2226 SKQHPHH(1) SNSLISG(1) DQRLPFL(1) MHPHQTN(1) ALRPPPH(1) QSGSPTF(1) EPLQLKM(1) NLTLDPC(1) EPLQLKM(3) 9 Phage display (common panning) 1/2/3 PMID:23315991 Appetite-regulating hormone Growth hormone secretagogue receptor type 1 Not determined. Not determined. Ph.D.-7 phage display library (X7) A search in the mimotope database MimoDB 2.0 revealed that the peptide EPLQLKM has already been selected by other research groups using various targets. The same peptide was also found in different articles and patents using Google search engine. It was considered a false positive result. 2227 GPRPPSAPNMPL(5/10) 1 Phage display (common panning) 3 PMID:23643267 Serum from AS patients Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2228 CGQEISGLC[12.780 ± 0.633] CRIRMSAGC[6.543 ± 1.054] CWGGLSGLC[4.433 ± 0.727] CRVIVGPRC[NT] CRVFAGKRC[NT] CRVTRGHGC[NT] CIRVEAGSC[NT] CLRSGGLTC[NT] CEFGLSEVC[NT] CMIAGLC[NT] G(2)-L-S-G-L, ISGL, MSAG, RVTXG 10 Phage display (subtractive panning) 3 PMID:12629410 Human urothelial cells Not determined. Not determined. Not determined. fUSE5-based CX7C phage display library Binding assay Insertless phage served as negative control and defined αv integrin phage ligand served as positive control. Results are expressed as mean of triplicate wells relative to binding of insertless phage, which was set to 1. Phage input was e8 tu per well. Experiments were repeated 3 times with similar results. Data shown represented relative enrichment of phage ± SEM from triplicate platings and were reproduced from the graph. NT denotes not tested. Each library was incubated with MOLT-4 leukemia cells for 30 minutes on ice to deplete background and common cell surface binding phage (pre-clearing). 2229 SRXPXXX(0.13) 1 Phage display (subtractive panning) 3 PMID:23143954 NSCLC tumor cells Not determined. Not determined. Not determined. X7 and X11 FUSE5 phage display library pool Prior to NSCLC tumor panning, the negative selection was performed using the normal lung tissue of the patient. 2230 SRXPXXX(0.09) ARRPKLD(0.09) XRXPXXX(0.35) 3 Phage display (subtractive panning) 4 PMID:23143954 NSCLC tumor cells Not determined. Not determined. Not determined. X7 and X11 FUSE5 phage display library pool Prior to NSCLC tumor panning, the negative selection was performed using the normal lung tissue of the patient. 2231 ARRPKLD(0.33) ARXPXXX(0.42) SRXPXXX(0.18) XRXPXXX(0.60) S-R-x-P-x(3) 4 Phage display (subtractive panning) 5 PMID:23143954 NSCLC tumor cells Not determined. Not determined. Not determined. X7 and X11 FUSE5 phage display library pool Prior to NSCLC tumor panning, the negative selection was performed using the normal lung tissue of the patient. 2232 YHDYKLYDTSLK(1) DYKLSDKWAQHA(1) WDYKELDDWTMY(7) D-Y-K-x(2)-D-x-W 3 Phage display (competitive panning) 3/4 PMID:23583685 Anti-SFV FLAG mAb Genome polyprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) For round 1 of panning the phage library was mixed with the mAb. After incubation and washing, protein G was added to the phage-mAb mixture. 2233 EHSPAFIADPLY(1) IHTPAFNVNVRT(1) WNSPVMREFVSK(1) SHSPAFKSTSRL(4) ISPQHPLYSILS(1) AMTQWRATSNMG(1) YLSKGKTHSLHT(1) H-[ST]-P-A-F 7 Phage display (competitive panning) 3/4 PMID:23583685 Anti-SFV V2 mAb Genome polyprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) For round 1 of panning the phage library was mixed with the mAb. After incubation and washing, protein G was added to the phage-mAb mixture. 