2301 DHTLYTPYHTHP NHFGKFLDALAG 2 Phage display (common panning) 3 PMID:24073737 Bevacizumab (Avastin) Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The most frequently selected mimotope, DHTLYTPYHTHP (12P), was conjugated to KLH and used to immunize BALB/c mice. Furthermore, anti-12PKLH antibodies induced the inhibition of tube formation, proliferation and migration of HUVECs. Our data indicate that the isolated mimotope 12P could potentially elicit specific antibodies against VEGF and result in the inhibition of human umbilical vein endothelial cells. 2302 NMSIATVP(4) ASCYIDDGIRYCTG(4) VSCNPFSWLPRCYA(4) DYCNLSVSWCYP(3) STCWLMFSMCRV(2) TPCEGSWFFCTL(2) LGCDVIYWSSGWGCKF(1) GSCSLSIVTIPCGV(1) VTCAAFSLFPFCYN(1) CHPGWPCFW(1) GGCNLSVLWCLD(1) NECILVNGDRWCIS(1) FPCWALPSQYLCRP(1) TACNLSVSWCEF(1) TNCGFLYLGCVI(1) FDCGDGSWRYYLDCVR(1) TSCRVLWIMTLCNA(1) EVCNNKLWPCFW(1) GGCGWLWVSCVI(1) CSPAWPCWW(1) LPCAGRSFPCYW(1) DRCLLWVSLCMA(1) CIPMWPCWW(1) 23 Phage display (common panning) 4 PMID:23922242 U-CLL mAb068 Not determined. Not determined. Not determined. M13 pVIII phage display library 2303 NSPDYASRLAAR(6) SNYAALLTGHHH(3) EPYHLKLAQRSQ(1) DNYAAALAQRAR(1) EGNWATALSSRA(1) APNYALLLNSRM(1) NSPTYASLLWGR(1) HFPSTTLRQVTT(1) NY, A-[ALST], L, R, P 8 Phage display (subtractive panning) 3 PMID:19690339 M-CLL mAb169 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection on BSA-blocked Protein A and Protein G beads was carried out in rounds 2 and 3 before incubation with the CLL mAb. 2304 WSYNKDLLDTLE(7) FSYSHESLRTLI(1) FSYTEFTLRQLL(1) FTYSHSQLHMLL(1) NFGYSQRWLSLH(1) APQYSLQLLQRL(1) FAFDEKLLRNLM(1) AWTYHPLRWNWL(1) F-[ST]-Y-[ST], L-[HR], L-[LI] 8 Phage display (subtractive panning) 3 PMID:19690339 M-CLL mAb183 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection on BSA-blocked Protein A and Protein G beads was carried out in rounds 2 and 3 before incubation with the CLL mAb. 2305 SPNFSWLPLGTT(9) HWPRPDDSFWRP(2) NHTFRLASPPVL(1) ASPANNPSPAWL(1) FSMPFRPITPVH(1) KDWRHPNWSGVL(1) NTKPSLPTISWP(1) HPPGIFPWTSKA(1) ATWSHHLSSAGL(1) LDSRPWPRLYIT(1) HGYSMIAMHSIH(1) TFQIPSTFVWHR(1) FHGRHGLEPLQV(1) TPTSALLPPLAY(1) APQDNTIMPSPT(1) SQPFFAWLKNIP(1) [FW], P, W, T 16 Phage display (subtractive panning) 3 PMID:19690339 M-CLL mAb255 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection on BSA-blocked Protein A and Protein G beads was carried out in rounds 2 and 3 before incubation with the CLL mAb. 2306 YHHAYLLPPPRM(2) TDSYYHHPFSKY(1) LPNIPYHHPLYH(1) YHHPIVGQVQFI(1) LPPSAYFHHAYL(1) YSWAHHTYPPLT(1) HPNYHHGYPSVS(1) SPKSEHIHHSFA(1) ESWSQYYHHSHP(1) YHHMPMIGRTII(1) ALTYPSAYHHGW(1) DVVPTYNYAFHH(1) QDRLPNRWHTYI(1) P, YHH, [FY], [ST] 13 Phage display (subtractive panning) 3 PMID:19690339 M-CLL mAb412 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection on BSA-blocked Protein A and Protein G beads was carried out in rounds 2 and 3 before incubation with the CLL mAb. 2307 IPLPPPSRPFFK(27) SLVPHPRFTNHN(1) WGSSARYVLPMP(1) HYPTAKFHAERL(1) P 4 Phage display (subtractive panning) 3 PMID:19690339 U-CLL mAb014 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection on BSA-blocked Protein A and Protein G beads was carried out in rounds 2 and 