2326 TLMVPRTGS(4) MASPRMLR(1) GAKPRALR(1) SRASRLKV(1) RWSPRSIYG(1) 5 Phage display (common panning) 6 PMID:11896050 Mast cell protease 4, mMCP-4 Not determined. Not determined. Not determined. X9 T7 phage display library 2327 YFRILRIKGLSS(2) SLSLITMLKISR(2) HMRTILPLKLTI(1) RPPIRNPININS(1) RPSYLSLIRILK(1) IHRYAEHRILPN(1) LRIIIKTRRLRL(1) RIQRIPILMINP(1) KPSRTIRILSHN(1) IPTIKSTKSIRN(1) RIYFIKWRRISL(1) QLRRRSMLITPH(1) RIRIHTPRLIIP(2) RMRTPIRHMILP(1) HHQRILPMRKIM(1) IKLLIKMNLKRN(1) LLKHRPRINKNL(1) RNKILTQRIINN(1) IPKIMHLLKRTM(1) RLHFILKWIIHR(1) RMRINQRNLMIN(1) AFPTRMRIQKTI(1) RPTLISLIYRMI(1) AYILPRIKSFTA(1) 24 Phage display (subtractive panning) 4 PMID:24014474 Hepatocellular carcinoma cell line HepG2 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2328 CGX(F/Y/W)(S/N)HPQC 1 Phage display (common panning) 5 PMID:15919886 Streptavidin Biotin Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2329 IPLPPPSRPFFK 1 Phage display (subtractive panning) 4 PMID:22791264 Platelet-derived growth factor receptor β (PDGFRβ) Platelet-derived growth factor subunit B Not determined. Not determined. Ph.D.-12 phage display library (X12) Both positive (PDGFRβ) and negative (FGFR) target were biotinylated. Each selection round was conducted as follows: e11 plaque-forming units were added in 500μl PBST supplemented with 0.1% BSA pH 7.0. After 30-min incubation, 50μl Dynabeads? M-280 Streptavidin (Invitrogen) was added together with 100 μl biotinylated negative target FGFR 100 nM and 100μl non-biotinylated FGFR 1,000 nM. After 1-h incubation at room temperature, the unbound phages were separated with a Dynal magnet and incubated with 100 μl biotinylated PDGFRβfor 1 h at room temperature. Eighty percent of the clones isolated on biotinylated target in suspension displayed the peptide sequence: IPLPPPSRPFFK. 2330 EHMALTYPFRPP(16) AYYPQNHKSKAE(15) APNHIPRPPGLT(10) YPHYSLPGSSTL(8) AHRHPISFLSTL(2) SILSTMSPHGAT(1) ITMSSNAEHSRI(1) 7 Phage display (subtractive panning) 3 PMID:19327883 NCI-H1299 non-small cell lung cancer cell line Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) NCI-H1299 cells were taken as the target cells, and the normal lung cell line (SAEC) as the absorber cells for a whole-cell subtractive screening from a phage display 12-peptide library. 2331 GRVRDQVAGW GRVKDQIAQL GVRDQVSWAL ESVREQVMKY SVRSQISASL GVRETVYRHM GVREVIVMHML GRVRDQIWAAL AGVRDQILIWL GRVRDQIMLSL G-R-V-R-D-Q-I-x(3)-L 10 Phage display (common panning) 3-4 PMID:9180079 Thrombopoietin receptor, TPO-R Thrombopoietin Not determined. Not determined. pⅧ and peptides-on-plasmids phage display library pool 2332 CTLRQWLQGC CTLQEFLEGC CTRTEWLHGC CTLREWLHGGFC CTLREWVFAGLC CTLRQWLILLGMC CTLAEFLASGVEQC CSLQEFLSHGGYVC CTLREFLDPTTAVC CTLKEWLVSHEVWC C-T-L-R-E-W-L-x(2-6)-C 10 Phage display (common panning) 3-4 PMID:9180079 Thrombopoietin receptor, TPO-R Thrombopoietin Not determined. Not determined. pⅧ and peptides-on-plasmids phage display library pool 2333 REGPTLRQWM EGPTLRQWLA ERGPFWAKAC REGPRCVMWM CGTEGPTLSTWLDC CEQDGPTLLEWLKC CELVGPSLMSWLTC CLTGPFVTQWLYEC CRAGPTLLEWLTLC CADGPTLREWISFC C-x(1-2)-E-G-P-T-L-R-E-W-L-x(1-2)-C 10 Phage display (common panning) 3-4 PMID:9180079 Thrombopoietin receptor, TPO-R Thrombopoietin