2201 WHWTYYW(9) WHWRAWY(3) DAFAWVP(2) ITYGFNP(2) WHWRAFF(1) AYWPSPH(1) TPLAQYF(1) LSFPYPR(1) SSMHTWR(2) WKSHQPP(1) THSLRFP(1) MPPQQYP(1) YPQPKHL(1) AHHSNLY(1) GPSVYSI(1) VWLKSAP(2) KYPPVHL(1) SWAQLPI(1) NPHKGMA(1) MRPPSPL(1) TTPTSRV(1) TLTNQLS(1) EPPPAQR(1) HHRIQTL(1) QASPQLS(1) GGASPIH(1) AHHMSDR(1) LPPNPTK(1) LPALQTV(1) WHWTYYW 29 Phage display (common panning) 3 PMID:23577599 Crystalline cellulose nanowhiskers, CNWs Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) From the single phage biopanning and ELISA analyses described, the binding specificity of the phage-displayed peptide WHWTYYW suggests that this segment might be a major determinant for interactions with crystalline cellulose substrates. 2202 YPHFHKHTLRGH(9) YPHFHKHSLRGQ(1) DHKPFKPTHRTL(1) FHKPFKPTHRTL(1) QSSCHKHSVRGR(1) QSSFSNHSVRRR(1) DFDVSFLSARMR(6) 7 Phage display (common panning) 3 PMID:23861727 Metuximab CD147 Not determined. Not determined. Ph.D.-12 phage display library (X12) 2203 KHSPVHI(4)[3.013 ± 0.186] LPLTPLP(1)[0.648 ± 0.081] IHSPVHI(4)[NT] YHSVVHI(4)[NT] HHTGVHL(2)[NT] NPLRVSL(1)[NT] NQDVPLF(1)[NT] HDTPAPP(1)[NT] MPSNTVR(1)[NT] SYSSFHR(1)[NT] SPTHGHD(1)[NT] THSIVHP(1)[NT] LHSPVHL(1)[NT] KHSVVHV(1)[NT] KHSPVHI 14 Phage display (common panning) 3 PMID:12413654 Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA The ELISA plate was read at 405 nm and mean values and standard deviations were taken for the ten coated wells. Data were reproduced from the bar graph. The OD for control peptide NRPDSAQFWLHH was 0.599 ± 0.057. ELISA results demonstrate the phage borne peptide KHSPVHI, representing the consensus sequence, binds Par17 with the highest affinity and specificity when compared with other phage display derived peptides. All other peptides are non-specific and weak binders as the measured OD is comparable with the non-specific control peptide NRPDSAQFWLHH. The peptide consensus sequence XHSXVHΦ was enriched , where X can be any amino acid, and Φ is any hydrophobic residue. 2204 THSPVHVLAEHI(5)[3.273 ± 0.225] YHSDVHCANTCN(5)[3.351 ± 0.120] SHSVVHVTKNQY(3)[2.410 ± 0.172] WHTGVHISAKPG(2)[3.783 ± 0.120] ELTNAQIKLLWE(1)[2.090 ± 0.172] 5 Phage display (common panning) 3 PMID:12413654 Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Phage ELISA was utilized to measure the ability of the phage-borne 12-mer peptides to bind Par17. ELISA 96-well plate was coated with Par17 (10–20 μg/ml) and ten wells were coated. Absorbance at 405 nm was measured. Mean values and standard deviations were taken for the ten coated wells and shown. Data were reproduced from the bar graph. The peptide consensus sequence XHSXVHΦ was enriched , where X can be any amino acid, and Φ is any hydrophobic residue. 