2151 NSLTPCLGRVLANC(3) NSLTPCGRTRVTSC(2) NSLTPCPYPRKGSC(2) NSLTPCRFLRRTVC(1) NSLTPCVPKKNRTC(1) NSLTPCRGRTHPLC(1) NSLTPCVPKKNRTC(1) NSLTPCNICARQYC(1) NSLTPCVRNSLTPC(1) NSLTPCRGRTLHLC(1) NSLTPCRGRTHPRC(1) SLTPCSYVGKGSC(1) SLTPCDTKKNHCC(1) NSLTPCGRTRDN(1) 14 Phage display (common panning) 4 PMID:18564683 Anti-protein monoclonal antibody BPM Not determined. Not determined. Not determined. NSLTPCX7C T7 phage display library 2152 NSLTPCGRTRVTSC(2) NSLTPCAREVTLLC(2) NSLTPCGRTRDN(2) NSLTPCGPKRKATC(1) NSLTPCSGLLVANC(1) NSLTPCSKKNPGNC(1) NSLTPCGAESLTPC(1) NSLTPCGSESLTPC(1) NSLTPCCKSLRPHC(1) NSLTPCAKTRTAKC(1) NSLTPCRTKKSGTC(1) NSLTPCFTVARACC(1) NSLTPCKTRKSGSC(1) NSLTPCFTVARACC(1) NSLTPCGAESLTPC(1) 15 Phage display (common panning) 4 PMID:18564683 Anti-protein monoclonal antibody BPA Not determined. Not determined. Not determined. NSLTPCX7C T7 phage display library ELISA Peptide-displaying phage types were designated according to the selection procedure with each type of phage and antibody; T7/9D5, T7/4B11, T7/BPA, T7/BPM, and M13/4B11. Bound phage was considered specific if the absorbance at 492 nm was at least 0.05. The selected bound phages, T7/BPA were ELISA-positive for B. pseudomallei MAbs. 2153 SHSSSNSEQLNFVMKVVSRP(4) SHSSSGYVGPRLGSGIGSRP(1) SHSSSTVVMGRVWQYEQSRP(1) SHSSSGYVGPRLEVGDWV(1) 4 Phage display (common panning) 4 PMID:18564683 Anti-EPS monoclonal antibody 4B11 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA Bound phage was considered specific if the absorbance at 492 nm was at least 0.05. Using a random 12 peptide M13 phage library, 7 from 10 selected bound phages (70%) gave positive ELISA results. 2154 PMSYRY TSSYRL VESYRV SYR 3 Phage display (common panning) 2 PMID:19377945 Anti-uPAR monoclonal antibody IIIF10 Human urokinase receptor, uPAR Not determined. Not determined. f3-6mer phage display library (X6) 2155 TARGLSARTAFAGLV PISGRHLSSSFRFGG GEPSVVISRSSRLSP 3 Phage display (common panning) 2 PMID:19377945 Anti-uPAR monoclonal antibody IIIF10 Human urokinase receptor, uPAR Not determined. Not determined. f3-15mer phage display library (X15) 2156 DLLWLL DLLWEL DLLWII DLLFEL DLLYLL DLLTIL DLLYDL DLLWSL DLVWLY WDFPAY WEFPAY PDWHEM PSWEDD EPPWVW GPWMLW D-L-L-[WY]-x-L, W-[DE]-F-P-A-Y, P-x(2)-W-x(3), x-P-x(3)-W 15 Phage display (common panning) 2 PMID:19377945 Anti-huPENK monoclonal antibody Not determined. Not determined. Not determined. f3-6mer phage display library (X6) The phage-antibody reaction suspension was transfer to the streptavidin plate. 2157 YPLQPILAFPLA TLQSILPPRLWS VMHPSHFPVDAL WAITKPAPAAHP TPNLLVHMGVKL FMGHHMVEPWDV GFSGHRLFDLSS NPPLYPAAQTYE 8 Phage display (common panning) 3 PMID:20423858 Aminopeptidase N Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA,Fluorescence Ten selected phages were verified by ELISA, and the absorbance at 450 nm was determined. Only one clone had an higheELISA OD value. Synthesized peptide P7 (GFSGHRLFDLSS) bound to the cell membrane of THP-1 cells as shown by immunofluorescence assay. The binding of the peptide P7 to THP-1 cells was blocked by CD13-specific monoclonal antibody WM15 at different levels. 2158 MWPTTTHSSPYH(9) FDWKYNLLAHPQ(1) 2 Phage display (common panning) 4 PMID:20423858 Aminopeptidase N Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA,Fluorescence Ten selelcted phages were verified by ELISA, and the absorbance at 450 nm was determined. Nine phage clones had higher OD values. Synthesized peptide P9 (MWPTTTHSSPYH) bound to the cell membrane of THP-1 cells as shown by immunofluorescence assay. The binding of the peptide P9 to THP-1 cells was blocked by CD13-specific monoclonal antibody WM15 at different levels. 2159 CRTIGPSVC CRDGISVIC CWKHSGLGC CYGGGGRSC CYLGRSRWC CWGRSDGAC CDRSAGDSC CGGARGFLC CKVPSRSMC CGSAWGFLC CRVGPRMTC CRPRGDALC 12 Phage display (in vivo) 3 PMID:21183793 CNS endothelial cells Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The phage library i.v. was administered into normal mice and recovered the enriched population of brain-homing phage after 3 rounds of selection 2160 QFPPKLTNNSML(18) SYDILKPNPQRL(5) SHGKPPSFSPYT(4) 3 Phage display (subtractive panning, in vivo) 6 PMID:21258297 Lewis lung carcinoma (LLC) tumor cells Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) A random 12-mer peptide library was pre-selected in vitro on HUVEC (one round of negative selection) and on HBOEC (three rounds of positive selection) which resulted in an enriched phage pool, designated as mini-library. The mini-library was used for in vivo panning of tumor-associated phage (three rounds of in vivo panning). 