2276 ATSLPPTEHPNMYQG(1) AGGYSPTEHAFHSPP(1) TSTPTEHTYPLEIIT(1) DPGTEHAGVPLRHSA(1) YGARPPEHLLYSRAS(1) SPLPEPPPEHRALVP(1) YMQPDPPPPLHAPDY(1) TRMPLPNHYEPPPRT(1) PPPEH 8 Phage display (common panning) 3 PMID:10423148 Anti-gG2 monoclonal antibody E5 Envelope glycoprotein G, gG, gG-2 Not determined. Not determined. f88-15mer phage display library (X15) After three rounds of biopanning, individual phage clones were isolated and screened by ELISA to identify those which bound strongly to the antibody of interest, and those which gave a clear positive signal were sequenced. 2277 ALSSQGGMSPEPTPL(2) VSSRPTTHYYLPEPL(1) TPESTPLLPPFPRSV(1) STNPEPLPFPAEELS(1) QKYAPETTPVSYLGA(1) HVLSSRPTTLALPLF(1) DYTPQTSLELPPESF(1) TPAQAYPALRSLIPW(1) TATTVTPRRTPYAPI(1) TPL 9 Phage display (common panning) 3 PMID:10423148 Anti-gG2 monoclonal antibody F11 Envelope glycoprotein G, gG, gG-2 Not determined. Not determined. f88-15mer phage display library (X15) After three rounds of biopanning, individual phage clones were isolated and screened by ELISA to identify those which bound strongly to the antibody of interest, and those which gave a clear positive signal were sequenced. 2278 QPTSKPTRPWLVSFL(1) NAETAFSLSSFPPSP(1) 2 Phage display (common panning) 3 PMID:10423148 Anti-gG2 monoclonal antibody H7 Envelope glycoprotein G, gG, gG-2 Not determined. Not determined. f88-15mer phage display library (X15) After three rounds of biopanning, individual phage clones were isolated and screened by ELISA to identify those which bound strongly to the antibody of interest, and those which gave a clear positive signal were sequenced. 2279 RGRGAG LGRRRS IRGRWA AGRSAF GRTDFW IKRNIT PRGRGG NKYARQ YHIKRS [GA]-R-x-[AG] 9 Phage display (subtractive panning) 1 PMID:7644467 Tissue-type plasminogen activator Plasminogen, EC=3.4.21.7 Not determined. Not determined. f3-6mer phage display library (X6) For positive selection, the phage library was digested overnight at 37°C by concentrations of t-PA varying from 10 to 100 μg/ml. The reaction mixture was then placed on ice, bovine serum albumin was added to 0.1%, and 100 μg of mAb 179 and 10 μg of mAb 3-E7 were added. After 30 min on ice, 100 μl of Pansorbin cells was added, and the reaction mixture was rotated at 4°C for 1 hr. The mixture was microcentrifuged for 2 min, and the supernatant was recovered to repeat the Pansorbin adsorption. 2280 VLRSAR QYLRYG KDARRA WLPRRA ILRAAY LRGRTA [GA]-R-x-[AG] 6 Phage display (subtractive panning) 2 PMID:7644467 Tissue-type plasminogen activator Plasminogen, EC=3.4.21.7 Not determined. Not determined. X6 fAFF1-tether C phage display library For positive selection, the phage library was digested overnight at 37°C by concentrations of t-PA varying from 10 to 100 μg/ml. The reaction mixture was then placed on ice, bovine serum albumin was added to 0.1%, and 100 μg of mAb 179 and 10 μg of mAb 3-E7 were added. After 30 min on ice, 100 μI of Pansorbin cells was added, and the reaction mixture was rotated at 4°C for 1 hr. The mixture was microcentrifuged for 2 min, and the supernatant was recovered to repeat the Pansorbin adsorption. 