2205 SSFPPLL(8)[0.502 ± 0.163] LPLTPLP(5)[0.648 ± 0.081] GKPMPPM(1)[NT] 3 Phage display (common panning) 3 PMID:12413654 Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA Phage ELISA was utilized to measure the ability of the phage-borne 7-mer peptides to bind Par17. ELISA 96-well plate was coated with Par17 (10-20 μg/ml) and ten wells were coated. The ELISA plate was read at 405 nm, mean values and standard deviations were taken for the ten coated wells. Data were reproduced from the bar graph. The OD for control peptide NRPDSAQFWLHH was 0.599 ± 0.057. ELISA results demonstrate that these peptides are non-specific and weak binders as the measured OD is comparable with the non-specific control peptide NRPDSAQFWLHH. 2206 SSFPPLL(13)[0.502 ± 0.163] LPLTPLP(1)[0.648 ± 0.081] GKPMPPM(1)[NT] 3 Phage display (subtractive panning) 3 PMID:12413654 Par17-GST Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA Phage ELISA was utilized to measure the ability of the phage-borne 7-mer peptides to bind Par17. ELISA 96-well plate was coated with Par17 (10–20 μg/ml) and ten wells were coated. The ELISA plate was read at 405 nm, mean values and standard deviations were taken for the ten coated wells. Data were reproduced from the bar graph. The OD for control peptide NRPDSAQFWLHH was 0.599 ± 0.057. ELISA results demonstrate that these peptides are non-specific and weak binders as the measured OD is comparable with the non-specific control peptide NRPDSAQFWLHH. The phage library was incubated with Par17-GST in a positive selection. Starting from the second round, a negative selection was performed where the phage was incubated with the resins (in the absence of target). 2207 IVLPTWL YSTRHTD ADRSPLW YSSVHID YTSQTPR QTSITMY HRVLWHQ SFTYPSW ELCFKCS SDRSALW VSPGPTG SEWLWDH QCTLCPR YATPWQM TLIDPSR VLSDPPP RIPMQDL YVFPTWS ERMNHNP FYLPPWS ASQDITP IHTHPWY SHGSVHA GLGSVKP YIWPTWA VGFRLGL STTIALF SSPPKRL YAPPYQR STSELYG YDMSLIG QYMNQHA YDRSVLW HFVWTRS IELRGIY SDTTGLP EFTYPSW GWMMRFS QDRSILW ERVMSMM LAAKVHR MLDPRWE NFMYPSW TPMKISH AQSNSMH ILPVHVS VPPGTII QGLGTRG EPKTKHI HLSYGLN YIFPTWA YVFPTWM QFMSQVY SSGQHAF FIWPTWS TGSPQAR YGVMAPH RNDLPYT GQWSRIN GNNLVRT SSAQSSQ FKFTFAL SRYVDGY VPSRESW QLAMTYV MMNPIRN GELPGTA WIGIDTV LSINVVF EEWNWLR LSWTGKS GLFREGW MAQSSAA QYMYQLP DFWHHLR ADRSALF VPSFVPL QCLLTCA DSPLHAT QTYRPSY ANFWGES NFYLPTW WPISFLI YVFPTWM YSTNHID WKSPVLM VFGVHAH FNQPTWL HHSPMIG SLGKTVA AWPRHAA GIFKYFS NFVPPSW MGDIKHV TGTDATF CYMCQSL QYMFQTS TQGYATS NSGLHMF YPHRQVP YSRLHLD