2126 CPIEDRPMC(8) CPIHDRPMC(3) CAIRDRPMC(1) CALRDRPMC(2) CAPQLRPMC(1) CPEWARPMC(1) CPLDKRPMC(1) CSLERRPMC(1) CELWQRPMC(1) CDLPMHPMC(1) CQPPMFYSC(2) CLPSKFSHC(1) CDHPVPWRC(1) CSLRTAAAC(1) RPM 14 Phage display (subtractive panning) 3 PMID:14670181 Human colon cancer cell line HT29 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The phage pool isolated after one round of selection against HT29 cells was subtracted by five rounds of successive incubation with HCT116 cells. 2127 CPIEDRPMC(6) CPIDERPMC(3) CPIHDRPMC(1) CALRDRPMC(6) CPMHQRPMC(1) CPLASRPMC(1) CPEKFRPMC(1) CVPEQRPMC(1) CDLPMHPMC(1) CQPQSQPMC(1) CQPPMFYSC(3) CFESQSRLC(1) CIHPVPWRC(1) RPM 13 Phage display (subtractive panning) 4 PMID:14670181 Human colon cancer cell line HT29 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The phage pool isolated after one round of selection against HT29 cells was subtracted by five rounds of successive incubation with HCT116 cells. 2128 PPWYSKTDL(2)[1.31 ± 0.02][+++] PPFYLKEVR(1)[0.70 ± 0.06][++] 2 Phage display (subtractive panning) 2 PMID:11169438 Anti-CD18 monoclonal antibody MHM23 Integrin beta-2 Not determined. Not determined. pVIII-9aa phage display library (X9) ELISA,Dot blot In phage ELISA, average values (A405 nm) were obtained after two independent experiments of ELISA assays. The standard deviation of each pair of measures is reported. Data were expressed as means ± standard deviations. The absorbance of the wild type (wt) phage was 0.05 ± 0.00. Besides, positive clones were further tested in dot-blot. Results were expressed as symbols like ++ and +++. Biotinconjugated anti-mouse IgG Fc-specific secondary antibody was pre-adsorbed overnight with M13K07 UV-killed phage particles. Then specific complexes originated by incubating mAb MHM23 with library and secondary antibody at room temperature (RT) were immobilized on streptavidin-coated dishes. 2129 CPPPFYRRGEC(8)[1.04 ± 0.08][+++] CIPNAPIWIKC(5)[0.50 ± 0.01][++] CEPPFFRKLPC(3)[0.61 ± 0.07][++] CRLPPFSRKLC(1)[0.33 ± 0.04][+/-] CNHPIFYARWC(1)[0.24 ± 0.00][+] P-P-F-Y-R-K 5 Phage display (subtractive panning) 2 PMID:11169438 Anti-CD18 monoclonal antibody MHM23 Integrin beta-2 Not determined. Not determined. pVIII-9aa.Cys phage display library (CX9C) ELISA,Dot blot In phage ELISA, average values (A405 nm) were obtained after two independent experiments of ELISA assays. The standard deviation of each pair of measures is reported. Data were expressed as means ± standard deviations. The absorbance of the wild type (wt) phage was 0.05 ± 0.00. Besides, positive clones were further tested in dot-blot. Results were expressed as symbols like +, +/-, ++ and +++. Biotinconjugated anti-mouse IgG Fc-specific secondary antibody was pre-adsorbed overnight with M13K07 UV-killed phage particles. Then specific complexes originated by incubating mAb MHM23 with library and secondary antibody at room temperature (RT) were immobilized on streptavidin-coated dishes. 2130 YCPRYVRRKLENELLVL(2)[0.089] PCLMYSKYNLLYPYPAY(1)[0.092] GCMMYRYPAPGNHHIQM(1)[0.034] RCCTRKRFLRWYGHSLL(1)[0.237] SCHFEALRRQTYMYFYV(1)[0.205] 5 Phage display (subtractive panning) 3 PMID:15182617 The ECs injured by ox-LDL Not determined. Not determined. Not determined. XCX15 phage display library ELISA Identification of positive phage clones was performed by ELISA. Absorbance at 450 nm was determined, reproduced from the graph and shown. If A values of phages are twice as likely to that of the negative control, these phages are positive ones. ELISA results demonstrated that all six phages were positive. Before incubation with the endothelial cells (ECs) injured by oxidized low density lipoprotein (ox-LDL), the library was pre-absorbed with normal Ecs. 2131 CVPELGHEC(73) CELGFELGC(1) CFFLRDWFC(2) 3 Phage display (subtractive panning) 3 PMID:15480432 Tumor-related antibodies Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) The CX7C random peptide library were incubated with antibodies immobilized on protein G from serum. The pre-cleared phage library was then selected on affinity-purifed antibodies from ascites of the index patient. 