2234 MHTNYCRTCFLS(1) NGYLCRTCSLTT(1) TGPRFIGSMGPA(1) LQAQKIEVQNQI(1) KLHISKDHIYPT(1) VMRQETPLRVPD(1) HHNPQWIDSQAK(1) H-G-P-Q-C-[IR]-[DT]-[CS]-Q-L-P-T 7 Phage display (competitive panning) 3/4 PMID:23583685 Anti-SFV V3 mVAb Genome polyprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) For round 1 of panning the phage library was mixed with the mAb. After incubation and washing, protein G was added to the phage-mAb mixture. 2235 QQSFMEKWLEYE(7) GVPSFLDMWLLY(4) FNEHHRVHSVTR(1) YVDNSFKRVKAN(1) IVCDDDGRCPNV(1) S-F-[LM]-[DE]-x-W-L-x-Y 5 Phage display (competitive panning) 3/4 PMID:23583685 Anti-SFV V7 mAb Genome polyprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) For round 1 of panning the phage library was mixed with the mAb. After incubation and washing, protein G was added to the phage-mAb mixture. 2236 HPSDYLLTYPFK(1) TWGAWFDFQLHP(2) HPSDYTLPVHPR(1) HPTDYSLSYMYP(4) HPDDYTLRFNRM(1) YNLQSPKSKLFA(1) H-P-[ST]-D-Y-S-L 6 Phage display (competitive panning) 3/4 PMID:23583685 Anti-SFV V8 mAb Genome polyprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) For round 1 of panning the phage library was mixed with the mAb. After incubation and washing, protein G was added to the phage-mAb mixture. 2237 VHNMQKDMVLLQ(6) VHDFKLINMKNS(1) RDYHPMLDMTLP(1) KIALESYNLSSA(1) NGQHAPTVAYSK(1) HKANEWHMLPST(1) SVGLHPPLSRQQ(1) LDLSAFPHTGLP(1) LMKPSYDTAHRP(1) YHTTPQMNKMYS(1) V-H-L-H-P-x(2)-N-x(2)-L 10 Phage display (competitive panning) 3/4 PMID:23583685 Anti-SFV V10 mAb Genome polyprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) For round 1 of panning the phage library was mixed with the mAb. After incubation and washing, protein G was added to the phage-mAb mixture. 2238 FTNTDWLLLFGP(1) VAERSTEETFMG(1) NQTDHLFSTFIS(1) STYWMGLIPAPI(4) HVRAYTAGILFE(1) HYTWMWDELSWY(1) GVTSFDFRRHTE(1) V-T-D-W-[LM]-F-[DE]-[IT]-F 7 Phage display (competitive panning) 3/4 PMID:23583685 Anti-SFV V11 mAb Genome polyprotein Not determined. Not determined. Ph.D.-12 phage display library (X12) For round 1 of panning the phage library was mixed with the mAb. After incubation and washing, protein G was added to the phage-mAb mixture. 2239 HAIYPRH(4) GETRAPL(2) SILPYPY(2) SMYGSYN(1) KLPISSK(1) KDPGWSG(1) STASYTR(1) IQSPHFF(1) YLTMTPT(1) GPIIPRN(1) HAIYPRH 10 Phage display (subtractive panning) 4 PMID:23541699 Neural stem cells, NSC Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) After a negative selection against the plastic cell culture dishes, four rounds of panning against NSC were performed. 2240 KLPGWSG(9) HAIYPRH(1) GETRAPL(2) ALTPWAF(2) GKPMPPM(2) EQRGAPR(1) YSIPKSS(1) TDSLRLL(1) KLPGWSG 8 Phage display (subtractive panning) 4 PMID:23541699 Neural stem cells, NSC Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) After a negative selection against the plastic cell culture dishes, four rounds of panning against NSC were performed. To understand if the KLPGWSG peptide effectively binds to NSC we performed FACS analysis using antibodies recognizing NSC and the peptide functionalized with the fluorochrome FITC. These results strongly indicate a binding of our peptide to NSC. 2241 YLTQKPSPPYQG KIHYWPSTPTLT WTCQKAPCVARV FKMPQTMVMRTK GFNSAYKPQMRD LPYPQHPGSLGR FPPSWLAASNRP LPPQHPWDNSKH HSPVLKTPSTHA YSWHTDPKTLKR 10 Phage display (common panning) 3 PMID:23711778 Diamond-like carbon, DLC Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) After three rounds of biopanning against DLC, 28 phage clones were randomly picked and their peptide encoding DNAs were sequenced. 