LPVERLN LLAVDAL HSPQSIA SGMTVLI WDPHWPK VVTIPYP LATRSSS GPHFELR RVLPSDE ETSSSIA HLQSSYQ SIRLHLG NGYINAM FYLPTWY EFHLGES MSNRDSE FTLPSWL KVTTMKP APPLSNL GSAQESH HHGAKHF TEFDVDH ITSPWPG VAKTYTS WGQNQSS LLGNIPP TSTTSRG YPFGVWT NHHAYPT ELALWST SWLSIFG TDNSARP HTAHVVT TVCCQTA FWFPPWE KFIIKAQ SVELQFG FYWPTWV MTQDITI SPLPWWQ WGGTLAR FSTVSQR RLVAQWP WTPMHDP GVQKASN DRLHMEP QFFWPAG WSSGVIF SLALMMR ETHHAHR LSHFSIV FIPRLSY SVTQSVI LMTPIMK KFVVKFN DHDRSWF TQHSYFL GNTYGFH IVMPPGP VSQSHLQ FRIPMID IFARTTM QHRALPT AVNTFML KGAYYQS VGLSIHI DKLYVTL TVRTGST GQDITAQ ADRSIMW THHHPDE QQFKSMS LIPTNTV LGTILHQ VHTGGLA IKISYPS MPGRGGV ATFGVWT NHVQGLP QFMSQMA IKLQPSV ETTQRPM GPYHAQP FTMPPWE FPFKWLA GASRAPA ADHVSHG VTSNFSH NSLTSYT MLERFLS HYFRING TFTYPTW HTHWFHT DPKGAYT YSSMHID IGQLVKL DSEHMTS WTWPSWA YTSQTIR KFWQGVT SKQDIIA SGMDRHE VTANTRE FSFTLTV AKVAITI FVRPTWH VSGTHML SPFFWEL TARSVWI VSTRHLN WNAPVPN NLDANLV KPNFDLH GAAGDAY TMTRNFM LRLNITW RIPMNDT YTGLQTQ CIFTCHD MDRSALW TISKWEE DRYVSWH EMRTERV DWNKAGD CHLYCEA GEWEYDH WNGMIGM EQTQARV YSTTHVD MPAHLNE SPFKWLA HISWRMM QEYHLAS LLGGAVA RIPMTDE NICIRCP QNWVPFN CYMCYQT SVVLNWN QFMYQPV YTTLHRD LANMSLL FVEPRSH SSGELWN MTDSHVS SYQMDYA AAKLLPH GRIGLQV QYMN*NF MPALVKF NLTHRGP LSLNMHD VLDFTLA 254 Phage display (competitive panning) 2 PMID:23826227 Anti-PAP sera sample 1, PAP1 Prostatic acid phosphatase, PAP Not determined. Not determined. Ph.D.-7 phage display library (X7) The phage library was mixed with mouse or human serum. After incubation and washing, protein G was added to the phage-antibody mixture. 2208 AWFYPPW YVHPPWW LGYPWMQ NFRLPPW NPQWQTS YELPPWM FVRPSWI AFFGPSW WTRPPWE TFLPSWA YVFPTWS APPLFTP WTLPSWE NFQMPSW QWWNHEI GCCMHKN YINPSWW SLVDIWV FSLPPWA YVWPSWM GSLHFLV FTSPSWQ TYWPFPR SPLNIAR SYPWLIN KDLRDWF YVNPWEP FWEPPWG GDMHRVM TQVRPPW SFLPPWM WNWPTWE DLYYGRV GLWADNE FNSPSWS GFMAPSW LGSAWDV IIMPSWE GLNAALF APQMHLP TDGKSHL YVQPSWL WEKINHT AP*MHLP YLLPPWE HYLPSWH FSFPPWS LVLPSWL EQFYPAM ILWLLLW NYWPFPW VVWDRNP GYPWISN ESLNSFA TMWPFPS FVRPTWH HPLQVFV YFLPPWI NVLPPWL FELPSWM FVWPYWQ IVLPSWW APPMHII EFFFPSF VYAHTWL FVSPSWK FSMPSWQ ITLPTWL GFKEPSW TFRAPSW KAPHLES HFAFPSW FHRPSWD FTHPSWS VFVEPSW GGKLPLN SQLMPSW IWLPPWF FLLPPWN GWFLPSW WFINPTW AWWAPSW SFVKPTW KPVDLTA FRQPSWY AQWYPPA WVLPSWM FFDPSWN GFTLPSW FDFPPWA FNEPPWH VWMPSWA HEPTVMS RMVLPSW LTDHQRY NFILPTW ANLSQVL GFFLPTW SDHRHWI RFMVPSW VQMPASK HSLERLA FALPSWH GDYIPVK EDTKTSH FVFPSFH WTQQWYY LVYGDER VPSGKLH VVWDRHL ILLPSWE TFSYPTW VHNTPYQ QWWNLEI WHDPSWV DFIIPSW YPWINQP RFMDPPW FNPPSWG FINPTWA TWFAPTW VILPSWY VPGHHLM SVPSSEV FVMPYWA FKWPPWN LIYPSWA KSADHYY FSLQSFA SFMFPPW SFYEPSW TFTLPTW