3 before incubation with the CLL mAb. 2308 WPLWSIPPFSPR(10) TQTTVSSTQKPY(1) SGASRTPAALSY(1) SWASLPVGMNSW(1) QLHMNMSSRTDT(1) EAPQKLDPRYAQ(1) [ST], [VL]-P 6 Phage display (subtractive panning) 3 PMID:19690339 U-CLL mAb068 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection on BSA-blocked Protein A and Protein G beads was carried out in rounds 2 and 3 before incubation with the CLL mAb. 2309 WHDDQMLRRTVT(10) DTPYWWIPHFNA(2) SHRLDTLLLLAT(1) YHHSYPPIYTGW(1) H 4 Phage display (subtractive panning) 3 PMID:19690339 U-CLL mAb114 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection on BSA-blocked Protein A and Protein G beads was carried out in rounds 2 and 3 before incubation with the CLL mAb. 2310 FSLQTMRHWPYS(16) WTPDDGWRTNLT(1) SSNYQMYYQYFS(1) TTDKHSMTPAAS(1) QLIPHIDAVRLL(1) ASLQGMQTSSWA(1) MNPPWKHTMPRQ(1) QYNMVYAPDVPL(1) SPNFSWLPLGTT(1) TMASSMWPLNRW(1) MPAVMSSAQVPR(1) WPMRLEASYAKP(1) NTKPSLPTISWP(1) WVEILPTARPHG(1) TWIPSAWLIDWD(1) 15 Phage display (subtractive panning) 3 PMID:19690339 U-CLL mAb270 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection on BSA-blocked Protein A and Protein G beads was carried out in rounds 2 and 3 before incubation with the CLL mAb. 2311 WNLRDTCYPYCT(13) WHDDQMLRRTVT(5) TVKVTDFANLQR(1) MPANSPTSPAFP(1) ATPGITHYTSSA(1) FARGGGELPESM(1) NSMPVRPLLQDF(1) ETYLVRETLLRQ(1) TTVAKRKRTQSR(1) RVKMSKNKKNPA(1) AGVHFTATKAAP(1) LDFSYSRTSWAP(1) FHDNETLRKRVT(1) W, [ST]-[HKR], R 13 Phage display (subtractive panning) 3 PMID:19690339 U-CLL mAb114 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection was performed in rounds 2 and 3 by incubating the amplified eluate of the previous round with bead slurry (50 L) of Sepharose-polyclonal human IgG. 2312 CGFSGELGC 1 Phage display (subtractive panning) 2 PMID:24098722 Atherosclerotic plaques Not determined. Not determined. Not determined. CX7C fUSE5 phage display library The phage library in 100 μL of DME with 3% BSA was incubated with the luminal face of the normal carotid. Unbound phages were recovered and incubated for 2 hours with the luminal face of a fresh carotid plaque. Comparison to public databases using BLASTp indicated that one of the phages (phage B6, and only this phage) presented four consecutive amino acids (ELGC) in nine (CGFSGELGC) identical to plexin B1, rendering an alignment score of 16.3. 2313 WSGPGVWGASVK(3/31) TLSGAFELSRDK(3/31) TPWQDTIKPQRE(3/31) 3 Phage display (in vivo) 2 PMID:24105738 Ovarian cancer Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) SKOV3 suspensions were subcutaneously injected into the right flank of mice to generate epithelial ovarian cancer xenografts. The phage-displayed peptide library (New England Biolabs, Beverly, MA, USA), e11 TU was intravenously injected and circulated for 15 min. After anesthetized with intra-peritoneal injection of chloroquine, the mice were perfused through the heart. Then, the tumor tissues were collected, homogenated with a micro tissue grinder, and then washed, weighed and lysed with 1% NP40. Other 22 phage clones in the second round appeared once respectively, but their sequences were not shown. 