2281 GFARRA FGRRRT TRARRA [GA]-R-x-[AG] 3 Phage display (subtractive panning) 3 PMID:7644467 Tissue-type plasminogen activator Plasminogen, EC=3.4.21.7 Not determined. Not determined. X6 fAFF1-tether C phage display library For positive selection, the phage library was digested overnight at 37°C by concentrations of t-PA varying from 10 to 100 μg/ml. The reaction mixture was then placed on ice, bovine serum albumin was added to 0.1%, and 100 μg of mAb 179 and 10 μg of mAb 3-E7 were added. After 30 min on ice, 100 μI of Pansorbin cells was added, and the reaction mixture was rotated at 4°C for 1 hr. The mixture was microcentrifuged for 2 min, and the supernatant was recovered to repeat the Pansorbin adsorption. 2282 QRGRKA IVRRAE VARRAA [GA]-R-x-[AG] 3 Phage display (subtractive panning) 4 PMID:7644467 Tissue-type plasminogen activator Plasminogen, EC=3.4.21.7 Not determined. Not determined. X6 fAFF1-tether C phage display library For positive selection, the phage library was digested overnight at 37°C by concentrations of t-PA varying from 10 to 100 μg/ml. The reaction mixture was then placed on ice, bovine serum albumin was added to 0.1%, and 100 μg of mAb 179 and 10 μg of mAb 3-E7 were added. After 30 min on ice, 100 μI of Pansorbin cells was added, and the reaction mixture was rotated at 4°C for 1 hr. The mixture was microcentrifuged for 2 min, and the supernatant was recovered to repeat the Pansorbin adsorption. 2283 FTGRDI GFARAA IGRRAQ KAIGRM LGRKAT LKGRRA MRGRRG MRLRRA PFGRSA PYSRMA QLGRKA RGRRAR RGRSAG RRGRRA RYARRL RYARSA SRARKA SYLRRA TFARRA TVMRRA VARRAA WLGRRG YIGRRG [GA]-R-x-[AG] 23 Phage display (subtractive panning) 5 PMID:7644467 Tissue-type plasminogen activator Plasminogen, EC=3.4.21.7 Not determined. Not determined. X6 fAFF1-tether C phage display library For positive selection, the phage library was digested overnight at 37°C by concentrations of t-PA varying from 10 to 100 μg/ml. The reaction mixture was then placed on ice, bovine serum albumin was added to 0.1%, and 100 μg of mAb 179 and 10 μg of mAb 3-E7 were added. After 30 min on ice, 100 μI of Pansorbin cells was added, and the reaction mixture was rotated at 4°C for 1 hr. The mixture was microcentrifuged for 2 min, and the supernatant was recovered to repeat the Pansorbin adsorption. 2284 MGTTHLPYQFSL(3) 1 Phage display (common panning) 3 PMID:24040227 Listeria monocytogenes serotype 4b Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2285 KQATFDDYPVAH(7) MGTTHLPYQFSL(2) 2 Phage display (subtractive panning) 4 PMID:24040227 Listeria monocytogenes serotype 4b Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The first three surface biopanning rounds were performed against L. monocytogenes to select for phages binding to L. monocytogenes. The fourth included an initial subtraction biopanning step performed against L. innocua and using the supernatant derived from that for the subsequent biopanning against L. monocytogenes. 