HSLEMLW SFQSPSW SYLRDLW HTHASGG AFRMPSW LVWDPWP FTQPSWQ RWVSPPW VLPSWAQ LIPVALT YWLPPWS EQFYPNW HVKAVET YEMPIPL GASRAPA NFTRPSW VVWDRSP MVVSSAN FTQPPWR FLVPSWH WFRMPSW QMEALMP SPPLQMF EFRSPSW IFSFPSW EVFWARL RPVDTPQ HSLVLPN QWWAGES KFQLPSW TFNPPPW YPWMQFS FLWPSWT QFRLPPW FNPMWQW FHYPSWR FTEPTWA VPRWFPP YYFPSWM YIVLPPW FFSPSWL RADYHAY YLGNYMI HLSTKGV TWWMREL FYSPSWT WPIFWH* QLTLPSW YSGQFNW NFAFPSW HFNEPSW ISTHYYL IPLNYNV FISPSWT NNNPQWQ FVAPSWN LVWPTWA WSWPSWV SLIQPSW QVLPTWS TYVYPPW TNPMW*M HSLEDVY LVNPPWA HMMRTNH LDFPFTS HVLPPWS WSEFRTL HSLEAIH SHYQYPD HKGFPMS LYLPPWQ LNLPTWM FLNPSWQ WFMPPWD DYLQNNA HSKPYLT FIRPTWG YHWATWE HFTMPSW YPEWGHN DQPWHWI YDMSLIG EFISPPW QVLPSWA HALEMIA WSTYRTA VTNFKTN LILPPWL WSWPSWN TRLYITD HIGQPRS VEDYMMR WQRLPSW YVRPPWS MVNPTWM LLWDPFP NVSLQMF QWHEPSW LWWSPSW NDAFASM YIMPSWS FEWPSWG FMNPSWK FIKPSWV HHIDSLW WSLHRTG YNWVQQS FRMPSWT NTVVLHL YVLPSWM HSLTIRN TYPWVTQ NWLPTWE FLNPSWE NWMWDAN AFIGPPW EIAMTTL LHHGVPV INLPSWM WTYPPWG HFTHPSC 254 Phage display (competitive panning) 2 PMID:23826227 Anti-PAP sera sample 2, PAP2 Prostatic acid phosphatase, PAP Not determined. Not determined. Ph.D.-7 phage display library (X7) The phage library was mixed with mouse or human serum. After incubation and washing, protein G was added to the phage-antibody mixture. 2209 RVIAPSD WPVPYPL QWDDHW* QSPYPLN WPAREYF TLSGSRF TPQPYPL YETWEHR THSIFVY DWRNHWL AVSIVKR QWDDHWY RHEWLLH WLTPYPL AIPRLEE YHDAYPM YEGSIPH SPSPYPF VPSAYPL QANNTHM FWNTEML YNPWTHW NFRILSI THYRVNS KQPLHWY DLTPYPL DQPMHWF FSWRMMT DHVSWHY QWEDHWQ QDPYPMN DHTSWHP SGRIIGS SHVSWHT MHRMELR GHEPYPL DVPNWRT MLTPYPL YPNTDMT AWPRHAA VTITVQR CLAGGRP NPSAYPM THVSWHT DMPSQWG LRQPATG DMPLWWT EHPSWYP GGLIIGM ASNANLR WMPMRDR YPPFTDP GKPCEHC EFRLPQL TPQPYPL FNVGVKP QCDDHW* NHTPYPL LSKFPTS TVAQATS YRPSMNS SWAIRIY NWLKVGT TAWSVMK TVEYITN TFVSMPP RLPMVEP NALTGSA HYKPVYP IPHWVQT RPEPYPL QWEDHC* GQRVVIS TPQAYPL THSPYPM AHLSIIF QSSKFLH MSMSQWR QAALNRM FRPPMMD IGVHHWN WGLHTFS LPGFSVS KCCATHL DQPMHWI MSHMDSP YILRAGD CMQAHAM DHLMHQR QWEDHWE MQTRLMA HTTKPSL TVTPYPM ACLMCLT HPFNQLQ WTIVGPI KMEPYPL MHSPYPL QLDPYPL HLTAVLT MDILAFH KPNIAIL QSYLYGW QLEPYPL AYPLYPT EQPVHWS GVQKASN MTFTIAA DSFRVWP HLLNGKP AHDPYPL DQPSHWM NAHTYGI HGLHQFN VMRPVTA YPEKSPP LTHATMR APHRLTQ NMSPYPM GYSLHHR ELKIIQP FRLPMTD MFTRGQE RPPMNDL *IDPYPL ITSSHVW LRLPMHD HFGPRQY GPLMPPY VGTIPPL THVSWHI TSSVSLM DRSAFSS GYCAEDT VLGPYPL FRLPMND TCSAKWC GPTRYPL HLGPEQP ERDMRGI KDWPGRL QVTLSGH THWTRAD NKVIYYP LKTMPMI HLQWST* YGGFPMT VRSPFPM RPLPYPL HSLPHER YLSPYPL KATYIVL DRNMHIP ITKPEPN GYGKDWK MSYQIKR YTLSLSL NLEPYPL GMQPKPV WPGPYPL EHPSWAL ALTPYPL AHVPFHH