2132 CHTFEPGVC(35) CAPSQTYHC(2) CPPEAMHLC(2) CKAMSWYAC(2) CTSPTNRSC(2) CHGKYFVSC(2) CNTHMTAFC(2) CPSTLTSSC(1) CPSTLTSSC(1) CKIPSAFAC(1) CSRESPHPC(1) CQSRLSLGC(1) CLDHFAPMC(1) CLDKKTTSC(1) CNMSPQLDC(1) CSQRQTLDC(1) CSTKLLHEC(1) CPHSPTSLC(1) CPQRHVNYC(1) CMMSQLAHC(1) CPMAHLEFC(1) CELIKESRC(1) CQPENLPTC(1) CPFKLSKHC(1) CASSLHTIC(1) CHPLRLPAC(1) CHQSVNKEC(1) CLQNPTPEC(1) CPTEAQLQC(1) CLFAQLGPC(1) CNQPTRALC(1) CTPRTQKAC(1) CIHFPSASC(1) CPLRIAQHC(1) PST, AM, SRS, LD, SPT, HY, SQ, MS, SS, AQL, CHTFEPGVC 34 Phage display (common panning) 3 PMID:15508130 Human MTC-derived TT cells Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2133 CHTFEPGVC(35) CHTFEPGVC 1 Phage display (in vivo) 3 PMID:15508130 Human MTC xenografts Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) Female nude mice carrying pre-established human MTC xenografts following subcutaneous injection of TT cells were anaesthetized with avertin and intravenously injected with input phage library . 2134 FPSKTGAFVPFS NNSQKPAPVSPF QLSPVLARHNIS TKNMLSLPVGPG RHLPTLFAPTPT PRGVWTTMSLPH LPLTSLMPLGLH SVSLPYANLATH RLLDTNRPLLPY LPY 9 Phage display (subtractive panning) 5 PMID:15520208 Nasopharyngeal carcinoma (NPC) cells Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) ELISA,Competition experiment Of 44 phage clones reacted with NPC and NNM cells, 16 NPC-bound phage clones were selected by ELISA assay. When a peptide-competitive inhibition assay was performed to discover whether the synthetic peptide and the selected phage clone competed for the same binding site, the results showed that the binding activity of NPC cells with the L-phage (displaying RLLDTNRPLLPY) was inhibited by synthetic peptide (L-peptide, RLLDTNRPLLPY) in a dose-dependent manner. After being subtracted with NNM cells three times, the unbound phages were used to react with NPC-TW 04 cells. 2135 SVWSATFLSSSP 1 Phage display (common panning) 6 PMID:15670871 PED3 domain of PMCA1b Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2136 FRPNRAQDYNTN(24) 1 Phage display (subtractive panning) 6 PMID:15671538 Prostate Carcinoma Cell Line DU-145 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) The library was added to 293 cells for a negative selection. The medium was collected and centrifuged, and the supernatant was transferred to DU-145 cells. 2137 CPWWSPHAC CPIWQPHYC 2 Phage display (common panning) 6 PMID:15680248 Extracellular domain 1 of plasma membrane Ca2+-pump isoform 4 Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) 2138 YCPRYVRRKLENELLVL(2)[2.217/3.521] PCLMYSKYNLLYPYPAY(1)[2.029] GCMMYRYPAPGNHHIQM(1)[5.101] RCCTRKRFLRWYGHSLL(1)[2.107] SCHFEALRRQTYMYFYV(1)[2.117] 5 Phage display (common panning) 3 PMID:15806288 The ECV304 cells injured by ox-LDL Not determined. Not determined. Not determined. XCX15 phage display library ELISA Fourteen clones randomly selected from the third screening eluates were subjected to ELISA analysis. Absorbance at 450 nm was measured by using an automatic spectrometer, and the absorbance ratio of phage binding to injured ECV304 cells versus control ECV304 cells was calculated and reproduced from the graph. Positive phage clones were designated as those with an absorbance ratio of equal to or higher than 2. 