2076 IgE from patients with allergy to Scylla paramamosain FLN c Protein>Polyclonal antibody Homo sapiens (Human):9606 2077 Spinal Cord Organ and tissue Mus musculus (Mouse):10090 2078 Bone mesenchymal stem cell (BMSC) Cell Rattus norvegicus (Rat):10116 2079 Anti-aflatoxin B1 (AFB1) monoclonal antibody (MAb) 2F5 Protein>Monoclonal antibody Mus musculus (Mouse):10090 BALB/c mice were also immunized by AFB1-BSA conjugation and anti-AFB1 monoclonal antibody (MAb) 2F5 was produced by hybridoma techniques. 2080 Apical membrane antigen-1 Protein>Others F8V414 Eimeria tenella (Coccidian parasite):5802 The extracellular domain gene of Eimeria tenella apical membrane antigen-1 (EctoAMA1) was purified and used as the target. 2081 Tubulin alpha chain Protein>Others A4ZYP4 Phytophthora capsici:4784 The recombinant alpha-tubulin protein from P. capsici was purified individually to homogeneity using Ni-NTA sepharose affinity chromatography. Analysis of the purified recombinant protein was confirmed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) under reducing condition. 2082 Tubulin beta chain Protein>Others A4ZYP2 Phytophthora capsici:4784 The recombinant beta-tubulin protein from P. capsici was purified individually to homogeneity using Ni-NTA sepharose affinity chromatography. Analysis of the purified recombinant protein was confirmed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) under reducing condition. 2083 Lipid A Organic molecules or materials Unknown Gram-negative bacterial lipopolysaccharides are comprised of an outermost highly variable "O-antigenic" oligosaccharide region, relatively less variable middle core oligosaccharide region, and the most invariant innermost hydrophobicpor tion "lipid A". Lipid A accounts for all the toxic effects of endotoxin. 2084 B-domain deleted form of human factor VIII (BDDrFVIII) Protein>Others P00451 Alternative name(s) for factor VIII: Antihemophilic factor AHF Procoagulant component Homo sapiens (Human):9606 1CFG, 1D7P, 1FAC, 1IQD, 2R7E, 3CDZ, 3HNB, 3HNY, 3HOB, 3J2Q, 3J2S, 4BDV, 4KI5, 4PT6, 4XZU, 5K8D Recombinant B-domain deleted factor VIII was obtained from Wyeth BioPharma (Andover, MA, USA). For use in phage selection and screens, this material was lightly biotinylated, using NHS-LC-biotin (Pierce No.21335), with no loss of activity. 2085 Aflatoxin nanobody Nb28 Protein>Others None The AFB1-specific nanobody Nb28 was selected from a phage-displayed library of nanobody (Nb). 2086 Human prostate cancer cell line LNCaP Cell Homo sapiens (Human):9606 The human prostate cancer cell line LNCaP (RCB2144) was provided by the RIKEN BioResource Research Center (BRC). 2087 Gallium ion Inorganic molecules or materials None Gallium ions were applied to the system in form of Ga(NO3)3 diluted to a concentration of 10 mM in the equilibration buffer. Gallium was immobilized at a slower flow rate of 0.34 ml/min for 10 ml through the ion exchanger column. 2088 Human colorectal cancer cell line RKO Cell Homo sapiens (Human):9606 The human epithelial colorectal cancer (CRC) cell line RKO (ATCC CRL 2577) was cultured in Dulbecco’s Modified Eagle Medium (DMEM, Biochrom). 2089 Aromatic-L-amino-acid decarboxylase (EC:4.1.1.28), AADC Protein>Others P20711 Homo sapiens (Human):9606 3RBF, 3RBL, 3RCH 2090 D2-MT1-MMP (MT-loop deleted MMP-14) Protein>Others MMP-14 alternative name(s): Matrix metalloproteinase-14 Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1 Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP MMP-X1 Homo sapiens (Human):9606 2091 Proheparin-binding EGF-like growth factor Protein>Others Q99075 Homo sapiens (Human):9606 1XDT, 2M8S 2139 Invasive U87MG glioblastoma cell line Homo sapiens (Human):9606 U87MG cells were separated into U87T and U87R subpopulations using in vivo selection. Invasive U87R cells were as the target. 2140 SUS316L stainless steel Inorganic molecules or materials None SUS316L disks [;10 9 1.0 mm, Rare Metallic Co., Ltd. (Tokyo, Japan)] was polished with a 400-grit metal file. To remove surface contaminants, the disks were ultrasonically cleaned in acetone for 15 min three times, then irradiated using an UV/ozone cleaner for 30 min. 2141 Escherichia coli O157:H7 cells Escherichia coli O157:H7:83334 Escherichia coli O157:H7 (E. coli O157:H7) is the predominant virulent serotype in a pathogenic subset of Enterohemorrhagic E. coli (EHEC), and is especially founded responsible for serious foodborne infection outbreaks. The control of E. coli O157:H7 infection outbreaks requires rapid and sensitive detections of microbial contamination. 2142 Non-structural protein 1, NS1 Protein>Others P29990 Unknown 2R29,3EVG,6FLA DENV NS1 is a multifunctional non-structural protein indispensable for virus production, as a new target for inhibition of DENV infection by specific peptides.NS1 is a key viral protein that is required for both DENV replication and dengue disease pathogenesis. The NS1 protein comprises of 352 amino acid residues that share approximately 70% sequence similarity among all four DENV serotypes and 40–50% sequence similarity to other flaviviruses. 2138 Brain tumor initiating cells (BTICs) Homo sapiens (Human):9606 Brain tumor initiating cells (BTICs) were supplied by the BTIC Core Facility housed at the University of Calgary. 2137 Anti-fibronectin-binding protein A (FnBPA) N-terminal A domain (FnBPA-A) polyclonal antibody Protein>Polyclonal antibody Oryctolagus cuniculus (Rabbit):9986 New Zealand white rabbits were immunized via multiple subcutaneous injections with 0.5 mg of purified rFnBPA-A protein emulsified with an equal volume of Freund's complete adjuvant (Sigma), followed by boosts with the same dose at 2-week intervals. On the 28th day after primary immunization, the cardiac blood from immunized rabbits was collected, and the immune serum was isolated from coagulated blood. Anti-FnBPA-A antibodies in the immune serum were purified using a HiTrap Protein G HP Column (Pharmacia, Sweden) according to the manufacturer's instructions. 2135 Glass dish Inorganic molecules or materials None 2136 Patients' sera pool containing Alternaria specific IgE (s-IgE) Miscellaneous Homo sapiens (Human):9606 We established a patients' sera pool of Alternaria specific IgE (s-IgE) to screen mimotopes matching B cell epitopes on the surface of Alternaria allergens by phage display technology. Thirty patients monosensitized to Alternaria and suffering from allergic rhinitis and/or asthma (s-IgEs to Alternaria ≥ 3.5 KUA/L, ImmunoCAP 250; Thermo Fisher Scientific/Phadia) were selected. Those with positive s-IgEs to other allergens (s-IgE ≥ 0.35 KUA/L), such as pollen, house dust mites, pets, and foods, were excluded from our study. Blood samples were obtained, and then serum was isolated and mixed to establish an Alternaria sera pool. The s-IgE level was measured. 2134 Trans-2-nonenal Organic molecules or materials Unknown Trans-2-nonenal is a fatty aldehyde with a long-chain, containing nine carbons. It was known that trans-2-nonenal is a cause of the odor associated with aging, and mainly found from the ages of 40 years.