2176 NHQQQNPHQPPM 1 Phage display (common panning) 4 PMID:23350661 U251 glioma cells Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2177 YEVCGSDTVGC 1 Phage display (common panning) 3 PMID:23278311 Serine/threonine-protein kinase StkP Not determined. Not determined. Not determined. ANL4 phage display library (X10C) 2178 YTFGLKTSFNVQ 1 Phage display (subtractive panning) 3 PMID:23521800 Prostate adenocarcinoma cells, PC-3 cells Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Negative selection: A 10 ml solution amplified from the positive selection was added to a T25 culture flask and incubated with CO2 environment. Rounds of positive and negative selection were performed three times. 2179 PRSLHRP AKTPPSR GKPNQGR RRPADRP QSSNFDC KPAAHAV PGASVEW STAQSLK RISGLRV QLTLDAA NAPRKTP LRKESHA IRAGRGV EAKAILT RNQPRAL HQRSQTK 16 Phage display (common panning) 2 PMID:23503763 Recombinant serotype D NTNHA, rNTNHA-D Botulinum neurotoxin type A, BoNT/A Not determined. Not determined. X7 T7 phage display library The random heptapeptide-displaying phages that display the affinity to the NTNHA-D protein were screened by using the QCM-assisted method. 2180 KGHSLMP IPTLPSS 2 Phage display (common panning) 4 PMID:23313229 Anti-human KGF mAb Keratinocyte growth factor, KGF Not determined. Not determined. Ph.D.-7 phage display library (X7) 2181 LEPKGRYDDPWT ATRYDDIWASTA R-Y-D-D-W-T 2 Phage display (subtractive panning) 3 PMID:23499997 Anti-rabies virus IgG Glycoprotein G Not determined. Not determined. Ph.D.-12 phage display library (X12) Before screening, phages were incubated with non-immunized dog IgG to exclude non-specific adsorption of the anti-rabies virus IgG. Then the incubated phages were allowed to react with the positive IgG. 2182 DYPPYLDRYAHW TATTDIRIPYTE YFDNTAKNAPLL GSHSRIGSVSMY TPLHRYFHWDSP TSKALYTHYQFL HYQFSDVRHAWK LPKNNLGLDALL IDTFYMSTMSHS FMGPQESTLQRL MNQTTKFSIHGA DNDEPGSRYNWW SDKMWHPMYAAP SMTQASDIGRLW KQLHPIYVQNGT APWHLSSQYSRT TWKYDGHRFYQA AKTLYPLYSSPI ARYDAIWNSEAL AGTGDDIARMWR TDVFLPRYDRLW NVLFFRIMMRHW SKNLFTGYQSSS SKYASQNYFTPD SYIDRYHWWSTQ VIVPDRHSHWLT GNVDVRWIWGNF DNHEHRFWHWDS YFDNTAKLAPLL TEQSVSRYDHLW 30 Phage display (subtractive panning) 3 PMID:23499997 Anti-rabies virus IgG Glycoprotein G Not determined. Not determined. Ph.D.-12 phage display library (X12) Before screening, phages were incubated with non-immunized dog IgG to exclude non-specific adsorption of the anti-rabies virus IgG. Then the incubated phages were allowed to react with the positive IgG. 2183 YSDEDLARVWRR KMSWDIGRVWNI S-D-R-V-W 2 Phage display (subtractive panning) 3 PMID:23499997 Anti-rabies virus IgG Glycoprotein G Not determined. Not determined. Ph.D.-12 phage display library (X12) Before screening, phages were incubated with non-immunized dog IgG to exclude non-specific adsorption of the anti-rabies virus IgG. Then the incubated phages were allowed to react with the positive IgG. 2184 FETLPSR NPLLSIQ LQGAHLR IPPLYFS QWPLMTT WRPPMLV HSQAAVP 7 Phage display (common panning) 3 PMID:23466786 Fibroblast growth factor 8, FGF-8 Fibroblast growth factor receptor 3, FGFR-3 Not determined. Not determined. Ph.D.-7 phage display library (X7) 2185 CTSQLLTHC CQHSKLSTC CIDLLHHKC CTGSLAISC CLGPAPHSC CQPAQSWSC CTPHRLAAC CRLHHTLSC CDPLKPSAC CHNPRANLC CTPHRLAAC CTQNPSSSC 12 Phage display (common panning) 4 PMID:23352850 NEP1–35 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2186 RTGNIVTAWK*X RRQTLSHQMRRP RRQSSRRMMRRI TNRKKRMISRMS MNRIPLPSITRR QHRLKPIMTRRQ RMRLNRRPKRMT MXLXRTRXXPKM RMLRMSRSSHRK RPLPRSKRNISR RRRKLRTQRLII KSHRRKRIRLLS SKRRNLQSRRKN TQRSKHRRRKNR HHRTRLRSMSRQ NSIHLMRLMRSI KSMQRRIKIINR R-R-x(7)-R-R-x 17 Phage display (common panning) 4 PMID:23352850 NEP1–35 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The displayed peptide with strongest affinity was synthesized as PepIV (RRQTLSHQMRRP) and its effect on axonal regrowth was tested in neurite outgrowth assay. PepIV could neutralize myelin inhibition and promote neurite regrowth efficiently. It was indicated that application of small peptides to block targeted myelin inhibitors may improve CNS regeneration. 