18 peptide sequences were novel. Eight of the sequenced peptides, were longer than the 12 amino acids that would nominally be expected to be found in the PhD-12 library. 2242 VASPERPSPAFP(7) EASPERPSPAFP(7) AAPLGTHPSMHP(7) LPPLGTHPSMHP(7) SSATPNRLTWLL(7) YSHVHALSTYAR(7) VASPERTSPAFP(6) 7 Phage display (subtractive panning) 4 PMID:20211209 Anti-DENV2-NS1 pAb Non-structural protein NS1 Not determined. Not determined. Ph.D.-12 phage display library (X12) A negative selection procedure was performed in which the amplified phage was pre-incubated with the bead in the absence of the antibody. The supernatant was then reacted with the antibody in a positive selection. Twenty-five of 30 selected phage clones had significant enhancement of binding activity to DENV2-NS1-specific antibodies. 2243 RKKTPASRRPMR DLKPPIPSQSGP SPPYTYLPQKVQ HMGADPLQKRPS DLFARRPASSDW HFQMFHRPSGPQ SHQMAWPLEATS WHWTNWGKTSPA QHQHVNTLPERP HNWLYYLRTSTA HWRLPLLTSLMA NLTSLTQGSAML 12 Phage display (common panning) 2/3 PMID:19708689 Vitamin B12-binding protein Protein tonB Not determined. Not determined. Ph.D.-12 phage display library (X12) From BtuF-targeted assays, 120 unique peptides were affinity-selected from the Ph.D.-12 library. 2244 CHPTPTDIC CVHPYESHC CDSPLSNLC CHLTSMQMC CHLQTSSSC CWTPSPATC CYSTAVREC CTMRAVSAC CMASLSRSC CALKTNQPC CSHTQPYLC 11 Phage display (common panning) 2/3 PMID:19708689 Vitamin B12-binding protein Protein tonB Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) From BtuF-targeted assays, 81 unique peptides were affinity-selected from the Ph.D.-C7C library. 2245 TPLSLAPADQWL WVLAPADRARSL FDLAPADRNWGS QDLTLAPADYLA SMQAHLRLAPAD TQHLTMAPADSK MHQTLSPADLAT ALLCDWAPADCS 8 Phage display (common panning) 3 PMID:18653801 Anti-FhuA Fhu4.1 mAb Ferrichrome-iron receptor Not determined. Not determined. Ph.D.-12 phage display library (X12) 2246 SQLGVKYHMPGS QTGGLGVKYFRV QTQTLGVKYFRE YAQYGVKYKTET YFNDVQLGVRYH LPSTISVKYHST 6 Phage display (common panning) 3 PMID:18653801 Anti-FhuA Fhu6.4 mAb Ferrichrome-iron receptor Not determined. Not determined. Ph.D.-12 phage display library (X12) 2247 FPVHNLGVRYPG DPDQLGVRYHKA ELGVNYYSATSR SQTDLSVKYYNS 4 Phage display (common panning) 3 PMID:18653801 Anti-FhuA Fhu6.6 mAb Ferrichrome-iron receptor Not determined. Not determined. Ph.D.-12 phage display library (X12) 2248 HVTTTFAPPPPR(4)[0.316 ± 0.004, 1.979 ± 0.007] SVVPSKATWGFA(3)[0.303 ± 0.002, 2.220 ± 0.014] FKPSSPPSITLW(3)[0.327 ± 0.002, 1.645 ± 0.025] T-x(2)-F-[AK]-P-x(2)-P 3 Phage display (common panning) 4 PMID:21176936 Aminopeptidase N Transmissible gastroenteritis virus, TGEV Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Binding of the selected phages to pAPN was measured by ELISA. Ten selected phages and an irrelevant phage were incubated with coated pAPN. OD490 value of individual phage from three parallel wells is reproduced from the graph and shown as the mean ± SD of triplicate determinations. The absorbance of the irrelevant phage was 0.078 ± 0.011. Besides, the binding ability of the identified peptides to pAPN was investigated in ELISA by coating the pAPN as target protein. The TGE virion and IBV S protein produced from the same expression system were used as positive and negative controls, respectively. The OD490 value was reproduced from the graph and shown. The absorbances of the TGE virion and IBV S protein were 2.676 ± 0.014 and 0.472 ± 0.052, respectively. The chemically synthesized peptides block cell infection by TGEV through competition binding the viral cellular receptor. 