201 YPRYDYHDW WSHYSSEPL PSMSYYTTW YQYIETSMQ CDWELCYAE YYYSTPSVA NYYDRYAIE VADLTYYAW AHNYYPREW 9 Phage display (common panning) PMID:15265865 Synapsin-1 Ras-related protein Rab-3A Not determined. Not determined. pVIII-9aa phage display library (X9) Antibodies raised against the peptide YQYIETSMQ (syn21) specifically recognized Rab3A, a synaptic vesicle-specific small G protein implicated in multiple steps of exocytosis. 202 EDPSHSLGLDVALFM(9)[0.428 ± 0.043][5.0 ± 0.4] QTPETLTLFPPNWMN(7)[0.399 ± 0.017][15.4 ± 2.2] EANAPLDMGVFFEHN(3)[0.371 ± 0.074][16.2 ± 1.5] NNNQLDCLLFVRQCK(1)[0.150 ± 0.060][112.0 ± 21.0] RLTSPADMGSFLFNL(1)[0.250 ± 0.051][227.0 ± 18.0] RTTLGHHLDFTLWTA(1)[0.147 ± 0.024][60.0 ± 5.4] 6 Phage display (common panning) 4 PMID:15833876 Anti-GD2 monoclonal antibody 14G2a GD2 ganglioside Not determined. Not determined. X15 phage display library ELISA,Flow Cytometry For reverse ELISA, 14G2a mAb-coated wells were incubated with positive phage clones (0.5e12 transforming units), washed and incubated with HRP-conjugated anti-M13 mAb. After a final wash, the reaction was developed with the HRP substrate and quantified by reading the absorbance at 450 nm in a plate reader. The wells incubated with negative clones remained nearly colorless. The absorbance was shown. Besides, synthetic peptides representing the individual phage families were analyzed for the ability to inhibit the binding of 14G2a mAb to GD2-positive IMR-32 neuroblastoma cell. Varying amounts of the competitors were incubated overnight with 14G2a antibody (1 μg/mL). The mixtures were transferred to IMR-32 cells, and the inhibition of 14G2a binding to cellular GD2 was measured after staining with FITC-conjugated secondary antibody by flow cytometry analysis. The IC50 (μmol/L) was shown. 203 FRMDFDYLYPSLP LNFMIFYLSLNPW FSYSVSYAHPEGL SVAFYDYLPTDLP LSFSDFYFSEGSE FAPMKSYGVSLPP LFGPIEYTQFLAN LFDAYWYSDTAMS PASLELYENLVAG GENFCPYSFFGCG YLSLHAYESFGGS FFGFDVYDMSNAL FYMPFGPTWWQHV LPHLIQYRVLLVS GFAWSSYLGTTVH FLSFVFPASAWGG FFPSSWYSHLGVL FFSFFFPASAWGS 18 Phage display (common panning) PMID:16873017 96-well immunoassay plate (polystyrene, PS) Not determined. Not determined. Not determined. X6PX6 and X6YX6 phage display library pool 204 FAQDLQG(16) WNTKASY(3) SEHSVRL(3) SAHPLYT(3) FNQDIMG(3) AHPQLAT(3) EVTSTAF(2) YTFTQDN(2) LNSKGPI(2) RPLRNWP(2) FSQDLHP(2) FNQDLHS(2) TNAPRMA(1) TSLQHLM(1) SSTPALL(1) MGHKYSQ(1) SPTSGHT(1) YNAPPQL(1) LPRPFTL(1) AVVPKSL(1) SLLNSPV(1) EPWYYSP(1) TFVRINP(1) AWYSLTW(1) MPFGDHH(1) ITNGPPM(1) SLAMYQY(1) DSRNSAT(1) LTLPAAL(1) TSLQHLV(1) SHTPALL(1) IPLQSLS(1) LHAGMPP(1) LAQRFST(1) TIANLSL(1) AEPVAML(1) SGFVFQQ(1) SISNLLL(1) YTFRQDM(1) 39 Phage display (common panning) 3 PMID:16054642 Anti-M. hyopneumoniae polyclonal antibody Mycoplasma hyopneumoniae Not determined. Not determined. Ph.D.