive selection were performed. 2363 ATETSYR(1) HAPRGVF(1) LPPRGLF(1) MSDRGIF(1) SIPYPAP(1) SNATWVP(1) SPIINYY(1) STIMSSR(1) WSPIVHP(1) P-P-R-G-[LV]-F 9 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of the peanut allergic patient no. 2209 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2209 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (intact Ara h 1). Three rounds of positive selection were performed. 2364 QWAFAIP(2) DLTWAPK(1) FSPIIAF(1) GLAGYPP(1) LSPIIVY(1) MSDRGIF(1) NLVLFSY(1) SPILAHY(1) SPIVLYF(1) SPIVNNY(1) S-P-I-x(2)-[HY]-Y 10 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of the peanut allergic patient no. 2209 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2209 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (digested Ara h 1). Three rounds of positive selection were performed. 2365 IPYTLDK(3) TPYTLDK(2) AKQTDTM(1) DISRGLF(1) EWSRGIF(1) LHRPLHP(1) VTGPAKT(1) 7 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of the peanut allergic patient no. 2304 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2304 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (intact Ara h 1). Three rounds of positive selection were performed. 2366 LPYTLSK(3) AKQTDTM(2) ADMRGLF(1) AKGTDNW(1) GSAFSAF(1) GVYSLSK(1) IPYTLDK(1) SFPARFY(1) TPYTLDK(1) WPDRGIF(1) 10 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of the peanut allergic patient no. 2304 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2304 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (digested Ara h 1). Three rounds of positive selection were performed. 2367 FSPIVLY(2) SPITEFY(2) KSIYHQW(1) LPSRGLF(1) LTDLTQK(1) QSLTRLP(1) SPILAHY(1) TFKLHPI(1) TPPRVHL(1) VLPGSKS(1) WESRGVF(1) YPPKPLH(1) S-P-I-x(2)-[HY]-Y 12 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of the peanut allergic patient no. 2305 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2305 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (intact Ara h 1). Three rounds of positive selection were performed. 2368 VMPGSKP(3) KNVYHQT(2) ACCRSIP(1) AVESNDK(1) FPPKPKL(1) LINQALK(1) MSDRGIF(1) SPIISHY(1) SPITLYY(1) SPITTYY(1) TPFSNSP(1) S-P-I-x(2)-[HY]-Y 11 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of the peanut allergic patient no. 2305 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2305 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (digested Ara h 1). Three rounds of positive selection were performed. 2369 AQSQFNS(5) YSTQVRP(2) AYTLPSR(1) HFSKVPR(1) SNAARAY(1) YHPFLQV(1) 6 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of animal nr. 19 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with mouse-α-rat IgE. For positive selection, IgE of animal nr. 19, rat immunized with digested Ara h 1, were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (intact Ara h 1). Three rounds of positive selection were performed. 2370 AQSQFNS(2) APHRHPH(1) APKAAPL(1) AQLAPET(1) GGQFGPP(1) IHLPPAL(1) NQTRTTH(1) QPMINML(1) QPPTANA(1) TPMAWSQ(1) VHNTRLL(1) 11 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of animal nr. 19 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with mouse-α-rat IgE. For positive selection, IgE of animal nr. 19, rat immunized with digested Ara h 1, were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (digested Ara h 1). Three rounds of positive selection were performed. 2371 AYPLRAH(1) GLTPSKN(1) GPPPLPK(1) HHLHQTN(1) LPLPVVR(1) NLANKMA(1) SLYKPHP(1) SSYSQLN(1) VTAPFRV(1) YGTHKSP(1) YSIPKSS(1) 11 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of animal nr. 49 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with mouse-α-rat IgE. For positive selection, IgE of animal nr. 49, rat immunized with intact Ara h 1, were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (intact Ara h 1). Three rounds of positive selection were performed. 2372 TASGLYS(4) GPPPLPK(1) HHMHTTR(1) IAMKPLA(1) LPSLPRI(1) LQSKTLH(1) NTSNPYT(1) QATFSHS(1) STFLPHP(1) VPPQLSR(1) VTAPFRV(1) VYPMAMS(1) YHGSVSL(1) 13 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of animal nr. 49 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with mouse-α-rat IgE. For positive selection, IgE of animal nr. 49, rat immunized with intact Ara h 1, were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (digested Ara h 1). Three rounds of positive selection were performed. 2373 STFLPHP(7) AMPPLPP(1) HPWAPMQ(1) KPPNLPN(1) KWLPTPL(1) SASWQES(1) SLTSWAT(1) TQMSKHL(1) VIAKTRL(1) 9 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of animal nr. 52 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with mouse-α-rat IgE. For positive selection, IgE of animal nr. 52, rat immunized with intact Ara h 1, were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (intact Ara h 1). Three rounds of positive selection were performed. 2374 AQSQFNS(4) ALGASHG(1) FHDTPQS(1) HISLGRI(1) LGTYGHY(1) NPASSHM(1) NYLSLLH(1) QIPSGTP(1) QLSAPPP(1) SASLMLQ(1) TMSKDST(1) YPPMTQV(1) YTQGWNL(1) 13 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of animal nr. 53 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with mouse-α-rat IgE. For positive selection, IgE of animal nr. 53, rat immunized with intact Ara h 1, were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (intact Ara h 1). Three rounds of positive selection were performed. 2375 AQSQFNS(3) AKTSSNV(1) APLPKLM(1) DQWHRAP(1) FGFPTTS(1) FPTPRVA(1) HQPQQLF(1) ISVNIQA(1) IVQHTVP(1) KPWLPQH(1) NAHHTYP(1) NHTTLKA(1) NPLPSQL(1) QPVPPRL(1) SDRFPPK(1) SDYHRVM(1) SLDTCLR(1) SPSPQRS(1) TGGVWSK(1) YNPAVIA(1) 20 Phage display (subtractive panning, competitive panning) 3 PMID:22555070 IgE of animal nr. 53 Allergen Ara h 1, clone P41B Not determined. Not determined. Ph.D.-7 phage display library (X7) In negative selection, the library was incubated with mouse-α-rat IgE. For positive selection, IgE of animal nr. 53, rat immunized with intact Ara h 1, were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (digested Ara h 1). Three rounds of positive selection were performed.