DQTGGTN YARMLLS KMTPYPL TDWLVWE EAVPLRS TGSPSMA KQMPLYH SCPMCYW QEPYPLN KTSADMM GWSEHWS TDSRHVL DSWQKMR RPPMFDS HYKASLM HMTPYPM RIPMFDN MLMPYPL NQAKWVH SIPQASF WGLHTFR SLLMPLQ LSPTYLE RVPMNDG MTDSRAL WGVHQWR KFDGPMQ HLNANSK VYRSVEH HPFNRTS SF*TDHL VGMHTWN GVAHMTT DQPMHWL QWADHW* YGSAFTM SPMPYPL EHFSLHP THSPYPM MRLPMHD NHGPYPL SLMPYPL GQPVHWF HPVPYPM FPP*KHV VLTPYPF VVSASWL SVSETGG QDPYPFS WRLPMSD ELGSALL TIGRYPV KLSPYPL GDQGPNP DQPRHWM MPDPYPL VLGWNMN LQPHHCY ASIFTTW SWSNVSR SSNLTDR QSKYPLD LPSLQVL LDRQALM NHMLSAK NQPYHWL YGLHTFH SADHRML WRTHTWA DQPWHWI HYSYRSI NTFAFNH QYPYPLD QWEYHW* MTPLDAR NYWTQHR TNATTEL TRAPMHD HPFNRSH QWEDHCY QLTPYPL LHPHHWY YTFPFAS QWRSHWN WPLPYPM YEGGSMG RVPMVDG DPTPYPL NAKTSHM TDGQLRR SLEPYPI 254 Phage display (competitive panning) 2 PMID:23826227 Anti-PAP sera sample 3, PAP3 Prostatic acid phosphatase, PAP Not determined. Not determined. Ph.D.-7 phage display library (X7) The phage library was mixed with mouse or human serum. After incubation and washing, protein G was added to the phage-antibody mixture. 2210 VFIPYGH KVMQLHI QMQWAVT YRGISVN AYLFNPM HAPIGNN RDLATLQ TVNNRMY GVWMLPK LVSSYNF TSIMFRY SVSTNSM ETRT*TL QPGRIVQ GIGRVAL LVALRVG ASGSLCC VVPCAGL TYTNPGF ETGSNTI VMDLRIL ISLRIEM MPFWLAR MPYDRSR RIHVDLF TSIGVFS WNSVSRL FFWMTRI VSPVRQL MYQINVT SGEQYRI VPMQTRL SHTNPKS NILANDR ISTHYYL WTVVQTL H*EISVL YSYVHTV HYSATAL QPSYWSR YVQGSRL WPSFVPK TFAGYRV SISHRMY HVRANVP WNLGGGS ASKLSTG YQPVTYR QPDSNVA VIHGNPP SF*TDHL QQHHVHL VSPRAGM DRNPMSF DPAT*TV GSPQVKK RVQDLPH SFRLGSH TVHAAYM WGQLGIR HISKGMT AVQFL*T GVNIRGI GQRVVIS MVHDMLG GNGPLHW VGKMHAN LAAKSGT GTSPDVW HTLVTAR VSWSSSS ITWSEHP AYFNLLR ASHAVGV TEWLRFD SLKHPMY MSWKLLQ LNIKLTL QTGLSSS AFHVTRM AYPKADS VGTTVVK TGSLFLT IARFSAN HTPVLSN VSYGGRT SIYSWAV FRFSCRW NGLVYRE RYLDVWA EMYFTKQ ISLTEHP AQMPVNV YPHPRWT FSSLGSK WSTLTPP SAPDDSF TWIRSAL MLQTNYD DRHFNYL QLSNCCQ GSTSPIV LERMPNP MWRIDFR SQSTVLQ HNPGTHK SGLDLWT QTSGSKL QSQIPNS SLPLVYS HSWIMHT WCRGLVW TFTSYLT AIMHHMV ASLDRYN MIKYRVA SMLIPHP SLYP*IG VMQGVLF NHVS*VS TPHWYVK TEQYTLN QMRPQHT KLYQREP GSNSARS NATMSAS IYPKVGH STYKSML MSRGAFI IWFTFQD APGGVVR ERVLPRV VPLWYVK ATRPLNH EQQTIRL TWS*AQT QLWYKIN FPGVQTQ VLFPDIT TNKTFQL ALFSNGR EASTKLT AIRAPWS IGLSEHF SVPYKLL AAPHPAT YRITIPM YVELIFV TPHWYVR NTSTYLM TVLSAAL TWPEFTK YGFTSNR ELQIVPS LT*KAQY AKVDPED YPHPRWN NFDT*AL VSLTYHI GVAHMTT NAPWWGS SLTEKNW VANTHHG ECRPCAD NIVSNSS DPLLNGV YMTGSKP *TWRGAV VLYSEHP KIPLTSK SVFSFRL SSMRQMH ALVFESY WPTFIPR LDPHRWM TYTGFVS EFAISST WKSGLTY GLIVSPM NTTTLES FQRSTAH SHQDNLL HQRPFVP FMILPGN YEGSIPH WRHVP*T LKLLDSY QLAYSTR GVHYTSA IHMSPPL HVFPCCH TYPERSA IPRWYVK *TSHKYL