-7 phage display library (X7) 205 CQTALNRFC(9) CSPNQLKLC(7) CLPSLSSGC(6) CKNLGVKEC(6) CSVRTDTHC(5) CPKPEQGRC(5) CNPFDHASC(4) CPVQNPRYC(4) CSPGDSPEC(4) CPQFLTNIC(3) CLHPWHKNC(3) CPQSLVRIC(2) CKQFLNMRC(2) CTLPSLRHC(2) CLFDRNNSC(2) CPQSLRHIC(1) CPEKMLAWC(1) CNPFNHSAC(1) CLQRLQQHC(1) CPKPEQLLC(1) CPQFLSNIC(1) CNSWLNMTC(1) CPQQLTRIC(1) CPQSLSRLC(1) CPQMLSRIC(1) CEGPAPEEC(1) CWLNMSKDC(1) CPQHLHRVC(1) CNPFNHQLC(1) CTLDRLKQC(1) CNPFNHSGC(1) 31 Phage display (common panning) 3 PMID:16054642 Anti-M. hyopneumoniae polyclonal antibody Mycoplasma hyopneumoniae Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 206 CPTSLLWWC CQRSPHSTC 2 Phage display (common panning) 5 PMID:15598577 Galectin-1, Gal-1 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 207 CQSPSARSC(6) CGAHRLHQC(3) CYSFPHTTC(1) 3 Phage display (common panning) 5 PMID:15598577 16 kDa beta-galactoside-binding lectin Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 208 FDLSEALVPPHR(7) ELDLADALPVLA(5) LPFNLTDALPWW(1) WHVTPFTLYDAM(1) SPGNFSLADALL(1) VVPFSLRDALAI(1) SSSYPLLLEALP(1) NTLGTFSLYDALG(1) PKDFSLFEALLG(1) CSAYALDALLPR(1) [AL]-x-[DE]-A-[LM] 10 Phage display (common panning) 4 PMID:15979564 Calpain-1 catalytic subunit Calpastatin Not determined. Not determined. Ph.D.-12 phage display library (X12) 209 ANDLAHWEPMSF(1) MCRLCHWPSASR(1) HSPTFHGLPPGL(1) AHLRPAPGDPWF(1) KLLPNSLWARTP(1) KLLPPPTLGRST(1) KMMPELNPSVVH(1) QIVKQNPNAVLS(1) QLPHRIPLIPDQ(1) 9 Phage display (common panning) 1 PMID:15927323 Anti-P25-LHRH polyclonal antibody Luteinizing hormone-releasing hormone I Not determined. Not determined. Ph.D.-12 phage display library (X12) 210 MYTQQRHWSEGL(1) TKIQEELAHWSP(1) DSSSMLHWTYSL(1) HWEYRVRTYPQT(1) APSLLHWGPSFW(1) GKLVWSDSFSTA(1) KLIPNSSLHSDS(1) SVPPKTIPNHSL(1) KLTPSQSLLGAT(1) TSKLTPGHSILN(1) SKLLPNYALTLN(1) STLKMLPHSRFF(1) MKVTPNRSLSTT(1) SKLMPNNSLWLY(1) KAIPGSWWQLWS(1) AKLPANWHLMLP(1) 16 Phage display (common panning) 2 PMID:15927323 Anti-P25-LHRH polyclonal antibody Luteinizing hormone-releasing hormone I Not determined. Not determined. Ph.D.-12 phage display library (X12) 211 KGIPNSMLLELR(2) KMSPTINSLLWQ(2) QYEHWSEGLT(1) GELDHWSGLPPH(1) DAPHMRHWSWAL(1) RPPLSELSHWHP(1) KLIPNSSLLMGA(1) HKMIPNHVLWRP(1) KILPNQSLFHVG(1) GKWLPNSSFMQH(1) INAKMLPNHFLT(1) HKLTPNWSLSFT(1) KITPNYSLVSGP(1) KLSPNAMLLEQP(1) KLSPNMTLLGPP(1) YAKQVPNTSLVM(1) NAPKLVSNWFLA(1) 17 Phage display (common panning) 3 PMID:15927323 Anti-P25-LHRH polyclonal antibody Luteinizing hormone-releasing hormone I Not determined. Not determined. Ph.D.-12 phage display library (X12) 212 TNTHWSYMLPSA(1) NVVSVGHWSYYV(1) TLHEHWSRALLE(1) HWSSMVHSPMP(1) SFGTHWSDLLSH(1) ATTMHWSDQLEA(1) HWSTRVHHTPNS(1) QTITTMHWSSSL(1) HWSDRVHNRLTL(1) HWNYYKDTLPIQ(1) KSLPLESFLAPW(1) FNDKHIPNSRLL(1) STFPKLPPGASL(1) QILVETQVKQTP(1) TQETTISGRVLP(1) 15 Phage display (common panning) 2 PMID:15927323 Anti-P25-LHRH polyclonal antibody Luteinizing hormone-releasing hormone I Not determined. Not determined. Ph.D.-12 phage display library (X12) Two rounds of panning experiments were conducted: the first with acid elution and the second eluted by LHRH peptide. 213 ASYSDLLYSSFL(15) ASYSDVLYSSFL(2) SDKLLHSVRPPV(2) 3 Phage display (common panning) 2 PMID:15927323 Anti-P27-LHRH polyclonal antibody Luteinizing hormone-releasing hormone I Not determined. Not determined. Ph.D.