2187 SPSIDTRYSRLG(3) CVSVGMKPSPRP(3) SVSVGMKPSPRP(3) MVSMDSSPRDRL(1) x(2)-S-x(7)-R-x 4 Phage display (common panning) 4 PMID:23338703 The human colon carcinoma Caco-2 cell Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) After four rounds of selection, 30 individual phage clones were randomly selected and individually amplified. Of the 30 phage clones, 10 exhibited relatively high binding capabilities to Caco-2 cells. The novel SP-2 (CVSVGMKPSPRP) peptide, which could specifically bind to CRC cells, was selected in this study. This peptide has much potential to be developed as a more effective probe or marker for the early diagnosis of CRC. 2188 HLNILSTLWKYR(6) KLLTLSNWPNIR(4) HLPTSSLFDTTH(2) HLRLQDALPTRP(2) HLSWRDLQVQSY(1) HLPQNFTKLSP(1) HLPQNFTKLS(1) HLPQNFTKL(1) GSSHRLSWSQ(1) GHSQFASPSTAL(1) WH*GKISPLQVF(1) 11 Phage display (common panning) 3 PMID:23296614 Protein S100-B Calcium Not determined. Not determined. Ph.D.-12 phage display library (X12) 2189 MTVATPV NDVFRSG SISLEPL FYNVTVP FYNVTVP TLQFHTQ MHIAHSK MHIAHSK THWWRLW 9 Phage display (common panning) 4 PMID:23054431 BetaC1 Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2190 VLLAVWSVFHERSWL CLIVCLFIVRCGGCF GCNVWMCPDFCLPAR LGVVGFFPFDPSVLL SLYLPCVLPDLRFRM CGFAFYYVWLMVVMW QFFFSDDGSPPFEVG CSQGLLHSAVFALCF MLVCIFFFGMLLIMF FCVCFCGCTIMVLLL SSCNLVLAAHQFCGD LSLGSGFVVSYGVSG ASPLIVAAACSSYFL AFVSGADVNVCLHCA LFISIFVLAFCFALV SCLAVVLNCSGGLWS FCVSNGTTCVCIGVV TDVDFLFPNIHVSFG SVFCVSGSSSHWLIE FCVNNGTTCSVWRCD RVFFVIFLSDLIWPF SNAPCIVDMSSPLVH YSCDAYVGVRGVLCI GCVFILWGISSSHDA IFDALSLVCLSVQPS SASCFTGGMVRAHVR LHLIGLAASSGLSDL CVPSFGFVLPNRLAA LCCSVNLELSPSMGG VRPHPGLARSLPVAR ICNSVSLFVFGPYGA CDFLCVRSAVGTMVA CVYPAFTPFGQYTLL FLPGIGYASVLCCSG 34 Phage display (common panning) 1 PMID:23514038 Brz2001 DWARF4 Not determined. Not determined. X15 T7 phage display library Panning was performed using the QCM biosensor-based T7 phage display. The QCM sensor was immersed into the cuvette containing buffer and then allowed to fully stabilize. A T7 phage library was injected into the cuvette. Frequency changes, caused by binding to the Brz2001 immobilized on the gold electrode surface, were then monitored. 2191 TLHPAAD(7) NERALTL(5) SHSGYFS(5) APTEHWA(2) HATRPPM(1) TFNFPPV(1) AETHINQ(1) TMHGYDT(1) 8 Phage display (common panning) 3 PMID:23623136 Epoxy Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) The results show that phage displayed peptide sequences exhibit high hydrophobicity, with low ratios of hydrophilic to total number of residues. The fluorescent characterization for the binding of TLHPAAD to a flat epoxy surface was investigated. Significantly, the surface with peptide-displaying phages shows much higher fluorescent intensity relative to the one incubated with M13 phages (without phage displayed peptides on the coat). Further, we investigated the localized binding of phage displayed peptide (TLHPAAD) to epoxy microstructures. The epoxy microstructures on glass were generated by using stencil masks to cover epoxy, followed by plasma oxidation. The phage displayed peptides were then allowed to bind to these epoxy microstructures, and the bindings were characterized with fluorescent characterization. Significantly, stronger fluorescent signals were observed localized on the epoxy patterns, relative to the glass substrate. This result clearly indicates that the microstructured epoxy in parts enhanced binding affinity toward phage displayed peptides over the host glass substrate. 