2249 LLADTTHHRPWT(12/30) EHMALTYPFRPP(5/30) 9 Phage display (subtractive panning) 6 PMID:17125317 Single-walled carbon nanotubes, SWNTs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The phage library was preincubated with a graphite suspension to minimize the isolation of graphite-selective phage. Panning experiments were performed by mixing the recovered phages with the SWNT suspension. Biopanning was repeated six times with increasing Tween-20 concentrations (i.e., 0.2, 0.3, 0.4, 0.5, 0.75, and 1.0%). A new motif, X1THX2X3PWTX4, where X1 is G or H, X2 is H or D or null, X3 is null or R, and X4 is null or K, was identified from two classes of phage-displayed peptide libraries. 2250 ETAPLSTMLSPY(17)[1.924 ± 0.074] SVSVGMKPSPRP(14)[0.772 ± 0.028] TLHPSVLSYVLK(13)[2.025 ± 0.194] EVFWPLNAPRLL(11)[2.293 ± 0.179] ASTNVFARPMYL(8)[0.571 ± 0.063] APKYSLSDLYLN(7)[0.884 ± 0.153] TPPPRDASLSRW(7)[1.001 ± 0.096] SWQITYPISPRS(5)[1.628 ± 0.084] TALPNHWSDASP(4)[1.874 ± 0.123] SSFPQVIPLDYL(4)[2.293 ± 0.196] TDSYHVASARQP(4)[1.633 ± 0.327] YTFMPELTTPRT(4)[1.554 ± 0.074] NSFNYAPLLMPR(3)[1.633 ± 0.090] SGFFEPQHSHPL(3)[1.298 ± 0.055] TTFSPPSPLSSI(3)[1.237 ± 0.265] KTAPTPYALVHD(2)[1.385 ± 0.127] FNNHYPAAATYP(1)[0.720 ± 0.164] SWQIPYPISPRS(1)[0.910 ± 0.122] YTTWPFTSLQLD(1)[2.204 ± 0.401] ETAPPTPYSVMF(1)[1.940 ± 0.095] TTFNPLYLRLDT(1)[2.473 ± 0.090] TSPYHLLHAHLQ(1)[1.792 ± 0.084] SSFVALSISPSM(1)[2.393 ± 0.429] SPQTDGLVSTPS(1)[1.132 ± 0.166] SNFMHNTRIWSH(1)[NT] STYSHPLSLRPD(1)[NT] AWTWVLPSSIRA(1)[NT] AFMETTSQNAWL(1)[NT] QIEKISQHLDMH(1)[NT] TWNQPYIPPLYP(1)[NT] YASPPNPSLRLT(1)[NT] YHGLTPVRYVSV(1)[NT] ANLSSHSSPGDS(1)[NT] TTLQFTGQTNKT(1)[NT] HNIGTWGPKSHL(1)[NT] QIEESFVRGHTT(1)[NT] YTFDPQIRPAGL(1)[NT] YERSILPFSHVF(1)[NT] YPSVTFPTQTLL(1)[NT] SPWYMTPSPNTA(1)[NT] HISVINYTTKIS(1)[NT] MNVTVSGRLSGP(1)[NT] TIPYPFSLLNNP(1)[NT] PFLYSQVAWRS(1)[NT] YQEETPASSFSR(1)[NT] NSSQLAPYTTHR(1)[NT] HNGLPNFFQTRL(1)[NT] EAAPNFYPPLTF(1)[NT] DLFQFAFPLNTI(1)[NT] FTFSYAESVSYF(1)[NT] 50 Phage display (subtractive panning) 4 PMID:23792224 MDR GC cells SGC7901/ADR or SGC7901/VCR Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA In phage ELISA, unrelated phages (URPs, the amplified phages from original phage peptide library) and PBS were used as negative controls. Absorbance value was determined. Data were reproduced from the graph and expressed as mean ± SEM (n=3). The absorbances of URPs and PBS were 0.239 ± 0.028 and 0.093 ± 0.043, respectively. NT denotes not tested. Whole-cell subtractive screening from a phage display 12 peptide library was performed on MDR GC cells with a drug sensitive GC cell line, SGC7901, and immortalized gastric mucosal cells (GES) used as control. After four rounds of panning, 200 phage colonies were picked randomly and identified for MDR reversal capability using the MTT assay. GMBP1 (ETAPLSTMLSPY) could inhibit the tumorigenesis of gastric cancer MDR cells and might have the potential to re-sensitized gastric cancer to chemical drugsin vivo.