2286 KQATFDDYPVAH(3) KLHISKDHIYPT(2) 2 Phage display (subtractive panning) 4 PMID:24040227 Listeria monocytogenes serotype 4b Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) One solution biopanning was performed following the completion of the third surface biopanning round. The first three surface biopanning rounds were performed against L. monocytogenes to select for phages binding to L. monocytogenes. The solution biopanning included one solution biopanning against viable L. innocua and one against viable L. monocytogenes 4b. 2287 GPLATLHLPHKT(2) 1 Phage display (subtractive panning) 5 PMID:24040227 Listeria monocytogenes serotype 4b Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Two solution biopannings were performed following the completion of the third surface biopanning round. The first three surface biopanning rounds were performed against L. monocytogenes to select for phages binding to L. monocytogenes. Each solution biopanning round included one solution biopanning against viable L. innocua and one against viable L. monocytogenes 4b. 2288 ANAGPIMTTSLL(1) G*V*KPPQNVNR(1) GAKHPAQPHMMY(1) GAMHLPWHMGTL(3) GAMHLSVAYGYA(1) GEVDFNPR*DCA(1) GIIYDNPRKELN(1) GIIYSRGPEKLL(1) GIIYTLPAARYD(1) GIVFTLPALAHN(2) GKLFSSLDGL*F(1) GKLFSSPMDYDS(4) GKLYSHPLNNAK(5) GLLWTHPQTHGR(18) GMIWNEPKTWPG(1) GMIYVKPARPML(1) GNLFASPQKMHR(12) GPIFSAPT*TTI(1) GPIFSNSWGLIT(2) GPIFVNSDKGER(1) GPIHVAAFKNMT(1) GPILDMGFFNRE(1) GPIMSLPHRTVG(1) GPIMSLPTPTNL(1) GPINSKPSHMHI(1) GPIRDIGPVMDH(3) GPIVDSGGTHPR(1) GPIVSMPMPRLL(1) GPIWDNMPSRQV(1) GPIWSGRLIAQD(1) GPIYETIKTRTP(1) GPIYQQQNTILR(1) GPIYSTQHMKTS(3) GPLFDQGTQAYA(2) GPLHSSPLKISS(2) GPLISTPRHMNI(4) GPLVDLGPGDLR(6) GPLWTGQSQGSP(1) GPLYESRMPQNH(2) GPLYISSLTQLA(1) GPLYIVSHDTPR(1) GPVHSHPNDYSR(2) GQVYDVPYSRPK(2) GRIADLPPLKPN(3) GRIATLPDPTPR(2) GTDLD*AAAS*A(1) GTIFDYGPHGYA(1) GTIFDYGPPDMP(13) GTIWSQPGAISL(1) GVIWSDPKTASS(1) GVIYDKPA*KLH(11) GVIYDSHGPGRY(1) GVIYSKPNSVQL(7) GVIYSSDRDWRS(1) GVIYTDSLTRPH(1) GVMCKHPQTHGH(1) KLHISKDHIYPT(6) KQATFDDYPVAH(5) LYAKKPLLNPNR(2) NRPDSAQFWLHH(4) QSWPAAA*AFTS(1) TSMDSVSVIDLG(1) TSSQGDRLYVYK(2) TSWPSLSTSARS(1) VNLEHGYYHAPS(1) 65 Phage display (subtractive panning) 5 PMID:24040227 Listeria monocytogenes serotype 4b Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Two solution biopannings were performed following the completion of the third surface biopanning round. The first three surface biopanning rounds were performed against L. monocytogenes to select for phages binding to L. monocytogenes. Each solution biopanning round included one solution biopanning against viable L. innocua and one against viable L. monocytogenes 4b. 2289 