2161 ASSGMCFTKKTVLC[1.334] 3 Phage display (subtractive panning) 3 PMID:21339361 Human factor H Not determined. Not determined. Not determined. ANL4 phage display library (X10C) ELISA Each clone was tested for binding to factor H by ELISA. The absorbance at 405 nm was determined, reproduced from the graph and shown. The absorbance of phage CK, unrelated control phage from library ANL4, was 0.094. The libraries were prescreened by incubating the library in BSA-coated wells for 1 h at room temperature; supernatants were then transferred to factor H-coated wells for binding. 2162 ASSYDVGYSHDCRF[1.235] ASSSRCTYDHWCSH[1.395] ASPSWCSYSHWCRH[1.332] ASSFKCDYSHWCLH[1.311] ASSNVCSYSYWCAH[1.102] ASSCMYSYWCTH[1.232] A-S-S-x(2)-C-X-Y-S-H-W-C-x-H 6 Phage display (common panning) 3 PMID:21339361 Human factor H Not determined. Not determined. Not determined. ANL5 phage display library (X8CX2) ELISA Each clone was tested for binding to factor H by ELISA. The absorbance at 405 nm was determined, reproduced from the graph and shown. The absorbance of phage CK, unrelated control phage from library ANL4, was 0.094. 2163 CKRGNSGSC CQRLVGFAC CLQASPNFC CNGSVRSFC CPGFGLAYC CFLFTFEAC CRGVLMRYC CRAFVVASC CQSHSAFVC 9 Phage display (subtractive panning, in vivo) 4 PMID:21829599 Human CD4+CD25+ thymic cells Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) CD4+CD252 cells were used for preclearing and CD4+CD25+ thymocytes were used for biopanning and selection of bound phage. 2164 YPHYSLPGSSTL 1 Phage display (subtractive panning) 3 http://www.zgylxtb.cn/oa/DArticle.aspx?type=view&id=200903007 NCI-H1299 non-small cell lung cancer cell line Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The lung cancer NCI—H1299 cell line was used as the antigen and MRC-5 was used as capture cell for subtraction biopanning from a phage display peptide library. 2165 CSPLSQSAC 1 Phage display (subtractive panning) 4 http://www.cqvip.com/QK/92593A/200712/26118140.html THP-1 cells Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) THP-1 cells stimulated by lipopolysaccharide were used as target cells and untreated THP-1 cells as absorbing cells. 2166 CRAKSKVAC(15) CSRPRRSEC(3) CNRRTKAGC(1) CSRPRRSVC(1) CSRPRRSWC(1) 5 Phage display (in vivo) 3 PMID:14667505 Dysplastic skin Not determined. Not determined. Not determined. CX7C T7 phage display library Two rounds of selection ex vivo and one round in vivo were performed. Forty-eight clones were isolated from the vivo round. 2167 RLDPTSYLRTFW HDSQLEALIKFM 2 Phage display (common panning) 3 PMID:23440926 Primary cultured chondrocytes Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 23 single phage plaques were randomly picked and sequenced. Alignment analysis revealed that the sequences of phage clones RLDPTSYLRTFW and HDSQLEALIKFM exhibited some amino acid sequence similarity, while the remaining sequences showed no clear similarity to each other. 2168 SWTYSYPNQNMD(3) DWTYSLPGLVEE(1) NWTWSMPTGNPA(2) GMTLRVLTNYTE(1) TLHVSENSWTYN(1) DWLWSFAPNVDT(2) TLSSQNPYMHKK(1) IDKQMMTSHKAI(3) QGMETQKLRMLK(1) GWYWETPLDMFN(1) GWVIDYDYYPMR(1) VTAENYQSFSVS(1) NNKMSSEMMSIV(1) STGTDLHSNARI(1) YEFDNLLNRTLW(2) EWTVNERTMWDL(1) 16 Phage display (subtractive panning) 2-3 PMID:23483995 The embryonic progenitor cell line W10 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Cell targeting peptide phages were selected from a 12-mer linear peptide display library by 3 rounds of selection against undifferentiated W10 progenitor cells which included a negative selection against adult human dermal fibroblast (HDF) cells at each round to remove peptides binding common cell surface markers. The specificity of peptide phage cell binding for the displayed peptide was determined by performing competition experiments with synthetic peptides to indirectly measure the ability of the free peptide to bind the surface of W10 cells. It is indicated that cell surface binding of the 4 selected peptides(YEFDNLLNRTLW, GWVIDYDYYPMR, GWYWETPLDMFN and DWLWSFAPNVDT) was sequence specific and independent of display on the phage particle. 