Not determined. Not determined. pⅧ and peptides-on-plasmids phage display library pool 2334 CAAERGLFEDC(4) CTAWTYVLGPC(1) CTSWAYVLGPC(7) 3 Phage display (common panning) 3 PMID:11520027 Anti-E-3-G monoclonal antibody 41551 Estrone-3-glucuronide, E-3-G Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C) 2335 CERGPGKSRSCS CGNRVSKAPKS GRKVKCS RAAMEKPS RRAAVRMEKPCS 5 Phage display (subtractive panning) 1 PMID:11820288 Anti-MUC1 monoclonal antibody C595 Mucin-1, MUC-1 Not determined. Not determined. T7 CX9C phage display library The library was first screened against an 'irrelevant 'antibody that would not be expected to bind specifically to MUC1 epitopes. Anti-Estrone beta-D-glucuronide antibody was used for this purpose. 2336 CSRVAPNRK CVKRTASGSGCS CSMRASGGPKCS CTVPVRPQQKCS CPATTHLG CHLAGT CEE C 8 Phage display (subtractive panning) 2 PMID:11820288 Anti-MUC1 monoclonal antibody C595 Mucin-1, MUC-1 Not determined. Not determined. T7 CX9C phage display library The library was first screened against an 'irrelevant 'antibody that would not be expected to bind specifically to MUC1 epitopes. Anti-Estrone beta-D-glucuronide antibody was used for this purpose. 2337 RNREAPRGKICS(7) RNREAPRGKIC(1) RRPPMTTASCS(1) FERIAPKGGNCS(1) CSRGPAGRTVCS(1) CRAPAGSKKMCS(1) 6 Phage display (subtractive panning) 3 PMID:11820288 Anti-MUC1 monoclonal antibody C595 Mucin-1, MUC-1 Not determined. Not determined. T7 CX9C phage display library The library was first screened against an 'irrelevant 'antibody that would not be expected to bind specifically to MUC1 epitopes. Anti-Estrone beta-D-glucuronide antibody was used for this purpose. 2338 RNREAPRGKICS(4) NREAPRGKICS(1) CRPAPSAKVACS(1) FERIAPKGGNCS(1) CDSERTAPKCS(1) RQAGRKPVNNCS(1) CSRGPAGRTVCS(1) CSDRMPCEPSCS(1) RRPSR(1) 9 Phage display (subtractive panning) 4 PMID:11820288 Anti-MUC1 monoclonal antibody C595 Mucin-1, MUC-1 Not determined. Not determined. T7 CX9C phage display library The library was first screened against an 'irrelevant 'antibody that would not be expected to bind specifically to MUC1 epitopes. Anti-Estrone beta-D-glucuronide antibody was used for this purpose. 2339 WHWTWLSEYPPP LETSKLPPPAFL WHWRNPDFWYLK 3 Phage display (common panning) PMID:19813732 Gamma-gliadin and alpha/beta-gliadin Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2340 CATLDGVSC CFAGAGVRC CGGRHWVRC CGSVLPVLC CGSVSHRRC CIGGRWVVC CNSVRGSRC CRRHSVSGC CRSGRVSNC CRVWHRGGC CSAAGLARC CSGAFWGSC CSGWFAGSC CSPSSGTYC CVVWRGGIC 15 Phage display (subtractive panning) 2 PMID:16397212 Epidermal growth factor receptor Pro-epidermal growth factor, EGF 1IVO,1NQL,3NJP, Not determined. Ph.D.-C7C phage display library (CX7C) To isolate peptides binding to epidermal growth factor receptor (EGFR), phage clones selected on SKOV3 in rounds 2 and 3 of the screening were individually amplified and pooled, and e9 transduction units of the mixed phage were incubated overnight with 10 μg of purified human EGFR. 2341 LWCITQDCSHRM(4) HDAISWTHYHPW(1) 2 Phage display (common panning) 5 PMID:24111863 Aminopeptidase N Transmissible gastroenteritis virus, TGEV Not determined. Not determined. Ph.D.