ANATFHGYPTRS(1) FFPREYYSIEAP(1) GGNGPKGNNVVH(1) GKIWTEPPPPKP(1) GKIYNDPRMMSN(1) GKLFSSPMDYDS(1) GKLYSHPLNNAK(2) GKYHEKHNENMH(1) GLIWDLSWCSSK(1) GLLWTHPQTHGR(1) GMIWNEPKTWPG(1) GMIWSRPSAEKF(1) GNLFASPQKMYR(2) GPIATLPKGGGQ(1) GPIFSNSWGLIT(5) GPIHEAPSSPSG(2) GPIIA*TYPKRE(1) GPILDMGFFNRE(1) GPIMSLPHRTVG(1) GPIWSSIQTLPT(1) GPIYSSVKDGQR(1) GPIYSTQHMKTS(1) GPIYTDKSELGN(1) GPLATLHLPHKT(1) GPLFDQGTQAYA(1) GPLFITSAPPTK(1) GPLFSDPEPAKN(1) GPLISTPRHMNI(1) GPLVDLGPGDLR(1) GPLWTGQSQGSP(3) GPLYESRMPQNH(1) GPLYESSQVIRA(1) GPLYSSMASALA(1) GPLYSTNLPTRN(2) GPLYSYPFSMIE(1) GPSHNTLSPLLT(1) GPVHSHPNDYSR(1) GPVLDPLTPSTI(1) GQIYTTRDSLLG(1) GQKPT*NLDLKL(1) GQVYDVPYSRPK(2) GSIYAHPRWLKW(1) GTIMTLANTERP(1) GTIQVHPPAPAR(1) GVIWSDPKTASS(1) GVIYSKPNSVQL(1) GVIYSSDRDWRS(1) GVIYSTHDTRPY(1) GVLHSSPNHRWQ(1) GWHKHKSMSAPL(1) HLINTNAQIAQR(1) IQLEMGGTRFHR(2) KDDASPAWNSRH(1) KHMMINAYRMTE(1) KIHKTESTPAWF(1) KKGDV**L*LRR(1) KLHISKDHIYPT(4) KNLHVGSYPQPI(1) KPHAHKHNDYFL(1) KPHHPHKIPYTN(1) KPHNMTELHHKH(1) KPHYDHRLHQPI(1) KQATFDDYPVAH(24) KQSDVR*VSWWA(1) KRRAKQKTGEQR(1) LDLQTPGHKWSQ(1) LLPPT*ATVGAR(1) LSCTTSVACLQT(1) NVATKSSGHNMR(1) RQVRMHPLDSWS(1) RRKMKQTEKMKI(1) SKSRAKGKQAKN(1) SLNRKKRRTHAK(1) SLRQVNTHTWLT(1) TDAKMRAKFPGH(1) TQARCNEYPVGH(1) TSMDSVSVIDLG(1) VNLEHGYYHAPS(3) VNLQTGWYTMAS(7) VNSADWVVCDGV(1) VSLPMGFYSMNS(1) YLPISQTHNRNV(1) 82 Phage display (subtractive panning) 4 PMID:24040227 Listeria monocytogenes serotype 4b Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) One solution biopanning was performed following the completion of the third surface biopanning round. The first three surface biopanning rounds were performed against L. monocytogenes to select for phages binding to L. monocytogenes. The solution biopanning included one solution biopanning against viable L. innocua and one against viable L. monocytogenes 4b. 2290 CIQTTWSRC 1 Phage display (common panning) 5 PMID:15987688 Amyloid β peptide, Aβ40 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Thirty Aβ40 -binding clones were individually picked, amplified, and had their DNA sequenced. Most of the 30 clones thus obtained had different DNA sequences. The IQ peptide (CIQTTWSRC) may be a lead for the development of novel drugs to block the inhibition of nAChRs in AD. 2291 RRWVRYPVHLHSPIV(1) PPYHRFWRGHRHAVQ(12) HRISHFAHRYLARLH(2) WHWRHRIPLQLAAGR(2) GWHSLLHSRYHRIAA(1) FVWVRFHRLPRQIYT(1) WHKYFLRRPLSVRTR(4) VPMALNHGVYVMVSS(1) RKWFLQHRRMPVSVL(6) SGRRHLHRHHIFSLP(2) GWITFHRRHHDRVLS(1) RLHGHRSHRFTHVAQ(1) KRIWFIPRSSWYERA(3) MPLSRYWWLFSHRPR(1) RHLSHFKWLRSHGLD(1) RRFHFHSRMVAVDNS(1) 16 Phage display (common panning) 3 PMID:17804251 Transforming growth factor beta-1, TGF-beta-1 Transforming growth factor beta receptor type 3, TGF-beta receptor type 3, TGFR-3 Not determined. Not determined. f5-15mer phage display library (X15) Each selection was performed on the streptavidin-coated plates. In vitro and in vivo experiments were performde to valuate their capacity to neutralize TGF???21. A murine model of acute liver damage was used to test the in vivo activity of all 20 phage-derived peptides. As found in vitro, P17 (KRIWFIPRSSWYERA) was also the most active in vivo followed by P6 (PPYHRFWRGHRHAVQ), P4 (RLAHSHRHRSHVALT), P14 (SGRRHLHRHHIFSLP) and P11 (WHKYFLRRPLSVRTR). Other peptides such as P1 (DRRIFWWSLRSAPGA), P3 (RFFTRFPWHYHASRL), P7 (HRISHFAHRYLARLH), P9 (GWHSLLHSRYHRIAA), P15 (GWITFHRRHHDRVLS), P19 (RHLSHFKWLRSHGLD) and P13 (RKWFLQHRRMPVSVL) were without effect on the expression of collagen type I mRNA whereas peptides, P2 (HVRLHHYLRHRSLPN), P5 (RRWVRYPVHLHSPIV), P8 (WHWRHRIPLQLAAGR), P10 (FVWVRFHRLPRQIYT), P12 (VPMALNHGVYVMVSS), P16 (RLHGHRSHRFTHVAQ) and P18 (MPLSRYWWLFSHRPR) enhanced expression of collagen type I mRNA. 2292 CRMSRRSNC CMRNRPKRC CRRRLNRTC CRMRIRRNC CHPHPRPRC 5 Phage display (common panning) 3 PMID:20428769 Anti- FASN monoclonal antibody K1 Fatty acid synthase, EC=2.3.1.85 Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) A mimotope CMRNRPKRC against anti-FASN autoantibody can be used as an efficient diagnostic method of HCC. 2293 GNLFGRD(1) LSFPDWP(2) HSWLPYL(1) YSWTPH(1) QVLMMRP(2) YLIPPGL(1) FTPPVTL(1) TYLPGPL(1) TVSPGMK(1) DPPREIS(1) WHQTVPF(1) SISFWTL(1) IAKPGFW(1) WPKFHPS(1) GSSLDDS(1) SHLAPAL(1) TQSLSPP(1) IPLPQSP(1) 18 Phage display (common panning) 3 PMID:23845304 Anti-PCV2 Cap polyclonal antibody Capsid protein Not determined. Not determined. Ph.D.-7 phage display library (X7) 2294 VTAMEPGQ(28) AFNPEPGQ(11) VTALEPGQ(7) DTPPGWDQ(5) 4 Phage display (subtractive panning) 3 PMID:23885226 Rat mesenchymal stem cells, rMSCs Not determined. Not determined. Not determined. fd-tet X8 phage display library The landscape phage library was first depleted with culture flask to remove the phages that specifically bound to the culture flask. The depleted phage library was then incubated with target rMSCs. 2295 YEQDPWGVKWWY(8) HLSWLPDVVYAW(2) 2 Phage display (subtractive panning) 3 PMID:24046373 M2 macrophages Not determined. Not determined. Not determined. Ph.D.-12 and Ph.D.-C7C phage display library pool For the subtractive pans, 2.0e11 pfu of the amplified eluate was first applied to receptor-cleared M1 cells. M1 cells were centrifuged, and the supernatant was applied to M2 cells. Phage was eluted from M2 cells and amplified. Subtractive panning was repeated three times, using the amplified eluate from the previous pan as the starting library for each round. M2pep (YEQDPWGVKWWY) shows selective binding and internalization in M2 macrophages but with minimal M1 macrophage interaction. We demonstrate this peptide's ability to identify murine M2-like TAMs within mixed cell populations isolated from mouse colon carcinoma tumors and to accumulate in TAMs after i.v. injection into tumor-bearing mice. We show that i.v. administration of M2pep fusion peptides with KLAKLAKKLAKLAK (KLA), a proapoptotic peptide, to tumor-bearing mice selectively reduces TAM populations and prolongs survival. 