LARITAE VFAKANP HVDGSLR YRFVNFQ QWRKTES MAFTNAM SFYLPIR LTCLHCN EHPTTQI MGAMARV AVMDKRN DLFARSH TLGTPWS SQVAMIR GSSNLNL EPLSIFS YYPPAHR NAFKMVR YQRPFNP VSQMRSP GQMPFRP SPPLGSS IVDTTWH TVVMYPG SPDPYLR QVYDRSK QGLSRPH SLFPMSL DTPLPPW WMTDRVV KPPMSKY SQGMNLR SHPWTSG WTTTWMY MFSTATA WMTPRWL LPTVRNS TPKWFVK QHYDRSR HWYDRDR HFFTLTI THLLIYM YPWSEQR ICWACSP TGSSWAP HLQTSYT GKLTNVR VGFYEHP RIPIH*N VLYTKNL DGGYWHV AISLTPR SLPT*GS RAPVNSV QEYNRER YPNTDMT LDNALDR DGKQVSI YVRLAVT NTQHVTS 254 Phage display (competitive panning) 2 PMID:23826227 Anti-PAP sera sample 4, PAP4 Prostatic acid phosphatase, PAP Not determined. Not determined. Ph.D.-7 phage display library (X7) The phage library was mixed with mouse or human serum. After incubation and washing, protein G was added to the phage-antibody mixture. 2211 QNIYAGVPMISF EATNSHGSRTMG TVSWSTTGRIPL QLEFYTQLAHLI SMDPFLFQLLQL 5 Phage display (common panning) 4 PMID:23776619 BT483 breast cancer cells Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The surface binding activity of each individual phage clone was analyzed by flow cytometry. The five phage clones exhibited prominent binding to BT483 cells, with phage harboring SMDPFLFQLLQ (PC2) displaying the best reactivity. 2212 AAPSHEHRHSRQ(20) ALAHNPKTTHHR(15) ERPLHIHYHKGQ(8) TTHSKHHFPSSA(5) 4 Phage display (common panning) 3 PMID:23398942 Siliceous spicules Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA The binding specificity/strength of the phages was assessed by the ELISA technique. From the ELISAs it is apparent that the clones HRH-1 (AAPSHEHRHSRQ) and HRH-2 (ALAHNPKTTHHR) bind better than HRH-3 (ERPLHIHYHKGQ) and HRH-4 (TTHSKHHFPSSA) (based on the optical density, data not shown) and those again better than the wild type M13 control phage. The binding specificity/strength of the phages was assessed by the ELISA technique. From the ELISAs, clones harboring AAPSHEHRHSRQ and ALAHNPKTTHHR bind better than those containing ERPLHIHYHKGQ and TTHSKHHFPSSA. 2213 MQGHELGFMKI(1) AETVESCLAKSH(6) MHQGPTSYNLQM(1) HPNGHILLELRQ(1) LSNTLQTTNKK(1) ELQMRALQRLQP(1) CLRNASPHLGCL(1) HTSDGLQLSNVQ(1) WPQGPDRAVLG(1) SVHLYSQMPTKK(1) SRMPTMIMEKGW(1) HPLLTYSASQKG(1) 12 Phage display (common panning) 3 PMID:23551658 Gamma-irradiated Salmonella spp. Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2214 SEAYKHRQMHMS(1) YYPHPPWSPQH(1) HIRWDVNHNSMS(3) VPWVTTYEPWGM(1) VSAARADFYAAM(1) SSYYPQLTAHRF(1) SAKTHPWSIWAY(1) WHNAWESWHYAN(1) KLPPSFDLTGAN(1) GPADNTSKHVIR(1) NRPDSAQFWLHH(1) SWMPHPRWSPQH(1) 12 Phage display (common panning) 4 PMID:23551658 Gamma-irradiated Salmonella spp. Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2215 ALDDYKAGDRGI[3.276 ± 0.060] RQGSWDYKGADR[4.005 ± 0.111] AMAGPDYKVADK[3.858 ± 0.111] QDYKHLDDLLYA[3.036 ± 0.703] YRTMSDYKLDDE[3.688 ± 0.454] D-Y-K-x(2)-D 5 Phage display (common panning) 3 PMID:23807489 Anti-FLAG M2 monoclonal antibody FLAG sequence Not determined. Not determined. SUT12 M13 phage display library ELISA Phage clones from the third round of biopanning were confirmed for their binding to anti-FLAG M2 monoclonal antibody by Phage ELISA. Average OD values at 405 nm were determined. Data were reproduced from the graph and shown as means ± SDs. 2216 NWYLPWLGTNDW(24)[++++] QWELPWLMQPPL(2)[++++] SPGLSLVSHMQT(2)[+++] QLPRTAL(1)[+++] GETRAPL(1)[+++] 5 Phage display (subtractive panning) 4 PMID:23671272 Monocyte-derived immature DCs (iDCs) Not determined. Not determined. Not determined. Ph.D.-12 and Ph.D.-7 phage library pool Flow Cytometry Binding of the phage clones to iDCs was analyzed by flow cytometry. +++, binding <80%; ++++, binding >80-100%. Results demonstrated that all phage clones, but not the control phage, showed strong binding. Prior to biopanning on iDC, the libraries were preabsorbed on human monocytes. A single peptide, NWYLPWLGTNDW (NW peptide), dominated the sequences. The NW peptide shares the motif NW-LPWL with peptide QWELPWLMQPPL. 2217 LDYRSWSPYATS(3) AGELSPNRSAFL(1) QIPPRPPLLTTL(1) HNVTWAALMANV(1) SYNTLTQIAKIR(1) NSTNPHESRPTS(1) Y-R-S-W 6 Phage display (common panning) 3 PMID:23554202 Norwalk virus virus-like particles, NV VLPs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2218 LDYRSWAPYATS(5) IQYRSWIPFSYP(2) FRSYESPNFRPP(2) YRSFDPWYPPVH(1) LTQQRSWSPYMP(1) THQNRQTADIPS(1) Y-R-S-W 6 Phage display (common panning) 4 PMID:23554202 Norwalk virus virus-like particles, NV VLPs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2219 LDYRSWAPYATS(4) IQYRSWIPFSYP(1) LSIRSYTSPQWQ(2) YRSFDPWYPPVH(2) Y-R-S-W 4 Phage display (common panning) 5 PMID:23554202 Norwalk virus virus-like particles, NV VLPs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2220 LPSWYLAYQKII(9) ISWADWTQRWRW(2) ALPTFGVISPFS(1) S-W 3 Phage display (common panning) 3 PMID:23554202 Norwalk virus virus-like particles, NV VLPs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2221 LPSWYLAYQKII(10) SHVSKLVYQSQS(1) S-W 2 Phage display (common panning) 4 PMID:23554202 Norwalk virus virus-like particles, NV VLPs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2222 LPSWYLAYQKII(11) LPSWYLAYQKII 1 Phage display (common panning) 5 PMID:23554202 Norwalk virus virus-like particles, NV VLPs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2223 WSSGMTPDTGAP APWHLSSQYSRT 2 Phage display (subtractive panning, competitive panning) 1/2/3 PMID:23315991 Appetite-regulating hormone Growth hormone secretagogue receptor type 1 Not determined. Not determined. Ph.D.