-12 phage display library (X12) MTT assay results indicated that the PM1 (HDAISWTHYHPW) phage clone had a protective effect against TGEV infection in ST cells, whereas the PM1 and PM4 (LWCITQDCSHRM) phage clones had no effect on swine testis (ST) cell growth. 2342 SVSVGMKPSPRP(4) LPWHFKSRHRYQ(1) EWMSHGHPRPNN(1) SLSTPATRHFSG(1) LSTPYSKSQAST(1) SHWNSHSTPARA(1) ALSTPTFSTLPA(1) 7 Phage display (common panning) 3 PMID:24130862 Anti-rTs-Pmy-N monoclonal antibody 8F12 Paramyosin, Ts-Pmy Not determined. Not determined. Ph.D.-12 phage display library (X12) Mice immunized with KLH-conjugated peptides 8A1 (ALSTPTFSTLPA), 8F1 (LPWHFKSRHRYQ) and 8F7 (LSTPYSKSQAST) and subsequently challenged with T. spiralis larvae resulted in a 22.7%, 22.2% and 26.2% reduction in muscle larvae burden, respectively. However mice immunized with th KLH-conjugated peptide 8F6 (SHWNSHSTPARA) had only 18.8% reduction in muscle larvae burden compared to the KLH control (p< 0.05). 2343 LSPPRYP(5) 1 Phage display (subtractive panning, competitive panning) 3 PMID:24142482 FGFR-expressing Balb/c 3T3 cells Fibroblast growth factors Not determined. Not determined. Ph.D.-7 phage display library (X7) The phage library was first incubated with FGFR-deficient Cos-7 cells. Then the supernatant was collected and added to the flask cultured with Balb/c 3T3 cells. For the last round of selection, low affinity cell-bound phages were first eluted with bFGF for 1 h, discarded, and high-affinity cell-bound phages were then eluted with bFGF for an additional 1 h. The synthetic P9 (LSPPRYP) peptide could inhibit the activation of two MAPKs (Erk1 and Erk2) in B16-Fl0 cells after stimulation by bFGF. And also the P9 peptide has a potent inhibitory effect on tumor growth in vivo, which was associated with significant inhibition of Erk1/Erk2 activation in tumor cells. 2344 FSDHWVN(6) 1 Phage display (common panning) 3 PMID:24145794 Anti-SAH monoclonal antibody ab111903 S-adenosyl-L-homocysteine, SAH Not determined. Not determined. Ph.D.-7 phage display library (X7) The original phage library was first incubated with anti-SAH Mab.The antibody-bound phages were then captured with Protein A or Protein G magnetic beads. Protein G magnetic beads were used in the first and third rounds. Protein A magnetic beads were used in the second round. To determine the affinity of the selected phage from the third-round, eight phage clones isolated and amplified. Six of these clones showed significant binding to the SAH antibody which was competitively inhibited by SAH, whereas two showed no significant binding. This strongly suggested that these six phage clones bound the antigen binding site of the SAH antibody. The six binding phage clones had the peptide sequence FSDHWVN. 2345 CRGATPMSC(1) CSEGLLNTC(1) CVQMPAHSC(1) CPNSTHRNC(1) CMHTHSRMC(1) CNTGSPYEC(1) CFSGMSTDC(1) CDASRPATC(1) 8 Phage display (common panning) 3 PMID:24145794 Anti-SAH monoclonal antibody ab111903 S-adenosyl-L-homocysteine, SAH Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The original phage library was first incubated with anti-SAH Mab.The antibody-bound phages were then captured with Protein A or Protein G magnetic beads. Protein G magnetic beads were used in the first and third rounds. Protein A magnetic beads were used in the second round. 