2139 CPSLRLFNC(2) CTTLSTQIC(1) CEQTQTLQC(1) CTLTPKNIC(1) CTAPTVNLC(1) CSDLYLNFC(1) CMDTAYPHC(1) CQLVSYKEC(1) CFAVDEQEC(1) CFKQVRHAC(1) CLATRPPDC(1) CSDSLTPQC(1) 12 Phage display (in vivo) 1 PMID:16259560 Medullary thyroid carcinoma, MTC Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) A Ph.D.-C7C phage display peptide library was injected via the tail vein into tumor-bearing RET-transgenic mice. 2140 CLGEPRAHC(1) CSRESPHPC(13) 2 Phage display (in vivo) 2 PMID:16259560 Medullary thyroid carcinoma, MTC Not determined. Not determined. Not determined. Ph.D.-C7C phage display library (CX7C) A Ph.D.-C7C phage display peptide library was injected via the tail vein into tumor-bearing RET-transgenic mice. 2141 TAWSEVLHLLSR 1 Phage display (common panning) 7 PMID:16452157 PED1 of PMCA4 Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) 2142 SAKTAVSQRVWLPSHRGGEP(22) KSREHVNNSACPSKRITAAL(1) WLSEAGPVVTVRALRGTGSW(2) 3 Phage display (common panning) 3 PMID:16569591 B lymphocyte A20 cell line Not determined. Not determined. Not determined. ON543 phage display library (X20) 2143 SAKTAVSQRVWLPSHRGGEP(8) KSREHVNNSACPSKRITAAL(1) WLSEAGPVVTVRALRGTGSW(4) 3 Phage display (common panning) 4 PMID:16569591 B lymphocyte A20 cell line Not determined. Not determined. Not determined. ON543 phage display library (X20) 2144 CLSDGKRKC CLDGGRPKC 2 Phage display (subtractive panning, in vivo) 2-3 PMID:16740707 C8161 xenograft tumors Not determined. Not determined. Not determined. CX7C T7 phage display library Normal prostate cells were incubated with plaque-forming units (pfu) of T7 phage displaying a CX7C peptide library.After being centrifuged, the supernatant (the normal prostate-subtracted phage library) was recovered and then incubated with cells derived from premalignant prostate tissue or prostate tumor. After subtractive panning, the library was subjected to two to three rounds of in vivo selections. 2145 GSPQCPGGFNCPRCDCGAGY(56) GTGSCGYGKLHTGYWCSYFP(4) VHMNCSWMRVSEGHPCESAD(3) NSSSCDTSVVRSTWACILQP(3) KYGLCRDETVFPSHSCTFTG(3) VYAQCGVNVRTGRGGCSRLM(2) NRLKCRAQATHSAAPCIRGY(2) RQNSCTYSDARRWALCWSGE(2) QLNSCIHSGDRAIRGCMDWV(1) VRAVCTTLKSRGHEECWSLQ(1) GRQGCYEHLWRLIAWCAIFL(1) LRMTCAFGVAQRSADCALSS(1) SIVNCSAALTDLPTRCGGNI(1) CGTRCVRCQNGPEASCEQPL(1) TPLFCGNHGRQPSPLCMKWD(1) FTTVCRQPRGHEAIVCGSGK(1) APSFCGTAMLGASRYCYSGP(1) GARECESGGPGMRKLCTQIN(1) NNRACFRTSKGNPAECPYLG(1) GSLACQNIVVCVKKQCNALC(1) KRASCQNPLFSNFFVCGLSE(1) LPNFCMDTSGRAGPLCMGSE(1) RHTVCRVSLSSVQGSCSHEY(1) H-S-[AG]-x(3)-I-R-G, SAA, S-C-T-[YF]-[ST], R-G-H-E-[EA], [TS]-G-R-[GA]-G-x(3)-[LM], R-V-S-x(5)-[LM], P-x-F-C-x(8)-P-L-C-M-x(2)-[DE], TVCR, DTS 23 Phage display (in vivo) 2 PMID:16885375 Human breast cancer tumor Not determined. Not determined. Not determined. X4CX10CX10 FUSE5 phage display The phage aliquot was added to physiologic saline, i.v. administered and then infused into the patient. 2146 RRKRMTILKSRM TRTKLPRLHLQS 2 Phage display (in vivo) 4 PMID:16967280 B16-F10 tumors Not determined. Not determined. Not determined. Ph.D.-12 phage display library (X12) C57BL/6 mice bearing single, subcutaneous B16-F10 tumors were injected i.p. with the Ph.D.-12TM filamentous bacteriophage phage display peptide library. 