2192 RDLHSNDVV(2) DKSSNDSIA(2) SNDGVWAIP(1) LFDRESNDW(1) YTDSNERTT(1) YRDSNEHQP(5) SND, SNE 6 Phage display (subtractive panning) 3 PMID:1720463 Anti-IL1β(163-171) monoclonal antibody Interleukin 1β, IL1β Not determined. Not determined. X9 PC89-based phage display library The library was incubated with the mAb and the mix was transferred to a streptavidin-coated petri plate. The 2nd round of biopanning was preceded by a pre-absorption step of the panned library on anti-mouse IgC antibody. Then the library was biopanned against anti-IL1β(163-171) mAb. 2193 YPVDRSTFW LASAGGQDN YPVDRSTFW NPIDHSARS NPIDATARS GPPVDPTSG DQWTAVGSK 7 Phage display (subtractive panning) PMID:11179365 Anti-TcTS Abs Trans-sialidase, TcTS Not determined. Not determined. X9 PC89-based phage display library The library was diluted to 100μl in phosphate buffered saline (PBS) and preadsorbed for 2 h with protein A-Sepharose 4B. Supernatants from four washings with 100μl of PBS were pooled, and 5 μg of\r\npurified antibodies was added. 2194 CPRVRSKPVVC CRRRLRGTWVC CSTDPAATDC CGPPVAPTSAC CVPLPLLRSKC CPRVRSKPVVC CPLSLRSKC CPRVRSKPVVC 8 Phage display (subtractive panning) PMID:11179365 Anti-TcTS Abs Trans-sialidase, TcTS Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C) The library was diluted to 100μl in phosphate buffered saline (PBS) and preadsorbed for 2 h with protein A-Sepharose 4B. Supernatants from four washings with 100μl of PBS were pooled, and 5 μg of purified antibodies was added. 2195 HPLKQYWWRPSI(22) PIWWKHSGGPIL(1) YWWRDAPVSQGR(1) SYPTDKWWIKPG(1) W-W 4 Phage display (common panning) 3 PMID:11849705 E. acerulina sporozoites Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) PW2 (HPLKQYWWRPSI) disrupts the sporozoite pellicle, resembling the effect caused by most natural antimicrobial peptides. PW2 peptide was also effective against fungi and showed low activity against Toxoplasma gondii tachyzoites, but no activity against Trypanosoma cruzi, Crithidia fasciculata epimastigotes, and bacteria. Additionally, the parasiticidal concentrations of PW2 produced a very low lytic effect on mammalian and avian cells. The effectiveness against Eimeria sporozoites and the absence of adverse effects to host cells indicates that PW2 may be used as a model to generate new drugs for the control of avian coccidiosis. 2196 VQWWRPT(7) NWWRPLP(1) GKWWVFD(1) VPTKPWW(1) W-W 4 Phage display (common panning) 3 PMID:11849705 E. acerulina sporozoites Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) 2197 CPWWKTSKC(6) CPWWKASSC(1) CTPTWWRTC(1) CAPTWWKSC(1) CWWTSASRC(1) CSARWWQPC(1) W-W 6 Phage display (common panning) 3 PMID:11849705 E. acerulina sporozoites Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2198 SAGPYIDMMSIW KLQGNPSGPGYW ITGDRLAYFAYR DYLSNPKGPSHR DRAHEQEWFYKT TYRIGPYQGYHH FLPGIGDHRHSD GMRAGPMQNWTA FGPGSADFRHMN FSPGSADQKPHH GLRIGTGQSYWL 11 Phage display (subtractive panning) 3 PMID:23800339 Polyclonal RRi-11 plasma Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Three rounds of alternating positive/negative selection were performed. Positive selection used week 7 plasma from a protected animal, RRi-11. Positively selected recombinant phages were counter-selected with plasma from the same vaccinee but collected at week 0 (containing vaccine-induced Abs only). 2199 ESRQLYPGFQAF VIIGPPYQHWSM TLLRIPPGGVFM 3 Phage display (subtractive panning) 3 PMID:23800339 Polyclonal RTr-11 plasma Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Three rounds of alternating positive/negative selection were performed. Positive selection used week 7 plasma from a protected animal, RTr-11. Positively selected recombinant phages were counter-selected with plasma from the same vaccinee but collected at week 0 (containing vaccine-induced Abs only). 2200 TPFDLRPSSDTR(26) TDIRLRPLDKRI(4) RLKPRSDIKPIR(1) SDTPLIKRLDFP(1) RRSDLKPIRPLK(1) RRKIKPRRRIRP(1) TRIRPLRRRKPI(1) RRIPKLIKRRLR(1) 8 Phage display (subtractive panning) 3 PMID:23675691 Glyphosate Glyphosate receptors Not determined. Not determined. Ph.D.-12 phage display library (X12) Glyphosate-decorated glass beads were employed as binding targets and APES-coated glass beads as the negative screens during the phage library screening process. Phage colony containing TPFDLRPSSDTR(P1) has the highest binding ratio (19.2%). The second highest is TDIRLRPLDKRI(P2), which has a binding ratio of 5.2%. Both P1 and P2 contain a homology tripeptide sequence DXR (X = L,T, I, or K).