2296 NQYMSNGLVWAL(1) GDWPAGQMARSA(1) SPWSPPFWNDDM(1) QGPQTDSAPPRF(1) GFNTNPPSFPRP(1) GPLKAYILPPKA(7) AAHQPPAQSDFL(1) WSAPGLSSSSAP(1) LSSSAVTNNTSS(1) DSPTVAHNTSPT(1) HETVQHNKGWMI(1) IEHNGKAWRIPQ(1) EVAAFSMPGRFS(1) YVEPQEQSMPYL(1) NIQLELNPRHLI(1) SNSTREFNPNMF(1) HHQNTYANYPRH(2) WPHHHSRHNHNH(1) STHGWMNDRHHP(1) KSITSNDGFNTL(1) GSITTQTAIYFP(1) LTSAISPQHGEY(1) SHSFHTQERTTH(1) AVFSQLPRTPHL(1) SLPEAPIRQYQG(1) HVTMSWPQTAQN(1) SNAGGLMSRTWE(1) KIMPDSWAIKPW(1) NVMIDKHNVNGS(1) DSYMHAYSWRTK(1) SFQNSTLHGPVY(1) HIGHDNHLYPNR(1) NARVLHTGNESL(1) DRAMTPIYNPYI(1) TYTDNGYFKRST(1) TATIKSEAKSPS(1) YVEQVSTGKARS(1) RTRMRSINSPNL(1) SYYGKTDTADLT(1) VVDIRSQFANQQ(1) 40 Phage display (common panning) 4 PMID:24039747 Bacteriorhodopsin, BR Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2297 KVWSVPQLSHQL(1) KVWLLETSHISL(1) KLWYLNPPPGSF(2) K-[VL]-W 3 Phage display (common panning) 4 PMID:24039747 Nanodiscs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2298 MQLPKADARHPH YQITLPYRYEMP TFSWEFTGWWGQ LTFPVTTTPPAV MTHNMHGPNSEP HALTPIKYIPPG SSAYYYHYEYFH LSISPQHALVFA AMYHGHYTITRW 9 Phage display (common panning) 3-6 PMID:23830854 Membrane protein, M protein Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) To determine the impact of pepTGEV-M7 (HALTPIKYIPPG) on TGEV infection, three separate approaches were used: (1) TGEV was treated with peptide before inoculation of ST cells; (2) ST cells were treated with peptide prior to TGEV inoculation, and; (3) ST cells were infected with TGEV before being treated with peptide. These results indicate that pepTGEV-M7 is able to interfere with the ability of the virus to infect ST cells. The antiviral effects of pepTGEV-M7-treated virus were further investigated by IFA. Results demonstrated a dosedependent reduction in the infection of ST cells in the presence of pepTGEV-M7. 2299 NRPDSAQFWLHH YSAQFPLYTSWS WSEYDIPTPQIP GNLFASPQKMYR LNQSPQLQNKYR STFAGHVMTSVQ FPIKDPSNTFQY DFPISYRSWQIS RYWDTQLSPQML YQLQLPVWNDWA NMSHEMQLYALA WQLQLPVWSSSD ENMIVRLQSFQR EFFQYWEHIMWF NEPDFYAWQHFM WMWPSHMQEFSW VPFNWHWMQHYD YTVMDLQDYYSP GQLYMWMGLPIN QTTQAQTMLHHV SPPSTRVQNTAL QDWQQGSRVLWL TDYNLWTRAQFI WSSSAMQTIQTA MPAWEGPAQIQS MQRDNGPHKTLR FQCLRIPCVYAW ETWYSEQPWSML MLAHPSIYSSQP QFQWWYYSDQSE THWAKVSTDQHQ RIGTRRESKQNE QLPVRIQGNYLA LQQTYLKNPRSG MHDEVSVHQSHV NAHQKHAGAGAR QMAMRAHTEGKN YTSNTQSFTSSL WSFELPEQLFRG WDLTQRQRDLYF ANMEHTKHVTQA DKPGYMLLDSQP WRCRIELLMHQD STPSLLFYQVES QSTWWEIAGYTP MPNRPWIGILAG 46 Phage display (common panning) 5 PMID:23876241 Protein-glutamine gamma-glutamyltransferase Z Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2300 LGCDDTAVAHWPWVY SDAYDTAVAHWPGFI MPPNLIANYESALSR YESPGFLLSGYDDTG WAAHPSGPSIVPRSL 5 Phage display (common panning) 2 PMID:23939045 Anti-SMN monoclonal antibody MANSMA1-21 Survival motor neuron protein Not determined. Not determined. f88-15mer phage display library (X15) Three of these 22 mAbs were responsible for the selection process. These mAbs included two of the mostly widely-used for SMA studies, MANSMA1 and MANSMA12, and one mAb against the proline-rich region encoded by exon 5, MANSMA3. Biopanning with the remaining 19 anti-SMN mAbs alone failed to select any further phage clones.