2147 GTRQGHTMRLGVSDG[15.00, 0.89] GDVWLFKTSTSHFAR[12.50, 0.14] LWVFPAGGHLGRERS[6.92, NT] VMVPYSVRDSLFGSF[2.08, NT] SVGGWFRQHLVGTRM[5.00, 0.63] LASIVRWEQVPDALS[1.75, 0.06] SGVWAPTAGYDAGFH[2.00, 0.65] IAGLATPGWSHWLAL[1.67, 2.14] SHSDYRPAPWSGWML[4.00, 0.89] 9 Phage display (subtractive panning, in vivo) 4 PMID:16984734 PC-3 human prostate cancer cell xenografts Not determined. Not determined. Not determined. f3-15mer phage display library (X15) Micropanning assay Phage collected from in vivo phage display selections were screened for specificity of binding to both PC-3 –derived tumor tissues and cultured PC-3 human carcinoma cells. In the first round of micropanning, phage were incubated with either excised normal muscle tissues or PC-3 tumor tissues. Bound phage were then eluted from tissues. The amount of eluted phage was then determined by phage titer, and the tumor-tissue-to-normal-tissue ratio was then calculated. All phage clones with a ratio of >1.5 were then used in a second round of micropanning with cultured cell lines. Phage were incubated with cultured HEK293 cells (nonrelevant control) or cultured PC-3 human carcinoma cells. Bound phage were again eluted and titered, and the cultured PC-3 cells/HEK293 cells ratio was calculated. NT denotes not tested. The 15-amino-acid fUSE5 library was injected into the tail vein of normal non-tumor-bearing CF-1 mice to preclear phage that bound to the normal vasculature and other nontumor antigens. The mice were then anesthetized, and blood was obtained and phages were isolated from the blood. Next, the precleared phage library was injected into SCID mice bearing PC-3 human prostate cancer cell xenografts. 2148 KTLLPTP SGVEFLH SKKDTHH TMAPSIK TQHQVTA VNDRNVK 6 Phage display (subtractive panning) 4 PMID:18416599 Mouse PDAC cells Not determined. Not determined. Not determined. Ph.D.-7 phage display library (X7) ELISA Thirty individual phage plaques were isolated and an ELISA was performed to identify the most selective phage for PDAC cells. Of the 30 phage clones analyzed, 16 phage clones (53%) had specificity for PDAC cells. We sequenced the top seven clones on the basis of ELISA and multidimensional analysis. Clones 27 and 5 share identical peptide sequences (KTLLPTP) and demonstrated ideal affinity and specificity for the target PDAC cells. Besides, the extent of phage clone binding and specificity for mouse PDAC and normal ductal cells was quantified via flow cytometry. Clone 27 sharing peptide sequences KTLLPTP was highly specific for mouse PDAC cells having a 112-fold specificity over normal ductal cells. Phage clone 15 sharing peptide sequences TMAPSIK was second in affinity with the rest having nearly identical specificity. The counterselection was done by incubating the internalized phage pool with normal pancreatic cells to subtract all clones that bind to both normal pancreatic ductal cells and PDAC. Then the internalized phage were ampli?ed in Escherichia coli, titered, and subjected to three additional rounds for a total of four rounds of positive selection on the PDAC cells. 2149 NSLTPCGRTRVTSC(2) NSLTPCRNPKKATC(2) NSLTPCGRTRDN(2) NSLTPCAREVTLLC(1) NSLTPCSREVTLLC(1) NSLTPCDTTIANCC(1) NSLTPCDRILSPSC(1) NSLTPCTPKKSGRC(1) NSLTPCTTSSLTDC(1) NSLTPCSTKRKPNC(1) NSLTPCGSNSLTPC(1) NSLTPCCKSLRPHC(1) NSLTPCTKPKRNNC(1) NSLTPCSTKRKPNC(1) 14 Phage display (common panning) 4 PMID:18564683 Anti-LPS monoclonal antibody 9D5 Lipopolysaccharide, LPS Not determined. Not determined. NSLTPCX7C T7 phage display library 2150 NSLTPCGRTRVTSC(4) NSLTPCAPRSSNRC(3) NSLTPCAREVTLLC(2) NSLTPCLLLAQTDC(1) NSLTPCNSKKIPTC(1) NSLTPCLGRISPPC(1) NSLTPCASNSLTPC(1) NSLTPCGRTRDN(1) NSLTPCAPQ(1) 9 Phage display (common panning) 4 PMID:18564683 Anti-EPS monoclonal antibody 4B11 Exopolysaccharide, EPS Not determined. Not determined. NSLTPCX7C T7 phage display library