-12 phage display library (X12) With the exception of the first selection round, a subtractive step was performed to remove any background-bound phages. For the subtractive selection step, amplified eluate from previous round of biopanning was suspended in PBST and added to beads that were not coupled with ghrelin. B-ghrelin (biotinylated human acyl ghrelin, Phoenix Pharmaceuticals, CA, USA) were coupled to streptavidin-coated magnetic beads. The bound phages were eluted with either 10 mM DTT (Sigma, St. Louis, MO, USA), acidic elution buffer, or with polyclonal antibody C-18 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) specific for ghrelin. A search in the mimotope database MimoDB 2.0 revealed that peptides APWHLSSQYSRT and WSSGMTPDTGAP have already been selected by other research groups using various targets. The same 2 peptides were also found in different articles and patents using Google search engine. They were considered false positive results. 2224 HHALRLE SHTPTKF ADTVPRH MEMKKTHPVLGA APWHLSSQYSRT 5 Phage display (subtractive panning) 1/2/3 PMID:23315991 Appetite-regulating hormone Growth hormone secretagogue receptor type 1 Not determined. Not determined. Ph.D.-12 and Ph.D.-7 phage library pool With the exception of the first selection round, a subtractive step was performed to remove any background-bound phages. For the subtractive selection step, amplified eluate from previous round of biopanning was suspended in PBST and added to beads that were not coupled with ghrelin. B-ghrelin (biotinylated human acyl ghrelin, Phoenix Pharmaceuticals, CA, USA) were coupled to streptavidin-coated magnetic beads. The bound phages were eluted with either 10 mM DTT (Sigma, St. Louis, MO, USA), acidic elution buffer, or with polyclonal antibody C-18 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) specific for ghrelin. A search in the mimotope database MimoDB 2.0 revealed that the peptide APWHLSSQYSRT has already been selected by other research groups using various targets. The same peptide was also found in different articles and patents using Google search engine. It was considered a false positive result. 2225 ANVQFML SINDLTF EQFLLIG QSYLWHM 4 Phage display (common panning) 1 PMID:23315991 Appetite-regulating hormone Growth hormone secretagogue receptor type 1 Not determined. Not determined. Ph.D.-7 phage display library (X7)