2314 WSGPGVWGASVK(39/43) TLSGAFELSRDK(1/43) VTPTEHFTGSWR(1/43) HTHTTTHWFTSN(1/43) YPSELNVRTPHR(1/43) 5 Phage display (in vivo) 3 PMID:24105738 Ovarian cancer Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) SKOV3 suspensions were subcutaneously injected into the right flank of mice to generate epithelial ovarian cancer xenografts. The phage-displayed peptide library (New England Biolabs, Beverly, MA, USA), e11 TU was intravenously injected and circulated for 15 min. After anesthetized with intra-peritoneal injection of chloroquine, the mice were perfused through the heart. Then, the tumor tissues were collected, homogenated with a micro tissue grinder, and then washed, weighed and lysed with 1% NP40. To evaluate the homing efficacy, phage clone PC3-1 (WSGPGVWGASVK) and M13KE control phage were injected into the xenografts model. As a comparison, M13KE phage showed no immunoactivity. Based on these results, it was obvious that the peptide WSGPGVWGASVK displayed on PC3-1 was a suitable candidate for ovarian cancer-targeting peptide. 2315 HLYVSPW 1 Phage display (common panning) 4 PMID:23852533 Toll-like receptor 2 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) Five phage clones (E5, F7, Pep2, F11 and E6) were selected, but their sequences were not shown. The TLR2-binding CPP, HYLVSPW, is a promising candidate for targeted drug development. 2316 SNHWHSQGLVPR(6) ANLTPTGLVPRP(1) QLYDHLGLVPRL(3) GSPLTGLVPRWA(2) GLVPR 4 Phage display (common panning) 3 PMID:23420525 Anti-Efb-C polyclonal antibody Clumping factor A and B Not determined. Not determined. Ph.D.-12 phage display library (X12) 2317 STMWPSV(1/8) SVTNHPT(1/8) TDIQHAK(1/8) HLPVPLR(1/8) YPYPLIS(1/8) LHSELPT(1/8) SSLSLTP(1/8) RPMNIGS(1/8) 8 Phage display (subtractive panning) 3 PMID:23784990 Ceramic fluorapatite Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) The original phage library was diluted in 1 ml TBST (0.1%) buffer and incubated with the blocked empty column (without CFA sample) at room temperature. After 1 h of incubation, the phage solution was discarded. The discarded solution contains the phages that did not show strong affinity to the column materials and was used for the selection of CFA-binding phages. 2318 HLPPWTQ(1/10) KPRSMLH(1/10) KLHLPLH(1/10) MVHAQRP(1/10) AYHPDAT(1/10) SQAHFYE(1/10) SKTKNNL(1/10) KTTGLTV(1/10) 8 Phage display (subtractive panning) 4 PMID:23784990 Ceramic fluorapatite Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) The original phage library was diluted in 1 ml TBST (0.1%) buffer and incubated with the blocked empty column (without CFA sample) at room temperature. After 1 h of incubation, the phage solution was discarded. The discarded solution contains the phages that did not show strong affinity to the column materials and was used for the selection of CFA-binding phages. 