-12 phage display library (X12) The panning experiments were conducted using the generic acid elution procedure. 214 KLLNSVPLIHGS(8) NLLKSSNTLPFD(1) DSLHWSYMLLPS(1) 3 Phage display (common panning) 3 PMID:15927323 Anti-P27-LHRH polyclonal antibody Luteinizing hormone-releasing hormone I Not determined. Not determined. Ph.D.-12 phage display library (X12) The panning experiments were conducted using the generic acid elution procedure. 215 KLLNSDALPVVA(13) KQLNSVPQELSF(2) GVKQLNSVIKPG(1) 3 Phage display (common panning) 3 PMID:15927323 Anti-P27-LHRH polyclonal antibody Luteinizing hormone-releasing hormone I Not determined. Not determined. Ph.D.-12 phage display library (X12) A total of three rounds of panning experiments were conducted: the first two with acid elution and the third eluted by incubation in TBS buffer. 216 WPAALFESNRPD(18) DIHWTSMMVRPT(1) 2 Phage display (common panning) 2 PMID:15927323 Anti-P35-LHRH polyclonal antibody Luteinizing hormone-releasing hormone I Not determined. Not determined. Ph.D.-12 phage display library (X12) The panning experiments were conducted using the generic acid elution procedure. 217 CDRASPYC(4) CDLASPWC(4) CDRATPYC(3) CDRASPWC(3) D-[RL]-A-[ST]-P-[YW] 4 Phage display (common panning) 3 PMID:16923020 NeutrAvidin Biotin Not determined. Not determined. CX6C phage display library 218 CDRASPYC(12) CDRATPYC(7) CDLASPWC(3) CDRASPWC(2) D-[RL]-A-[ST]-P-[YW] 4 Phage display (common panning) 4 PMID:16923020 NeutrAvidin Biotin Not determined. Not determined. CX6C phage display library 219 CDRASPYC(20) CDLASPWC(7) CDRATPYC(6) CDRASPWC(2) D-[RL]-A-[ST]-P-[YW] 4 Phage display (common panning) 5 PMID:16923020 NeutrAvidin Biotin Not determined. Not determined. CX6C phage display library 220 CSSKSYRPC(5) CNKPKNASC(1) CPHLPNSTC(1) 3 Phage display (common panning) 4 PMID:16581206 Anti-leptospira monoclonal antibody P1 (2C3D4) Leptospira Not determined. Not determined. CX7C T7 phage display library 221 CTPKKSGRC(2) LTPCDSNKSGRC(2) TPCSKKSTRNC(1) TPCSKKDPRNC(1) CRKKNTNNC(1) CRKSKSASC(1) CTTNSKRKC(1) 7 Phage display (common panning) 4 PMID:16581206 Anti-leptospira monoclonal antibody P2 (8C6C4A12) Leptospira Not determined. Not determined. CX7C T7 phage display library 222 LTPCDPNTNSFC(5) CGIPGTPDC(3) LTPCDPNTNNFC(1) LTPCDINTNSFC(1) 4 Phage display (common panning) 4 PMID:16581206 Anti-leptospira monoclonal antibody P4 (2D2A12G3) Leptospira Not determined. Not determined. CX7C T7 phage display library 223 CKSKKSSSC(5) CNKKDPSSC(1) TPCSKKNPGNC(1) CKSKLVRC(1) CESNKSSC(1) 5 Phage display (common panning) 4 PMID:16581206 Anti-leptospira monoclonal antibody P5 (15BC2G1) Leptospira Not determined. Not determined. CX7C T7 phage display library 224 LTPCDAPPSGIC(9) CGRTRVTRC(1) 2 Phage display (common panning) 4 PMID:16581206 Anti-leptospira monoclonal antibody P6 (1B4D3F10) Leptospira Not determined. Not determined. CX7C T7 phage display library 225 CFATVERAC(7) CLLTVERAC(1) LTPCDSDWPVSC(1) 3 Phage display (common panning) 4 PMID:16581206 Anti-leptospira monoclonal antibody P7 (16B1C9H7) Leptospira Not determined. Not determined. CX7C T7 phage display library