2346 LRFLCWLDPDWMDCA(1) HGLSAWYLWALDAVG(1) ISGWYWWPIVDLYGA(1) QAMISPSRGFPRKYS(1) GSSFFYDVCSMWSLC(1) SLVYRSLFLDCHSDR(1) PFVDILVGLGLYPAG(1) DLTDFWNAFVPRIFG(1) VWLGFVDALHAFSGP(1) FLCMFAAHVAPYYCC(1) AYWFKCLALAYPGVA(1) FWGALLPPLLLTMGA(1) DPRASHLTGIRQLLQ(1) CFYDVASAIWASLSA(1) IFCVPSWLFSALYPD(1) DFWWSLLGDVLMVPQ(1) WFGLAADVGSKWLTW(1) VFPSPWAFLEPWWNT(1) ACFVDPSCWWSLLAR(1) FYLWSMLSALDAFTL(1) LLRYIPWLVPLPIGN(1) IFYSYLNAALGFPSI(1) VFDSLL 22 Phage display (common panning) 4 PMID:24251365 MA026 Not determined. Not determined. Not determined. X15 T7 phage display library Twenty seven single phage clones from the fourth-round elution were randomly picked out. Multiple sequence alignment analyses using CLUSTALW indicated that peptides 1 (FLPFWYNVFDSLSGN), 3 (FSAWLYSVFDQFSPA), 10 (GSSFFYDVCSMWSLC), 19 (CFYDVASAIWASLSA), and 24 (ACFVDPSCWWSLLAR) shared homology. Among them, the peptide sequence VFDSLL, a partially homologous sequence, was found. Then a single protein that includes the VFDSLL sequence, claudin-1 (CLDN1), was identified in the NCBI database by using BLAST. CLDN1 is highly expressed in the liver and plays an important role during the post cell binding process of HCV entry. 2347 CFDTRSLVC(3) CDHGYLPSC(2) CHYDGARAC(1) 3 Phage display (subtractive panning) 4 PMID:24265677 Mycobacterium Dleu/Dpan strain Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Three positive rounds of panning were performed against the targeted M. tb Dleu/Dpan strain. In order to remove peptides binding to cell wall components common to the mycobacterium genus, a subtraction round (round 4) was performed against M. smegmatis. A final positive panning round (round 5) was performed to enrich for peptides that are specific to M. tb. Ten plaques were selected using the traditional random cloning picking from the final round of biopanning, and were sequenced. Sequencing data of four of these plaques were ambiguous. Three unique sequences were obtained from the six remaining randomly selected plaques. 2348 CTYYWPLPC CSWYWPLPC CLMTSQFRC CWPIKVGWC 4 Phage display (common panning) 4 PMID:24265799 Ephrin type-A receptor 4 Type A/B ephrins Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) After four rounds of selection on EphA4, we detected an approximately 75-fold enrichment of phage binding to EphA4. In total, 48 of the individual clones that were tested for the binding to EphA4 Fc, and 6 of them showed significant binding when compared with the BSA negative controls. All 6 of these EphA4-binding clones displayed four different cyclic peptide sequences. The cyclic nonapeptide, TYY, CTYYWPLPC, binds to EphA4, but not to EphB4, and selectively inhibits the binding of ligand ephrinA5 to EphA4. The TYY cyclic peptide was evaluated for its cytotoxic effects on HUVECs, and it effectively inhibits HUVEC tubule formation activity. 2349 LSNNNLR 1 Phage display (common panning) 5 PMID:24284895 Silicon dioxide, SiO2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2350 GPIPGLLATVAV(2) LDTHASHACSTG(1) MATQPKLVGSPY(1) SNSPTFVCHRMV(1) SVVTSHQRYGTS(1) VQFPIEAMFWST(1) 6 Phage display (common panning) 2 PMID:24287902 Quinoprotein glucose dehydrogenase Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12)