2319 SQMHLRY(1/25) KGFHGYT(1/25) LLTKNSL(1/25) KPRSVSG(11/25) VMAPHKY(1/25) QRHTRPN(1/25) YLPPYKS(1/25) DHPAVAQ(1/25) SSPDKSF(1/25) HRPYIAP(1/25) ASEQVHM(1/25) SRLTMHT(1/25) HEVSDYL(1/25) THSKPLH(1/25) NKITQHS(1/25) 15 Phage display (subtractive panning) 5 PMID:23784990 Ceramic fluorapatite Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) The original phage library was diluted in 1 ml TBST (0.1%) buffer and incubated with the blocked empty column (without CFA sample) at room temperature. After 1 h of incubation, the phage solution was discarded. The discarded solution contains the phages that did not show strong affinity to the column materials and was used for the selection of CFA-binding phages. The peptide F5-4 ((KPRSVSG)), displayed by more than 40% of the phages analyzed in the fifth round of selection, was subjected to further studies. Selectivity of the peptide for the chemical composition and morphology of CFA was assured by the adsorption studies. The dissociation constant, obtained from the F5-4/CFA adsorption isotherm, was in the micromolar range, and the maximum capacity was 39.4 nmol/mg. 2320 MQNPTQAPSVGL(20) QDPTNSGFYGRP(3) LPLRTDPWAKAS(2) 3 Phage display (common panning) 2 PMID:23740479 Palladium powder Not determined. Not determined. Not determined. Ph.D.-12 phage display and wild type library pool 2321 AGRSYW(16) QARVAE(15) RAVLEA(1) RLQSRT(2) KPTTGR(2) QLQSRL(1) RLQSRR(1) 7 Phage display (common panning) 5 PMID:24219291 Serine protease 29 Not determined. Not determined. Not determined. T7 X6 phage display library 2322 CMSYEGSWRKWVMWGGC CDLFVMAVGTNLDWWGC CVERCLASTSSGVKALC 3 Phage display (common panning) 5 PMID:23896727 Tumor necrosis factor receptor superfamily member 5 CD40 ligand, CD40-L 3QD6, Not determined. pComb8 CX15C phage display library Among 5 selected phage colonies, the most frequently observed peptide sequence was NP31 (CMSYEGSWRKWVMWGGC) (60%), while 20% of clones were identical to the NP26 (CDLFVMAVGTNLDWWGC) and NP58 (CDLFVMAVGTNLDWWGC) clone sequences. 2323 DGNVVTCWACREKRW 1 Phage display (common panning) 5 PMID:23896727 Tumor necrosis factor receptor superfamily member 5 CD40 ligand, CD40-L 3QD6, Not determined. pIF15 phage display library 2324 WHKQSQWPFGVW(10) AQIPSSLFYPPR(1) WAYPERAVFQFL(1) NTLNWKWFSPPF(1) SLAYPTPDARHL(1) SFTPPFPRLSLT(1) LLADTTHHRPWT(2) WHYNSQLIETLS(2) HQLPISISPHYP(1) WHYNSQADIDSF(1) GHKQSQWPFGVM(1) ETQSLPVQSMPP(1) WHQNWMYTAMQL(1) HHKTVWFSNFHS(5) TNPTLPA(1) EHPTSHINSTRP(1) TMGFTAPRFPHY(1) TLSNLSRVHAVL(1) HTMMVLPLGAKS(1) SLTVPFLPLYVP(1) TYTTAGSASRLV(1) HTRHITQAATTA(1) SQNVRSDPWKSQ(1) SADMHKRSGGPY(1) QFLDLHMRVTPV(1) NLTHPHRIPWFE(1) TMGFRAPRFPHY(1) SDNVHTWQAMFK(1) SAHGTSTGVPWP(1) KYPIQTSWLDSV(1) YQPMYSLLLRPG(1) TYTTAGQSSRLV(1) GNSWFIEQVVLL(1) TMGFTRPRFPHY(19) SQPFFAWLKNIP(1) NHLPGSPPETRS(1) HITLRMTDTESR(1) SVSVGMKPSPRP(1) 38 Phage display (common panning) 3 PMID:22171001 Replication initiator protein Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2325 AVEYYRFDS(1) DEVVFYLGF(1) DMVWLMYRL(1) ELVLFMGGW(1) EWMVWFTGV(1) GFSFFWGRW(1) GLMFFQGGW(1) GLTWFMGTW(1) IEWYSGRLW(1) IIWYVALPE(1) LLYFRGVEV(1) LMWFRGAFD(1) LMWFRGLSI(1) LVLFASRVV(1) LVMFSGTYW(1) LVWYNLPLS(2) LVYYVSRRM(1) MLTWFFSTP(1) RWMLVWFRR(1) SLVWFRSKF(1) VAGLGSFGH(1) VVWFRGVFS(1) VVWYSLVPY(1) VVWYVATLS(1) WIWYNSVLK(1) WLFFNGSLW(1) WLVWFRGQR(1) WVFFQSALP(1) WVFFSYSEL(1) YIWFLGEMP(1) YTYYSLLVV(1) LVWFRG 31 Phage display (common panning) 6 PMID:11896050 Mast cell protease 4, mMCP-4 Not determined. Not determined. Not determined. X9 T7 phage display library