2169 EMYRKPMHAQLR(1) SMKPAPPVQHQL(1) EMKPMAPITRYT(1) KPMPPMIRLVTS(1) GIAKKPSAPLQR(1) ASNMKPSAPMQR(1) K-P-x(2)-P-x(2)-R 6 Phage display (common panning) 4 PMID:23613856 Anti-topo-I antibodies, pt4 anti-Ap1-17 Abs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Panning of a phage display peptide library with anti-Ap1-17 antibodies from 2 patients identified novel, partially overlapping motifs KPxxPxR as the result of the alignment of specific phage clone insert sequences. 2170 IPIPRESIKPTW(18) DPFTRMSIKPTG(1) DLSPRLTIKPQR(4) DPMSRITMKPHI(1) LPWIRTTEKPQF(1) DHPQTRTAPKPV(1) DPHYRNSPKPDS(1) NYRETPKPTWPT(1) EFRSSVKPQHPL(2) AHDHRSSLKPTR(4) QGHMRQTAKPFV(1) R-x-[ST]-x-K-P 11 Phage display (common panning) 4 PMID:23613856 Anti-topo-I antibodies, pt4 anti-Ap1-17 Abs Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) Panning of a phage display peptide library with anti-Ap1-17 antibodies from 2 patients identified novel, partially overlapping motifs KPxxPxR as the result of the alignment of specific phage clone insert sequences. 2171 SGHQLLLNKMPN(1) RLLFRKIRRLKR(5) MDMRTTDIRDTS(1) RNHPATLTGTGG(1) GILSELGKALGG(1) GAPALSTPPLSR(1) 6 Phage display (subtractive panning) 6 PMID:23409125 The cell surface of E. coli Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) In order to eliminate non-specific binding phages and to select peptides that bind specifically to E. coli, a subtractive phage-display approach where the library was first pre-adsorbed against S. aureus ATCC 25923 was used to eliminate phages binding to the Gram-positive cell surface. The remaining phage library was then used to affinity select for peptides binding to whole cell surface of E. coli ATCC 700928. 2172 CGSKTQAPC CGLPWLSTC CSTPHNLGC CLATGNQIC CPATLTSLC CHGAPNRLC CRPPIGAFC CTSQSQHMC CGPAVYMKC CQGDLPGYC 10 Phage display (subtractive panning) 3 PMID:23533448 Tumor necrosis factor alpha, TNF-α Anti-TNF-α polyclonal antibody Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) After negative selection on a BSA-coated well, the phage library was then incubated with TNF-α. 2173 CSPHTTIAC CEPFAGRSC CTSPLPGTC CSYEAHQTC CNNPLKSLC 5 Phage display (subtractive panning) 4 PMID:23533448 Tumor necrosis factor alpha, TNF-α Anti-TNF-α polyclonal antibody Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) After negative selection on a BSA-coated well, the phage library was then incubated with TNF-α. 2174 TVKYSTLVEWPY(8) GFRFGALHEYNS(16) NSIALINDTHKR(2) TWKFEPLGTFID(3) DTFHSPLVALVS(5) YTSHFPLETWPQ(5) ETVRQAEELFYV(1) SFRFFPLDMWPH(5) AYTTVPYMATLP(1) GGSIAASELEYY(2) HSTLKLGALTNY(1) GSFHSPLLAYVS(1) DTGHSPEPGKVP(1) GTFHSPLLDHKS(1) TVTFAPLRMWHP(1) GWLRSPSLLFSN(2) TYTFRPLYEPPL(1) GYTFQPLNEWAI(1) 18 Phage display (subtractive panning) 4 PMID:23365656 VPAC1 receptor VIP peptides Not determined. Not determined. Ph.D.-12 phage display library (X12) The phage library was first added to Chinese hamster ovary cells (CHO-K1 cells). After the incubation,the supernatant was collected, and the CHO-K1 cells (and the phages bound to them) were removed by centrifugation. The supernatant containing phages was incubated with the blocked CHO-K1/VPAC1 cells. Among these 18 phage clones, GFRFGALHEYNS appeared to have the highest OD450nm and selectivity value, and it bound more effectively than any of the other clones. 2175 FTHEAFG(6) HLQHYQV(2) KWDATYT(2) VRWDATT(2) IGHGTWG(2) HASPGQG(1) NPHEQAG(1) KVWTLPR(1) RWDATRE(1) W-D-A-T 9 Phage display (competitive panning) 4 PMID:23308124 Anti-CFP10-ESAT-6 (CE) pAbs CFP10-ESAT-6, CE Not determined. Not determined. Ph.D.-7 phage display library (X7) Bound phage was eluted with 100ml solution of the free anti-CE antibodies (100 ????g/ml in TBS) to compete the bound phage away from the immobilized antibodies on the plate.